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Haematology and Blood Chemistry Changes in Mice Treated with Terbuthylazine and its Formulation Radazin TZ-50

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Abstract

TBA is an herbicide in general low acute toxicity and placed into a third category of toxicity. The aim of this study was to determine the effect of TBA and its formulation Radazin TZ-50 in doses of ADI values and 1/100 LD 50 on haematological and biochemical blood parameters in mice. The number of leukocytes was significantly decreased (p < 0.05) in all treated groups compared to non-treated mice (8.81 ± 3.23 × 109/L). The lowest value 3.90 ± 0.74 × 109/L was observed in group treated with TBA (1/100 LD 50) followed by TBA (ADI) 4.49 ± 0.98 × 109/L, Radazin TZ-50 (1/100 LD 50) 4.67 ± 1.24 × 109/L and Radazin TZ-50 (ADI) 4.73 ± 1.15 × 109/L. The values of the enzyme AST was increased from 190.00 ± 26.46–270.00 ± 147.30 U/L in serum of all treated groups as compared to non-treated mice (110.00 ± 20.00). LDH values showed significant increase (3236.67 ± 56.86–4054.33.5 ± 837.16 U/L) as compared to non-treated mice (1010.00 ± 222.71 U/L). Total protein value was significantly (p < 0.05) increased in TBA 1/100 LD50 (63.00 ± 7.48 g/L) and Radazin TZ-50 1/100 LD50 (60.00 ± 2.00 g/L) compared to non-treated mice 52.00 ± 4.00 g/L. Increased serum concentrations of urea and creatinine obtained in mice treated with TBA and Radazin TZ-50 indicates a greater degree of dysfunction of the nephron. TBA and its formulation of Radazin TZ-50 in applied doses demonstrate changes in the number of leukocytes and limited hepatotoxic effects.

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Acknowledgments

This study was supported by the Ministry of Science, Education and Sports of the Republic of Croatia (Grants No. 022-0222148-2137 and No. 119-70-1255).

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Correspondence to V. Benković.

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Benković, V., Đikić, D., Grgorinić, T. et al. Haematology and Blood Chemistry Changes in Mice Treated with Terbuthylazine and its Formulation Radazin TZ-50. Bull Environ Contam Toxicol 89, 955–959 (2012). https://doi.org/10.1007/s00128-012-0813-6

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  • DOI: https://doi.org/10.1007/s00128-012-0813-6

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