Assessments were conducted to determine the effect of sample storage method and associated holding time on surface water nutrient concentrations from field sites. Six surface water sites and two nutrient spiked, laboratory water samples were evaluated for nitrate, nitrite, ammonium, filtered orthophosphorus, and total orthophosphorus concentrations on four separate days throughout the period of 1 year. Samples stored at ambient temperature (23°C) for 24 h prior to nutrient analyses resulted in 18 % ± 2 % of results being significantly different from controls (which were analyzed immediately upon collection). Samples placed in the cooler (4°C) for 7 days prior to nutrient analyses resulted in 30 % ± 1 % of values being significantly different from controls. Samples placed in the freezer (−20°C) for 7 days prior to analyses resulted in 34 % ± 12 %, 44 % ± 10 %, and 28 % ± 5.7 % of ammonium, filtered orthophosphate, and total orthophosphate, respectively, values being significantly different from controls. This study highlights the challenges facing researchers in efficient collection, storage and nutrient analysis of samples, especially when sites are remote and difficult to access .
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American Public Health Association (APHA) (2005) Standard methods for the examination of water and wastewater, 21st edn. APHA, Washington, DC
Burke PM, Hill S, Iricanin N, Douglas C, Essex P, Tharin D (2002) Evaluation of preservation methods for nutrient species collected by automatic samplers. Environ Monit Assess 80(2):49–73
Fellman JB, D’Amore DV, Hood E (2008) An evaluation of freezing as a preservation technique for analyzing dissolved organic C, N and P in surface water samples. Sci Total Environ 392:305–312
Fishman MJ, Schroder LJ, Shockey MW (1986) Evaluation of methods for preservation of water samples for nutrient analysis. Intern J Environ Stud 26:231–238
Gardolinski PCFC, Hanrahan G, Achterberg EP, Gledhill M, Tappin AD, House WA, Worsfold PJ (2001) Comparison of sample storage protocols for the determination of nutrients in natural waters. Water Res 35(15):3670–3678
Jarvie HP, Withers PJA, Neal C (2002) Review of robust measurement of phosphorus in river water: sampling, storage, fractionation and sensitivity. Hydrol Earth Syst Sci 6(1):113–132
Kopsky TJ, Volkmar E, Keith L, Dahlgren R (2010) Draft: nutrient holding time study. Regional Water Quality Control Board, Central Valley Region, California Environmental Protection Agency. http://www.swrcb.ca.gov/rwqcb5/water_issues/swamp/water_quality_reports/nhts_draftrpt.pdf
Kotlash AR, Chessman BC (1998) Effects of water sample preservation and storage on nitrogen and phosphorus determinations: implications for the use of automated sampling equipment. Water Res 32(12):3731–3737
Mitchell NL, Lamberti GA (2005) Responses in dissolved nutrients and epilithon abundance to spawning salmon in southeast Alaska streams. Limnol Oceanogr 50(1):217–227
Murphy R, Riley JP (1962) A modified single solution method for the determination of phosphate in natural waters. Anal Chim Acta 27:31–36
Salley BA (1995) A comparison of preservation techniques for dissolved nutrient analyses. Special Report in Applied Marine Science and Ocean Engineering No. 333. Virginia Institute of Marine Sciences, p 44
Triska FJ, Kennedy VC, Avanzino RJ, Zellweger GW, Bencala KE (1989) Retention and transport of nutrients in a third-order stream: channel processes. Ecology 70(6):1877–1892
US Environmental Protection Agency (US EPA) (1983) Sample preservation. In: Methods for chemical analysis of water and wastes. EPA-600/4-79-020. Cincinnati, OH
Vesley J (1990) Stability of the pH and the contents of ammonium and nitrate in precipitation samples. Atmos Environ 24A:3085–3089
Thanks to Lisa Brooks for nutrient analyses. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture (USDA). The USDA is an equal opportunity employer and provider.
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Moore, M.T., Locke, M.A. Effect of Storage Method and Associated Holding Time on Nitrogen and Phosphorus Concentrations in Surface Water Samples. Bull Environ Contam Toxicol 91, 493–498 (2013). https://doi.org/10.1007/s00128-013-1084-6
- Water quality
- Ambient temperature