Histological Alterations on the Structure of the Excretory Renal System in Tench (Tinca tinca) After Exposure to 17-Alpha-Ethynylestradiol

  • A. L. OropesaEmail author
  • B. Jiménez
  • C. Fallola
  • H. J. Pula
  • J. M. Cuesta
  • L. Gómez


This study describes the effects of 17-alpha-ethynylestradiol (EE2) on the structure of the excretory system of the kidney in tench. Adult male tench were exposed to sub-lethal doses of EE2 (50, 100 and 500 μg/kg b.w.) under semistatic conditions for a period of 30 days. The nephrosomatic index and histology (including a morphometric analysis) of the kidney were examined. Histopathological lesions in the kidney of exposed tench were: dilation of glomerular capillaries and increase in the area of the renal corpuscle, hyaline degeneration in the epithelial cells of the proximal tubules leading to necrotic changes, hemorrhages in the interstitial tissue and deposits of eosinophilic material. These lesions were observed with a greater degree of severity as the exposure doses were increased. These results indicate that long-term exposure to EE2 could produce clear negative effects on the excretory system of the kidney in tench and consequently on their physiological functions.


17-Alpha-ethynylestradiol Histophatology Kidney Tinca tinca 



This study was supported by the University of Extremadura (Plan de iniciación a la investigación, desarrollo tecnológico e innovación. 2008), Spain.


  1. Caille N, Rodina M, Kocour M, Gela D, Flajšhans M, Linhart O (2006) Quantity, motility and fertility of tench Tinca tinca (L.) sperm in relation to LHRH analogue and carp pituitary treatments. Aquacult Int 14:75–87CrossRefGoogle Scholar
  2. Ferguson HW (1989) Systemic pathology of fish. A text and atlas of comparative tissue responses in diseases of teleosts, 1st edn. Iowa State University Press, AmesGoogle Scholar
  3. Herman RL, Kincaid HL (1998) Pathological effects of orally administered estradiol to rainbow trout. Aquaculture 72:165–172CrossRefGoogle Scholar
  4. Hibiya T (1982) Atlas of fish histology. Normal and pathological features. Editorial Kodhansa Scientific Books, TokyoGoogle Scholar
  5. Länge R, Hutchinson TH, Croudace CP, Siegmund F, Schweinfurth H, Hampe P, Panter GH, Sumpter JP (2001) Effects of the synthetic estrogen 17α-ethynylestradiol on the life-cycle of the fathead minnow (Pimephales promelas). Environ Toxicol Chem 20:1216–1227CrossRefGoogle Scholar
  6. Larsson DGJ, Adolfsson-Erici M, Parkkonen J, Pettersson M, Berg AH, Olsson PE, Förlin L (1999) Ethinyloestradiol—an undesired fish contraceptive. Aquat Toxicol 45:91–97CrossRefGoogle Scholar
  7. López de Alda MJ, Gil A, Paz E, Barceló D (2002) Ocurrence and analysis of estrogens and progestogens in river sediments by liquid chromatography-electrospray-mass spectrometry. Anal 127:1299–1304CrossRefGoogle Scholar
  8. Magri MacMahon AF (1946) Fishlore. Pelican Books, GretnaGoogle Scholar
  9. Palace VP, Wautier KG, Evans RE, Blanchfield P, Mills KH, Chalanchuk SM, Godard D, McMaster ME, Tetreault GR, Peters LE, Vandenbyllaardt L, Kidd KA (2006) Biochemical and histopathological effects in pearl dace (Margariscus margarita) chronically exposed to a synthetic estrogen in a whole lake experiment. Environ Toxicol Chem 25(4):1114–1125CrossRefGoogle Scholar
  10. REAL DECRETO 1201/2005, de 10 de octubre, sobre protección de los animales utilizados para experimentación y otros fines científicos. B.O.E. no 252. Ministerio de la Presidencia. Madrid (Spain)Google Scholar
  11. Schwaiger J, Spieser OH, Bauer C, Ferling H, Mallow U, Kalbfus W, Negele RD (2000) Chronic toxicity of nonylphenol and ethynylestradiol: haematological and histopathological effects in juvenile common carp (Cyprinus carpio). Aquat Toxicol 51:69–78CrossRefGoogle Scholar
  12. Tong J, Yu X, Liao X, Wang J, Hu S, Guan M, Linhart O, Flajshans M (2002) Preliminary survey of genetic variation of tench (Tinca tinca) from the Czech Republic and China. In: Proceedings XXth genetic days 303–304Google Scholar
  13. Weber LP, Hill RL, Janz DM (2003) Developmental estrogenic exposure in zebrafish (Danio rerio): II. Histological evaluation of gametogenesis and organ toxicity. Aquat Toxicol 63:431–446CrossRefGoogle Scholar
  14. Xu J, Wu LS, Chen WP, Jiang PP, Chang ACS (2009) Pharmaceuticals and personal care products (PPCPs), and endocrine disrupting compounds (EDCs) in runoff from a potato field irrigated with treated wastewater in southern California. J Health Sci 55:306–310CrossRefGoogle Scholar
  15. Zar JH (1996) Biostatistical analysis. Prentice Hall International Editions, New JerseyGoogle Scholar
  16. Zaroogian G, Gardner G, Horowitz BD, Gutjahr-Gobell R, Haebler R, Mills L (2001) Effect of 17β-estradiol, o, p-DDT, octylphenol and p, p-DDE on gonadal development and liver and kidney pathology in juvenile male summer flounder (Paralochthys dentatus). Aquat Toxicol 54:101–112CrossRefGoogle Scholar
  17. Zha J, Wang Z, Wang N, Ingersoll C (2007) Histological alternation and vitellogenin induction in adult rare minnow (Gobiocypris rarus) after exposure to ethynylestradiol and nonylphenol. Chemosphere 66:488–495CrossRefGoogle Scholar
  18. Zhang X, Li QZ, Li GX, Wang ZS, Yan CZ (2009) Levels of estrogenic compounds in Xiamen Bay sediment, China. Mar Pollut Bull 58:1210–1216CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • A. L. Oropesa
    • 1
    Email author
  • B. Jiménez
    • 1
  • C. Fallola
    • 2
  • H. J. Pula
    • 2
  • J. M. Cuesta
    • 3
  • L. Gómez
    • 3
  1. 1.Toxicology Area, School of Veterinary MedicineUniversity of ExtremaduraCáceresSpain
  2. 2.Aquaculture Center, Dirección General de Medio NaturalJunta de ExtremaduraBadajozSpain
  3. 3.Anatomophatology Area, School of Veterinary MedicineUniversity of ExtremaduraCáceresSpain

Personalised recommendations