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Study on the In Vitro Effects of the Mixtures of Polycyclic Aromatic Hydrocarbons (PAHs) and Heavy Metals on Ethoxyresorufin-O-Deethylase (EROD) Activity in Mossambica tilapia Liver


This paper reports in vitro effects of individual heavy metals (Cd2+, Cu2+ and Hg2+), and PAHs, including benzo[a]pyrene(BaP), indeno[1,2,3-cd]pyrene (IP) and fluoranthene (FL), and their mixtures on ethoxyresorufin-O-deethylase (EROD) activities using a plate-reader method. The results showed that all three metals inhibited EROD activity, while BaP/IP significantly induced the enzyme. However, FL alone decreased EROD activity. Moreover, co-treatment with BaP/IP and heavy metals inhibited PAH-induced EROD activities, while combined exposure to FL and heavy metals induced FL-inhibited EROD activities.

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The work was supported by the National Public Benefit (Ocean) Research Foundation of China (201105013), the Grand Research Foundation of Fujian Province(2011YZ0001-1 and 2013J01386), and the Scientific Research Foundation of Third Institute of Oceanography, SOA(201221).

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Correspondence to Hai Yan Wang.

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Han, D.X., Wang, H.Y., Yue, H.W. et al. Study on the In Vitro Effects of the Mixtures of Polycyclic Aromatic Hydrocarbons (PAHs) and Heavy Metals on Ethoxyresorufin-O-Deethylase (EROD) Activity in Mossambica tilapia Liver. Bull Environ Contam Toxicol 91, 460–464 (2013).

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  • Mixture
  • PAHs
  • Heavy metals
  • EROD activity
  • Plate-reader method