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Development of a Method for the Analysis of Four Plant Growth Regulators (PGRs) Residues in Soybean Sprouts and Mung Bean Sprouts by Liquid Chromatography–Tandem Mass Spectrometry


A method has been developed for the simultaneous determination of four plant growth regulators (PGRs) residues in soybean sprouts and mung bean sprouts. The sample preparation procedure was based on a QuEChERS method. The method showed excellent linearity (r2 ≥ 0.9985) and precision (RSDs ≤ 13.0 %). Average recoveries of four PGRs ranged between 74.9 % and 106.3 % at spiking levels 0.05, 0.5 and 1 mg kg−1. The LODs and LOQs were in the ranges of 0.27–9.3 μg kg−1 and 0.90–31 μg kg−1, respectively. The procedure was applied to 18 bean sprout samples, and benzyladenine was found in some of the analyzed samples.

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The authors are grateful for the support from Chinese National Modern Industrial Technology System Foundation, P. R. of China (Project No: CARS-09-G15) and Chinese National Natural Science Foundation Support (Project No: 31171872).

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Correspondence to Canping Pan.

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Zhang, F., Zhao, P., Shan, W. et al. Development of a Method for the Analysis of Four Plant Growth Regulators (PGRs) Residues in Soybean Sprouts and Mung Bean Sprouts by Liquid Chromatography–Tandem Mass Spectrometry. Bull Environ Contam Toxicol 89, 674–679 (2012).

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  • PGRs
  • Bean sprouts
  • Residue
  • LC–MS/MS