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Fast Genomic Biomarker Responses of Retene and Pyrene in Liver of Juvenile Rainbow Trout, Oncorhynchus mykiss

Abstract

We studied the transcriptive effects of two PAHs, retene (RET) and pyrene (PYR), in three equimolar sublethal concentrations (0.9–10 μg/L) in the liver of juvenile rainbow trout, Oncorhynchus mykiss. After 24 h of in vivo exposure, expressions of selected genes (CYP1A, Hsp30, Hsp70, Grp78, Sep15, GP1) were analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). These PAHs changed the studied gene transcriptions differently, but not significantly, except for CYP1A, which was induced only by RET. RET induced CYP1A gene expression even at low, environmentally realistic concentrations in the liver of juvenile rainbow trout.

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Acknowledgments

This study was financed by the Academy of Finland (project 7109823). We thank Ms. Mervi Koistinen and M.Sc. Marja Lahti for help with fish experiments, and Dr. Anssi Lensu for the assistance in statistics (University of Jyväskylä). We also thank M.Sc. Yrjö Lankinen and Mr. Jorma Keränen (Hanka-Taimen Ltd., Venekoski) for the fish.

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Correspondence to Kati Räsänen.

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Räsänen, K., Arsiola, T. & Oikari, A. Fast Genomic Biomarker Responses of Retene and Pyrene in Liver of Juvenile Rainbow Trout, Oncorhynchus mykiss . Bull Environ Contam Toxicol 89, 733–738 (2012). https://doi.org/10.1007/s00128-012-0770-0

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Keywords

  • CYP1A
  • Environmental chemicals
  • Toxicity
  • Transcription
  • Oncorhynchus mykiss