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Identification of ABC Transporter Genes in Gonad Tissue of Two Mediterranean Sea Urchin Species: Black, Arbacia lixula L., and Rocky, Paracentrotus lividus L.

Bulletin of Environmental Contamination and Toxicology volume 91, pages 415–419 (2013)Cite this article

Abstract

Multixenobiotic resistance (MXR) represents an important cellular detoxification mechanism in aquatic organisms as it provides them robustness toward natural and man-made contaminants. Several ABC transporters have major roles in the MXR phenotype – P-gp/ABCB1, MRP1–3/ABCC1–3 and BCRP/ABCG2. In this study, we identified the presence of ABC transporters involved in the MXR mechanism of Arbacia lixula and Paracentrotus lividus. AlABCB1/P-gp, AlABCC3/MRP3, AlABCC9/SUR-like and AlABCG-like transcripts were identified in A. lixula; and PlABCC1/P-gp, PlABCC3/MRP3, PlABCC5/MRP5, and PlABCC9/SUR-like transcripts in P. lividus. For each of the new partial sequences, we performed detailed phylogenetic and identity analysis as a first step toward full characterization and understanding of the ecotoxicological role of these ABC transporters.

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Acknowledgments

This work has been supported by the Ministry of Science, Education and Sports of the Republic of Croatia, Project Nos. 058-0582261-2246 and 098-0982934-2745.

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Authors and Affiliations

  1. Laboratory for Biology and Microbial Genetics, Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000, Zagreb, Croatia

    Ivana Bošnjak, Lidija Šver & Jasna Franekić

  2. Laboratory for Molecular Ecotoxicology, Division for Marine and Environmental Research, Ruđer Bošković Institute, Bijenička cesta 54, 10000, Zagreb, Croatia

    Roko Zaja & Tvrtko Smital

  3. Laboratory for Ichtyopathology - biological materials, Division of Materials Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000, Zagreb, Croatia

    Roberta Sauerborn Klobučar

Authors
  1. Ivana Bošnjak
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  2. Roko Zaja
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  3. Roberta Sauerborn Klobučar
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  4. Lidija Šver
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  5. Jasna Franekić
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  6. Tvrtko Smital
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Corresponding author

Correspondence to Ivana Bošnjak.

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Bošnjak, I., Zaja, R., Klobučar, R.S. et al. Identification of ABC Transporter Genes in Gonad Tissue of Two Mediterranean Sea Urchin Species: Black, Arbacia lixula L., and Rocky, Paracentrotus lividus L.. Bull Environ Contam Toxicol 91, 415–419 (2013). https://doi.org/10.1007/s00128-013-1021-8

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  • DOI: https://doi.org/10.1007/s00128-013-1021-8

Keywords

  • ABC transporters
  • MXR mechanism
  • Arbacia lixula
  • Paracentrotus lividus