Abstract
A method was developed using matrix solid-phase dispersion, together with liquid chromatography with ultraviolet diode array detector for determination of carbofuran, difenoconazole, β-cyfluthrin, spirodiclofen and thiophanate-methyl in stem of coconut palm. The best results were obtained using 2.0 g of stem, 1.6 g of Florisil as sorbent and cyclohexane:acetone mixture (4:1). The method was validated using stem samples spiked with pesticides at four concentration levels (0.05–2.0 μg/g). Average recoveries ranged from 70 % to 114.3 %, with relative standard deviations between 1.2 % and 19.2 %. Detection and quantification limits were in the ranges 0.02–0.03 and 0.05–0.1 μg/g, respectively.
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References
Al-Samarrie AI, Akela AA (2011) Distribution of injected pesticides in data palm trees. Agric Biol J N Am 12:1416–1426
Aquino A, Navickiene S (2009) MSPD procedure for determination of carbofuran, pyrimethanil and tetraconazole residues in banana by GC-MS. Chromatographia 70:1265–1269
Aquino A, Bezerra DSS, Navickiene S (2010) A matrix solid-phase dispersion method for the extraction of seven pesticides from mango and papaya. J Chromatogr Sci 48:750–754
Barker SA (2007) Matrix solid-phase dispersion (MSPD). J Biochem Biophys Meth 70:151–162
Bliesner DM (2006) Validating chromatographic methods—a practical guide. Wiley, New Jersey
Capriotti AL, Cavaliere C, Giansanti P, Gubbiotti R (2010) A recent developments in matrix solid-dispersion extraction. J Chromatogr A 1217:2521–2532
Fontes HR, Procopio SO, Cargnelutti Filho A, Ferreira JMS, Fernandes MF (2009) Efficacy of the herbicide MSMA in eradicating coconut trees infected with stem-bleeding. Planta Daninha 27:859–865
Freitas SS, Lanças FM (2009) Matrix effects observed during pesticides residue analysis in fruits by GC. J Sep Sci 32:3698–3705
Fróes MBR, Santos LFS, Navickiene S (2013) Multi-residue determination of pesticides Açaí tropical fruit (Euterpe oleracea) by matrix solid-phase dispersion combined with liquid chromatography. Food Anal Meth 6:328–333
Garcia-Lopes M, Canosa P, Rodrígues J (2008) Trends and recent applications on matrix solid-phase dispersion. Anal Bioanal Chem 391:963–974
Lecomte P, Darrieutort G (2007) Evaluation of trunk injection technique to control grapevine wood deseases. Phytopathol Mediterr 46:50–57
Mohan C, Radhakrishnan Nair CP, Nampoothiri CK, Rajan P (2010) Leaf-eating caterpillar (Opisina arenosella) induced yield loss in coconut palm. Int J Trop Insect Sci 30:132–137
Nollet LML, Rathore HS (2010) Handbook of pesticides-methods of pesticide residues analysis. CRC Press, Boca Raton
Percival GC, Boyle S (2005) Evaluation of microcapsule trunk injections for the control of apple scab and powdery mildew. Ann Appl Biol 147:119–127
Ranasinghe CS, Fernando WPKK, Zoneer SMM, Mubarak HM (2003) Analysis of residue levels in kernel and nut water of coconut palms injected with monocrotophos. J Natl Sci Found Sri 31:431–436
SANCO No.12495/2011 (2012) Method validation and quality control procedures for pesticide residues analysis in food and feed. The European Commission, United Kingdom
Acknowledgments
The authors wish to thank MCTI/CNPq (process number 620247/2008-8) for financial support of this study. JAF also thanks CAPES for provision of a research fellowship.
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Ferreira, J.A., Santos, L.F.S., Souza, N.R.S. et al. MSPD Sample Preparation Approach for Reversed-Phase Liquid Chromatographic Analysis of Pesticide Residues in Stem of Coconut Palm. Bull Environ Contam Toxicol 91, 160–164 (2013). https://doi.org/10.1007/s00128-013-1018-3
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DOI: https://doi.org/10.1007/s00128-013-1018-3