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Assessment of In Vivo Estrogenic Response and Identification of Environmental Estrogens in Influent and Effluent from a Sewage Treatment Plant

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Abstract

The in vivo estrogenic response and estrogenic contents of the influent and effluent collected from a sewage treatment plant located in Jiaozuo were assessed. The bioassay showed significant serum vitellogenin (VTG) induction in all the treated male goldfish (Carassius auratus) and significant gonad atrophies were only observed in the fish induced the most VTG expressions. Six target estrogens (estrone, 17β-estradiol, 17α-ethynylestradiol, 4-n-octylphenol, 4-n-nonylphenol and bisphenol A) were detected in different polar fractions, with the exception of the 25 % and 50 % methanol fractions extracted from the influent and the 25 %, 50 %, 95 % and 100 % methanol fractions extracted from the effluent. For both the influent and effluent, natural and synthetic steroidal estrogens were detected in those extracted fractions induced the most abundant VTG expressions.

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Acknowledgments

This research was financially supported by Scientific Research Fund of Henan Provincial Education Department (No. 13A610314, No. 13A440322) and the Doctor Fund of Henan Polytechnic University (No. B2011-056, No. B2010-69).

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Authors and Affiliations

  1. School of Physics and Chemistry, Henan Polytechnic University, Jiaozuo, 454003, People’s Republic of China

    Wenting Song

  2. School of Safety Science and Engineering, Henan Polytechnic University, Jiaozuo, 454003, People’s Republic of China

    Zhijun Wang

  3. Department of Resource and Civil Engineering, Shandong University of Science and Technology, Taian, 271019, People’s Republic of China

    Chuanjie Lian

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  1. Wenting Song
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  2. Zhijun Wang
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  3. Chuanjie Lian
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Correspondence to Wenting Song.

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Song, W., Wang, Z. & Lian, C. Assessment of In Vivo Estrogenic Response and Identification of Environmental Estrogens in Influent and Effluent from a Sewage Treatment Plant. Bull Environ Contam Toxicol 91, 324–329 (2013). https://doi.org/10.1007/s00128-013-1061-0

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  • DOI: https://doi.org/10.1007/s00128-013-1061-0

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