Abstract
TBA is an herbicide in general low acute toxicity and placed into a third category of toxicity. The aim of this study was to determine the effect of TBA and its formulation Radazin TZ-50 in doses of ADI values and 1/100 LD 50 on haematological and biochemical blood parameters in mice. The number of leukocytes was significantly decreased (p < 0.05) in all treated groups compared to non-treated mice (8.81 ± 3.23 × 109/L). The lowest value 3.90 ± 0.74 × 109/L was observed in group treated with TBA (1/100 LD 50) followed by TBA (ADI) 4.49 ± 0.98 × 109/L, Radazin TZ-50 (1/100 LD 50) 4.67 ± 1.24 × 109/L and Radazin TZ-50 (ADI) 4.73 ± 1.15 × 109/L. The values of the enzyme AST was increased from 190.00 ± 26.46–270.00 ± 147.30 U/L in serum of all treated groups as compared to non-treated mice (110.00 ± 20.00). LDH values showed significant increase (3236.67 ± 56.86–4054.33.5 ± 837.16 U/L) as compared to non-treated mice (1010.00 ± 222.71 U/L). Total protein value was significantly (p < 0.05) increased in TBA 1/100 LD50 (63.00 ± 7.48 g/L) and Radazin TZ-50 1/100 LD50 (60.00 ± 2.00 g/L) compared to non-treated mice 52.00 ± 4.00 g/L. Increased serum concentrations of urea and creatinine obtained in mice treated with TBA and Radazin TZ-50 indicates a greater degree of dysfunction of the nephron. TBA and its formulation of Radazin TZ-50 in applied doses demonstrate changes in the number of leukocytes and limited hepatotoxic effects.
Similar content being viewed by others
References
Andrew DJ (2007) The interpretation of liver enlargement in regulatory pesticide toxicity studies. Foods Food Ingred J Japan 212(6):465–469
Đikić D, Benković V, Horvat-Knežević A, Brozović G, Oršolić N, Springer OP (2009) Subchronic oral exposure to prometryne changes relations of blood biochemistry indicators in mice. Acta Vet Brno 78:243–251
European Commission (EC) (2002) Guidance document on risk assessment for birds and mammals under council directive 91/414/EEC. SANCO/4145/2000. Brussels, Belgium
European Food Safety Authority (EFSA) (2010) Peer Review Report to the conclusion regarding the peer review of the pesticide risk assessment of the active substance terbuthylazine. Parma, Italy
Everds N (2004) Hematology of the mouse. In: Hedrick HJ, Bullock G (eds) The laboratory mouse. Elsevier Academic Press, San Diego, CA, pp 271–286
Lang DH, Rettie AE, Böcker RH (1997) Identification of enzymes involved in the metabolism of atrazine, terbuthylazine, ametryne, and terbutryne in human liver microsomes. Chem Res Toxicol 10(9):1037–1044
Mercadante R, Polledri E, Giavini E, Menegola E, Bertazzi PA, Fustinoni S (2011) TBA in hair as a biomarker of exposure. Toxicol Lett 210(2):169–173
Organization of economic cooperation and developments (OECD) (2002) OECD pesticide assessment and testing. ENV/JM/MONO 19, Paris, France
Otto S, Altissimo L, Zanin G (2007) TBA contamination of the aquifer north of Vicenza (North–East Italy). Environ Sci Pollut Res 14(2):109–113
Tsatsakis AM, Tzatzarakis MN, Tutudaki M, Babatsikou F, Alegakis AK, Koutis C (2008) Assessment of levels of organochlorine pesticides and their metabolites in the hair of a Greek rural human population. Hum Exp Toxicol 27:933–940
U.S. Environmental Protection Agency USEPA (1995) National water quality inventory: 1994 report to congress. EPA 841-R-95-005., Office of Water, Washington, DC
Werner C (1996) Toxicological evaluation of TBA (GS 13 529) Ciba-Geigy document: 1998 guidelines for drinking-water quality, 2nd ed. Addendum to vol 2. Healthcriteria and other supporting information. WHO, Geneva, Switzerland
Acknowledgments
This study was supported by the Ministry of Science, Education and Sports of the Republic of Croatia (Grants No. 022-0222148-2137 and No. 119-70-1255).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Benković, V., Đikić, D., Grgorinić, T. et al. Haematology and Blood Chemistry Changes in Mice Treated with Terbuthylazine and its Formulation Radazin TZ-50. Bull Environ Contam Toxicol 89, 955–959 (2012). https://doi.org/10.1007/s00128-012-0813-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00128-012-0813-6