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Identification of ABC Transporter Genes in Gonad Tissue of Two Mediterranean Sea Urchin Species: Black, Arbacia lixula L., and Rocky, Paracentrotus lividus L.


Multixenobiotic resistance (MXR) represents an important cellular detoxification mechanism in aquatic organisms as it provides them robustness toward natural and man-made contaminants. Several ABC transporters have major roles in the MXR phenotype – P-gp/ABCB1, MRP1–3/ABCC1–3 and BCRP/ABCG2. In this study, we identified the presence of ABC transporters involved in the MXR mechanism of Arbacia lixula and Paracentrotus lividus. AlABCB1/P-gp, AlABCC3/MRP3, AlABCC9/SUR-like and AlABCG-like transcripts were identified in A. lixula; and PlABCC1/P-gp, PlABCC3/MRP3, PlABCC5/MRP5, and PlABCC9/SUR-like transcripts in P. lividus. For each of the new partial sequences, we performed detailed phylogenetic and identity analysis as a first step toward full characterization and understanding of the ecotoxicological role of these ABC transporters.

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  • Arslan O, Parlak H (2007) Embryotoxic effects of nonylphenol and octylphenol in sea urchin Arbacia lixula. Ecotoxicology 16:439–444

    CAS  Article  Google Scholar 

  • Bard SM (2000) Multixenobiotic resistance as a cellular defense mechanism in aquatic organisms. Aquat Toxicol 48:357–389

    CAS  Article  Google Scholar 

  • Bošnjak I, Uhlinger KR, Heim W, Smital T, Franekić Čolić J, Coale K, Epel D, Hamdoun A (2009) Multidrug efflux transporters limit accumulation of inorganic, but not organic, mercury in sea urchin embryos. Environ Sci Technol 43:8374–8380

    Article  Google Scholar 

  • Bošnjak I, Šegvić T, Smital T, Franekić J, Mladineo I (2011) Sea urchin embryotoxicity test for environmental contaminants – potential role of the MRP proteins. Water Air Soil Pollut 217:627–636

    Article  Google Scholar 

  • Cesar A, Marín A, Marín-Guirao L, Vita R (2004) Amphipod and sea urchin tests to assess the toxicity of Mediterranean sediments: the case of Portmán Bay. Sci Mar 68:205–213

    CAS  Article  Google Scholar 

  • Cole SP, Deeley RG (2006) Transport of glutathione and glutathione conjugates by MRP1. Trends Pharmacol Sci 27:438–446

    CAS  Article  Google Scholar 

  • Epel D, Luckenbach T, Stevenson CN, MacManus-Spencer LA, Hamdoun A, Smital T (2008) Efflux transporters: newly appreciated roles in protection against pollutants. Environ Sci Technol 42:3914–3920

    CAS  Article  Google Scholar 

  • Goldstone JV, Hamdoun A, Cole BJ, Howard-Ashby M, Nebert DW, Scally M, Dean M, Epel D, Hahn ME, Stegeman JJ (2006) The chemical defensome: environmental sensing and response genes in the Strongylocentrotus purpuratus genome. Dev Biol 300:366–384

    CAS  Article  Google Scholar 

  • Hamdoun A, Epel D (2007) Embryo stability and vulnerability in an always changing world. Proc Natl Acad Sci USA 104:1745–1750

    CAS  Article  Google Scholar 

  • Hamdoun AM, Cherr GN, Roepke TA, Epel D (2004) Activation of multidrug efflux transporter activity at fertilization in sea urchin embryos (Strongylocentrotus purpuratus). Dev Biol 276:452–462

    CAS  Article  Google Scholar 

  • Kurelec B (1992) The multixenobiotic resistance mechanism in aquatic organisms. Crit Rev Toxicol 22:23–43

    CAS  Article  Google Scholar 

  • Panten U, Schwanstecher M, Schwanstecher C (1996) Sulfonylurea receptors and mechanism of sulfonylurea action. Exp Clin Endocrinol Diabetes 104:1–9

    CAS  Article  Google Scholar 

  • Sea Urchin Genome Sequencing Consortium (2006) The genome of the sea urchin Strongylocentrotus purpuratus. Science 314:941–952

    Article  Google Scholar 

  • Shipp LE, Hamdoun A (2012) ATP-binding cassette (ABC) transporter expression and localization in sea urchin development. Dev Dyn 241:1111–1124

    CAS  Article  Google Scholar 

  • Smital T, Luckenbach T, Sauerborn R, Hamdoun AM, Vega RL, Epel D (2004) Emerging contaminants – pesticides, PPCPs, microbial degradation products and natural substances as inhibitors of multixenobiotic defense in aquatic organisms. Mutat Res 552:101–117

    CAS  Article  Google Scholar 

  • Szakács G, Váradi A, Özvegy-Laczka C, Sarkadi B (2008) The role of ABC transporters in drug absorption, distribution, metabolism, excretion and toxicity (ADME–Tox). Drug Discov Today 13:379–393

    Article  Google Scholar 

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This work has been supported by the Ministry of Science, Education and Sports of the Republic of Croatia, Project Nos. 058-0582261-2246 and 098-0982934-2745.

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Correspondence to Ivana Bošnjak.

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Bošnjak, I., Zaja, R., Klobučar, R.S. et al. Identification of ABC Transporter Genes in Gonad Tissue of Two Mediterranean Sea Urchin Species: Black, Arbacia lixula L., and Rocky, Paracentrotus lividus L.. Bull Environ Contam Toxicol 91, 415–419 (2013).

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  • ABC transporters
  • MXR mechanism
  • Arbacia lixula
  • Paracentrotus lividus