Advertisement

Simultaneous Determination of Spinetoram Residues in Tomato by High Performance Liquid Chromatography Combined with QuEChERS Method

  • Farag Mahmoud Malhat
Article

Abstract

A sensitive and simple method for simultaneous analysis of spinetoram residues and its dissipation in tomato were studied. Spinetoram residues were extracted from tomato samples with acetonitrile. The extract was cleaned-up with QuEChERS method by dispersive solid-phase extraction with primary secondary amine sorbent to remove co-extractives, prior to analysis by high performance liquid chromatography coupled with diode array detector (HPLC–DAD). This method is characterized by recovery >97 %, relative standard division (RSD) <12.3 %, and limit of quantification (LOQ) of 0.04 mg kg−1, in agreement with directive for method validation in residue analysis. Also, the results showed that spinetoram dissipation pattern followed the first order kinetics with the half-life of 2.6 days, in tomato. The spinetoram residues in tomato were below the codex maximum residue level (0.06 mg kg−1) after 10 days of application. This study suggests that spinetoram is acceptable to apply for tomato under the recommended dosage.

Keywords

Spinetoram Residue Dissipation HPLC–DAD 

References

  1. Al-Samariee AI, Shaker KAM, Al-Bassomy MA (1988) Residue levels in three organophosphorus insecticides in sweet pepper grown in commercial greenhouse. Pestic Sci 22:189–194CrossRefGoogle Scholar
  2. Cabras P, Meloni M, Meloni M, Cabitza F, Cubeddu M (1989) Pesticide residues in lettuce. 2. Influence of formulation. J Agric Food Chem 37:1405–1407CrossRefGoogle Scholar
  3. Cabras P, Spanedda L, Cabitza F, Cubeddu M, Martini M, Brandolini G (1990) Pirimicarb and its metabolites residues in lettuce. Influence of cultural environment. J Agric Food Chem 38:879–882CrossRefGoogle Scholar
  4. Codex Alimentarius Commission (2009) Codex maximum residue limits for pesticides. http://apps.fao.org/faostat/collections?version=ext&hasbulk=0&subset=foodQuqlity.FAO/WHO
  5. Gennari M, Zannini E, Cignetti A, Bicchi C, D’Amato A, Taccheo M, Spessotto C, De Paoli M, Flori P (1985) Vinclozolin decay on different grape vines in four different Italian areas. J Agric Food Chem 33:1232–1237CrossRefGoogle Scholar
  6. Kamel A, Qian Y, Kolbe E, Safford C (2010) Development and validation of a multiresidue method for the determination of nionicotinoid and macrocyclic lactone pesticide residues in milk, fruits, and vegetables by ultra-performance liquid chromatography/MS/MS. Int J AOAC 93(2):389–399Google Scholar
  7. Khay S, Choi J, Abd El-Aty M (2008) Dissipation behavior of lufenuron, benzoyphenylurea insecticide, in/on Chines cabbage applied by foliar spraying under greenhouse condition. Bull Environ Contam Toxicol 81:369–372CrossRefGoogle Scholar
  8. Liu X, Abd El-Aty A, Park J, Park J, Cho S, Shin H, Shim J (2010) Determination of spinetoram in leafy vegetable crops using liquid chromatography and confirmation via tandem mass spectrometry. Biomed Chromatogr 25:1099–1106CrossRefGoogle Scholar
  9. Tsakiris I, Toutoudaki M, Nikitovic D, Danis T, Stratis I, Tsatsakis A (2002) Field study for degradation of methyl parathion in apples cultivated with integrated crop management system. Bull Environ Contam Toxicol 69:771–778CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  1. 1.Pesticide Residues and Environmental Pollution Department, Central Agricultural Pesticide LaboratoryAgriculture Research CenterDokki, GizaEgypt

Personalised recommendations