Histological Alterations on the Structure of the Excretory Renal System in Tench (Tinca tinca) After Exposure to 17-Alpha-Ethynylestradiol

Abstract

This study describes the effects of 17-alpha-ethynylestradiol (EE2) on the structure of the excretory system of the kidney in tench. Adult male tench were exposed to sub-lethal doses of EE2 (50, 100 and 500 μg/kg b.w.) under semistatic conditions for a period of 30 days. The nephrosomatic index and histology (including a morphometric analysis) of the kidney were examined. Histopathological lesions in the kidney of exposed tench were: dilation of glomerular capillaries and increase in the area of the renal corpuscle, hyaline degeneration in the epithelial cells of the proximal tubules leading to necrotic changes, hemorrhages in the interstitial tissue and deposits of eosinophilic material. These lesions were observed with a greater degree of severity as the exposure doses were increased. These results indicate that long-term exposure to EE2 could produce clear negative effects on the excretory system of the kidney in tench and consequently on their physiological functions.

This is a preview of subscription content, access via your institution.

Fig. 1

References

  1. Caille N, Rodina M, Kocour M, Gela D, Flajšhans M, Linhart O (2006) Quantity, motility and fertility of tench Tinca tinca (L.) sperm in relation to LHRH analogue and carp pituitary treatments. Aquacult Int 14:75–87

    CAS  Article  Google Scholar 

  2. Ferguson HW (1989) Systemic pathology of fish. A text and atlas of comparative tissue responses in diseases of teleosts, 1st edn. Iowa State University Press, Ames

    Google Scholar 

  3. Herman RL, Kincaid HL (1998) Pathological effects of orally administered estradiol to rainbow trout. Aquaculture 72:165–172

    Article  Google Scholar 

  4. Hibiya T (1982) Atlas of fish histology. Normal and pathological features. Editorial Kodhansa Scientific Books, Tokyo

    Google Scholar 

  5. Länge R, Hutchinson TH, Croudace CP, Siegmund F, Schweinfurth H, Hampe P, Panter GH, Sumpter JP (2001) Effects of the synthetic estrogen 17α-ethynylestradiol on the life-cycle of the fathead minnow (Pimephales promelas). Environ Toxicol Chem 20:1216–1227

    Article  Google Scholar 

  6. Larsson DGJ, Adolfsson-Erici M, Parkkonen J, Pettersson M, Berg AH, Olsson PE, Förlin L (1999) Ethinyloestradiol—an undesired fish contraceptive. Aquat Toxicol 45:91–97

    CAS  Article  Google Scholar 

  7. López de Alda MJ, Gil A, Paz E, Barceló D (2002) Ocurrence and analysis of estrogens and progestogens in river sediments by liquid chromatography-electrospray-mass spectrometry. Anal 127:1299–1304

    Article  Google Scholar 

  8. Magri MacMahon AF (1946) Fishlore. Pelican Books, Gretna

    Google Scholar 

  9. Palace VP, Wautier KG, Evans RE, Blanchfield P, Mills KH, Chalanchuk SM, Godard D, McMaster ME, Tetreault GR, Peters LE, Vandenbyllaardt L, Kidd KA (2006) Biochemical and histopathological effects in pearl dace (Margariscus margarita) chronically exposed to a synthetic estrogen in a whole lake experiment. Environ Toxicol Chem 25(4):1114–1125

    CAS  Article  Google Scholar 

  10. REAL DECRETO 1201/2005, de 10 de octubre, sobre protección de los animales utilizados para experimentación y otros fines científicos. B.O.E. no 252. Ministerio de la Presidencia. Madrid (Spain)

  11. Schwaiger J, Spieser OH, Bauer C, Ferling H, Mallow U, Kalbfus W, Negele RD (2000) Chronic toxicity of nonylphenol and ethynylestradiol: haematological and histopathological effects in juvenile common carp (Cyprinus carpio). Aquat Toxicol 51:69–78

    CAS  Article  Google Scholar 

  12. Tong J, Yu X, Liao X, Wang J, Hu S, Guan M, Linhart O, Flajshans M (2002) Preliminary survey of genetic variation of tench (Tinca tinca) from the Czech Republic and China. In: Proceedings XXth genetic days 303–304

  13. Weber LP, Hill RL, Janz DM (2003) Developmental estrogenic exposure in zebrafish (Danio rerio): II. Histological evaluation of gametogenesis and organ toxicity. Aquat Toxicol 63:431–446

    CAS  Article  Google Scholar 

  14. Xu J, Wu LS, Chen WP, Jiang PP, Chang ACS (2009) Pharmaceuticals and personal care products (PPCPs), and endocrine disrupting compounds (EDCs) in runoff from a potato field irrigated with treated wastewater in southern California. J Health Sci 55:306–310

    CAS  Article  Google Scholar 

  15. Zar JH (1996) Biostatistical analysis. Prentice Hall International Editions, New Jersey

    Google Scholar 

  16. Zaroogian G, Gardner G, Horowitz BD, Gutjahr-Gobell R, Haebler R, Mills L (2001) Effect of 17β-estradiol, o, p-DDT, octylphenol and p, p-DDE on gonadal development and liver and kidney pathology in juvenile male summer flounder (Paralochthys dentatus). Aquat Toxicol 54:101–112

    CAS  Article  Google Scholar 

  17. Zha J, Wang Z, Wang N, Ingersoll C (2007) Histological alternation and vitellogenin induction in adult rare minnow (Gobiocypris rarus) after exposure to ethynylestradiol and nonylphenol. Chemosphere 66:488–495

    CAS  Article  Google Scholar 

  18. Zhang X, Li QZ, Li GX, Wang ZS, Yan CZ (2009) Levels of estrogenic compounds in Xiamen Bay sediment, China. Mar Pollut Bull 58:1210–1216

    CAS  Article  Google Scholar 

Download references

Acknowledgments

This study was supported by the University of Extremadura (Plan de iniciación a la investigación, desarrollo tecnológico e innovación. 2008), Spain.

Author information

Affiliations

Authors

Corresponding author

Correspondence to A. L. Oropesa.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Oropesa, A.L., Jiménez, B., Fallola, C. et al. Histological Alterations on the Structure of the Excretory Renal System in Tench (Tinca tinca) After Exposure to 17-Alpha-Ethynylestradiol. Bull Environ Contam Toxicol 91, 623–629 (2013). https://doi.org/10.1007/s00128-013-1088-2

Download citation

Keywords

  • 17-Alpha-ethynylestradiol
  • Histophatology
  • Kidney
  • Tinca tinca