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Biological Effects of Pyrimethinal on Aquatic Worms (Tubifex tubifex) Under Laboratory Conditions

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Abstract

Laboratory studies were conducted to determine the effects of different concentrations of pyrimethinal on protein contents, and some oxidative stress in Tubifex tubifex after an exposure of 2, 4, and 7 days. Residues of the fungicide were followed in water and in the worms. In water, pyrimethinal concentration decreased slowly (maximum −6.4 % ± 0.8 % after 2 days for 25 mg L−1). In the worms, it increased after 4 days and decreased thereafter. LC50 values were between 49.2 ± 0.58 and 39.5 ± 0.95 mg L−1 depending on exposure time. The activity of catalase increased in response to the fungicide after 2 days of exposure to 25 mg L−1 of pyrimethinal (+90 %). The highest decrease of glutathione-S-transferase activity (−29.7 %) was found after 7 days in the presence of 25 mg L−1.

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References

  • Almar MM, Mayans JD, Rmero FJ (1987) Glutathion content and GSH S-transferase activity in mid gut gland of Procambarus clarkii, sex difference, the effect of fasting and their implications in cadmium toxicity. Comp Biochem Phys 87C:433–440

    CAS  Google Scholar 

  • Anderson ME, Luo JL (1998) Glutathione therapy: from pro drugs to genes. Semin Liver Dis 41:1309–1319

    Google Scholar 

  • Anderson C, Brumhard B, Ditgens K, Reiner H (1999) Metabolism of fenhexamid (KBR 2738) in plants, animals, and the environment. Pflanzenschutz Nachr Bayer 52:227–251

    Google Scholar 

  • Bradford MN (1976) A rapid and sensitive method for the quantitation of micrograms of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Claiborne A (1985) Catalase activity. In: Greenwald RA (ed) Handbook of methods in oxygen radical research. CRC Press, Boca-Raton, pp 283–284

    Google Scholar 

  • Cossu C, Doyotte A, Jacquin MC, Vasseur P (1997) Biomarqueurs du stress oxydant chez les animaux aquatiques. In: Lagadic L, Caquet T, Amiard JC, Ramade F (eds) Biomarqueurs en Ecotoxicologie: Aspects Fondamentaux. Masson, Paris, pp 149–161

    Google Scholar 

  • Davies JM, Lowry CV, Davies KJA (1994) Free radicals. In: Nohl H, Esterbauer H, Rice-Evans C (eds) Environment medicine and toxicology. Richelieu Press, London, pp 563–578

    Google Scholar 

  • Egeler P, Rombke M, Meller M, Knacker T, Franke C, Studinger G, Nagel R (1997) Bioaccumulation of lindane and hexachlorobenzene by tubifcid sludge worms (Oligochaeta) under standardized laboratory conditions. Chemosphere 35:835–852

    Article  CAS  Google Scholar 

  • Gillis BL, Diener LC, Reynoldson TR, Dixon DG (2002) Cadmium-induced production of a metallothionein like protein in Tubifex tubifex (Oligochaeta) and Chironomus riparius (Diptera): correlation with reproduction and growth. Environ Toxicol Chem 21:1836–1844

    Article  CAS  Google Scholar 

  • Gillis PL, Reynoldson TB, Dixon DG (2004) Natural variation in a metallothionein-like protein in Tubifex tubifex in the absence of metal exposure. Ecotoxicol Environ Saf 58:22–28

    Article  CAS  Google Scholar 

  • Hazarika A, Sarkar SN (2001) Effect of isoproturon pretreatment on the biochemicaltoxicodynamics of anilofos in male rats. Toxicology 165:87–95

    Article  CAS  Google Scholar 

  • Lagauzère S, Boyer P, Stora G, Bonzom JM (2009) Effects of uranium-contaminated sediments on the bioturbation activity of Chironomus riparius larvae (Insecta, Diptera) and Tubifex tubifex worms (Annelida, Tubificidae). Chemosphere 76:324–334

    Article  Google Scholar 

  • Lucan-Bouché ML, Biagianti-Risbourg S, Arsac F, Vernet G (1999) An original decontamination process developed by the aquatic oligochaete Tubifex tubifex exposed to copper and lead. Aquat Toxicol 45:9–17

    Article  Google Scholar 

  • Maestre Z, Martinez-Madrid M, Rodriguez P (2009) Monitoring the sensitivity of the oligochaete Tubifex tubifex in laboratory cultures using three toxicants. Ecotoxicol Environ Saf 72:2083–2089

    Article  CAS  Google Scholar 

  • Mosleh YY, Ismail SS, Ahmed MT, Ahems YM (2003a) Comparative toxicity and biochemical responses of certain pesticides on mature eartworms Aporrectodea caliginosa under laboratory conditions. Environ Toxicol 18:338–346

    Article  CAS  Google Scholar 

  • Mosleh YY, Paris-Palacios S, Couderchet M, Vernet G (2003b) Effects of the herbicide isoporoturon on survival, growth rate, and protein content of mature earthworms (Lumbricus terrestris L.) and its fate in the soil. Appl Soil Ecol 23:69–77

    Article  Google Scholar 

  • Mosleh YY, Paris-Palacios S, Arnoult A, Couderchet M, Biagianti-Risbourg S, Vernet G (2004) Metallothionein induction in the aquatic Oligocheate Tubifex tubifex exposed to the herbicide isoproturon. Environ Toxicol 19:88–93

    Article  CAS  Google Scholar 

  • Mosleh YY, Paris-Palacios S, Couderchet M, Biagianti-Risbourg S, Vernet G (2005) Effects of the herbicide isoproturon on metallothioneins, growth and antioxidative defences in the aquatic worm Tubifex tubifex (Oligochaeta, Tubificidae). Ecotoxicology 14:1–13

    Article  Google Scholar 

  • Mosleh YY, Paris-Palacios S, Biagianti-Risbourg S (2006) Metallothioneins induction and antioxidative response in aquatic worms Tubifex tubifex (Oligochaeta, Tubificidae) exposed to copper. Chemosphere 64:121–128

    Article  CAS  Google Scholar 

  • Mosleh YY, Paris-Palacios S, Mohamed TA, Mahmoud F, Osman MA, Biagianti-Risbourg S (2007) Effects of chitosan on oxidative stress and metallothioneins in aquatic worm Tubifex tubifex (Oligochaeta, Tubifcidae). Chemosphere 67:167–175

    Article  CAS  Google Scholar 

  • Organisation for Economic Co-operation and Development (OECD) (1984) Earthworm, acute toxicity test. Guideline No. 207. Organisation for Economic Co-operation and Development, Paris

  • Paris-Palacios S, Mosleh Y, Mohamad A, Laurence DT, Conrad A, Arnoult F, Biagianti-Risbourg S (2010) Toxic effects and bioaccumulation of the herbicide isoproturon in Tubifex tubifex (Oligocheate, Tubificidae): a study of significance of autotomy and its utility as a biomarker. Aquat Toxicol 98:8–14

    Article  CAS  Google Scholar 

  • Regoli F, Nigro M, Bertoli E, Principato J, Orlando E (1997) Defences against oxidative stress in the Antartic scallop Adamussium colbecki and effects of acute exposure to metals. Hydrobiologia 355:139–144

    Article  CAS  Google Scholar 

  • Ribeiro S, Sousa JP, Noguerira AJ, Soares AM (2001) Effect of endosulfan and parathion on energy reserves and physiological parameters of the terrestrial isopod Porcellio dilatatus. Ecotoxicol Environ Saf 49:131–138

    Article  CAS  Google Scholar 

  • Saravana-Bhavan P, Geraldine P (2000) Aberration in various parameters of bioenergetic in the Macrobarchium malcomsonii following exposure to endosulfan. Aquaculture 1:141–150

    Google Scholar 

  • Saravana-Bhavan P, Geraldine P (2001) Biochemical stress responses in tissues of the prawn Macrobarchium malcomsonii on exposure to endosulfan. Pestic Biochem Phys 70:27–41

    Article  Google Scholar 

  • Widianarko B, Van Straalen N (1996) Toxicokinetics-based survival analysis in bioassays using non-persistent chemicals. Environ Toxicol Chem 15:402–406

    Article  CAS  Google Scholar 

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Correspondence to Yahia Youssef Mosleh.

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Mosleh, Y.Y., Mofeed, J., Afifi, M. et al. Biological Effects of Pyrimethinal on Aquatic Worms (Tubifex tubifex) Under Laboratory Conditions. Bull Environ Contam Toxicol 92, 85–89 (2014). https://doi.org/10.1007/s00128-013-1153-x

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  • DOI: https://doi.org/10.1007/s00128-013-1153-x

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