Nine endosulfan degrading bacterial strains were isolated by soil enrichment with endosulfan. Bacterial strain M3 was the most efficient degrader. Endosulfan degradation was accompanied by a decrease in pH of the medium and an increase in chloride ion concentration. The bacterium was tested for its ability to degrade endosulfan at different concentrations in broth and soil. Maximum degradation occurred at concentrations of 50 μg/ml of broth and 100 μg/g of soil. Values of Ks and Vmax were different for (α)- and (β)-endosulfan in broth. The kinetic indices (Vmax/Ks) for α-endosulfan and β-endosulfan were 0.051 and 0.048 day−1 respectively, indicating that (α)-endosulfan was more rapidly degraded. Bacterial strain M3 was identified as Klebsiella sp. M3 on the basis of 16S rDNA sequence similarity (GenBank accession number JX273762).
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Awasthi N, Ahuja R, Kumar A (2000) Factors influencing biodegradation of soil applied endosulfan isomers. Soil Biol Biochem 32:1697–1705
Futamata H, Nagano Y, Watanabe K, Hiraishi A (2005) Unique kinetic properties of phenol-degrading Variovorax strains responsible for efficient trichloroethylene degradation in a chemostat enrichment culture. Appl Environ Microbiol 71:904–911
Goswami S, Singh DK (2009) Biodegradation of α and β endosulfan in broth medium and soil microcosm by bacterial strain Bordetella sp. B9. Biodegradation 20:199–207
Kovarova-Kovar K, Egli T (1998) Growth kinetics of suspended microbial cell: from single substrate-controlled growth to mixed substrate kinetics. Microbial Mol Biol Rev 62:646–666
Kumar M, Philip L (2006) Endosulfan mineralization by bacterial isolates and possible degradation pathway identification. Biorem J 10:179–190
Kumar M, Lakshmi V, Khanna S (2008) Biodegradation and bioremediation of endosulfan contaminated soil. Bioresour Technol 99:3116–3122
Li W, Dai Y, Xue B, Li Y, Peng X, Zhang J, Yan Y (2009) Biodegradation and detoxification of endosulfan in aqueous medium and soil by Achromobacter xylosoxidans strain CS5. J Hazard Mater 167:209–216
Michael TW, Jen-NI K, Craig B, Grant S, Graham VA, John P (2005) Toxicity of alpha-, beta-, (alpha + beta)-endosulfan and their formulated and degradation products to Daphnia magna, Hyalella azteca, Oncorhynchus mykiss, Oncorhynchus kisutch, and biological implications in streams. Environ Toxicol Chem 24:1146–1154
Rao DMR, Murty AS (1980) Persistence of endosulfan in soils. J Agric Food Chem 28:1099–1101
Saiyed H, Dewan A, Bhatnagar V, Shenoy U, Shenoy R, Rajmohan H, Patel K, Kashyap R, Kulkarni P, Rajan B, Lakkad B (2003) Effect of endosulfan on male reproductive development. Environ Health Perspect 111:1958–1962
Shivaramaiah HM, Kennedy IR (2006) Biodegradation of endosulfan by a soil bacterium. J Environ Sci Health Part B 41:895–905
Sutherland TD, Horne J, Lacey MJ, Harcourt R, Russell RJ, Oakeshott JG (2000) Enrichment of an endosulfan-degrading mixed bacterial culture. Appl Environ Microbiol 66:2822–2828
Sutherland TD, Horne I, Russell RJ, Oakeshott JG (2002) Gene cloning and molecular characterization of a two-enzyme system catalyzing the oxidative detoxification of β-endosulfan. Appl Environ Microbiol 68:6237–6245
Verma K, Agrawal N, Farooq M, Misra RB, Hans RK (2006) Endosulfan degradation by a Rhodococcus strain isolated from earthworm gut. Ecotoxicol Environ Saf 64:377–381
Weir KM, Sutherland TD, Horne I, Russell RJ, Oakeshott JG (2006) A single monooxygenase, Ese, is involved in the metabolism of the organ chlorides endosulfan and endo sulphate in an Arthrobacter sp. Appl Environ Microbiol 72:3524–3530
We are highly thankful to the funding agency, Indian Council of Agricultural Research (ICAR) for providing funds under NFBSFARA (NFBSFARA/WQ-2023/2012-13). Ms. Madhu is thankful to CSIR, Delhi for providing the fellowship as JRF/SRF.
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Singh, M., Singh, D.K. Biodegradation of Endosulfan in Broth Medium and in Soil Microcosm by Klebsiella sp. M3. Bull Environ Contam Toxicol 92, 237–242 (2014). https://doi.org/10.1007/s00128-013-1168-3
- Enzyme kinetics
- Klebsiella sp. M3