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Development of a Method for the Analysis of Four Plant Growth Regulators (PGRs) Residues in Soybean Sprouts and Mung Bean Sprouts by Liquid Chromatography–Tandem Mass Spectrometry

Abstract

A method has been developed for the simultaneous determination of four plant growth regulators (PGRs) residues in soybean sprouts and mung bean sprouts. The sample preparation procedure was based on a QuEChERS method. The method showed excellent linearity (r2 ≥ 0.9985) and precision (RSDs ≤ 13.0 %). Average recoveries of four PGRs ranged between 74.9 % and 106.3 % at spiking levels 0.05, 0.5 and 1 mg kg−1. The LODs and LOQs were in the ranges of 0.27–9.3 μg kg−1 and 0.90–31 μg kg−1, respectively. The procedure was applied to 18 bean sprout samples, and benzyladenine was found in some of the analyzed samples.

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References

  1. Fan SF, Wang XP, Li PW, Zhang Q, Zhang W (2011) Simultaneous determination of 13 phytohormones in oilseed rape tissues by liquid chromatography-electrospray tandem mass spectrometry and the evaluation of the matrix effect. J Sep Sci 34:640–650

    Article  CAS  Google Scholar 

  2. Flores MI, Romero-González R, Frenich AG, Vidal JL (2011) QuEChERS-based extraction procedure for multifamily analysis of phytohormones in vegetables by UHPLC-MS/MS. J Sep Sci 34:1517–1524

    Article  Google Scholar 

  3. Giannarelli S, Muscatello B, Bogani P, Spiriti MM, Buiatti M, Fuoco R (2010) Comparative determination of some phytohormones in wild-type and genetically modified plants by gas chromatography-mass spectrometry and high-performance liquid chromatography-tandem mass spectrometry. Anal Biochem 398:60–68

    Article  CAS  Google Scholar 

  4. Hou S, Zhu J, Ding M, Lv G (2008) Simultaneous determination of gibberellic acid, indole-3-acetic acid and abscisic acid in wheat extracts by solid-phase extraction and liquid chromatography-electrospray tandem mass spectrometry. Talanta 76:798–802

    Article  CAS  Google Scholar 

  5. Hussain A, Krischke M, Roitsch T, Hasnain S (2010) Rapid determination of cytokinins and auxin in cyanobacteria. Curr Microbiol 61:361–369

    Article  CAS  Google Scholar 

  6. López-Carbonell M, Gabasa M, Jáuregui O (2009) Enhanced determination of abscisic acid (ABA) and abscisic acid glucose ester (ABA-GE) in Cistus albidus plants by liquid chromatography-mass spectrometry in tandem mode. Plant Physiol Biochem 47:256–261

    Article  Google Scholar 

  7. Perrine F, Rolfe B, Hynes M, Hocart C (2004) Gas chromatography-mass spectrometry analysis of indoleacetic acid and tryptophan following aqueous chloroformate derivatisation of Rhizobium exudates. Plant Physiol Biochem 42:723–729

    Article  CAS  Google Scholar 

  8. Wu Y, Hu B (2009) Simultaneous determination of several phytohormones in natural coconut juice by hollow fiber-based liquid-liquid-liquid microextraction-high performance liquid chromatography. J Chromatogr A 1216:7657–7663

    Article  CAS  Google Scholar 

  9. Zhang Y, Li Y, Hu Y, Li G, Chen Y (2010) Preparation of magnetic indole-3-acetic acid imprinted polymer beads with 4-vinylpyridine and β-cyclodextrin as binary monomer via microwave heating initiated polymerization and their application to trace analysis of auxins in plant tissues. J Chromatogr A 1217:7337–7344

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors are grateful for the support from Chinese National Modern Industrial Technology System Foundation, P. R. of China (Project No: CARS-09-G15) and Chinese National Natural Science Foundation Support (Project No: 31171872).

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Correspondence to Canping Pan.

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Zhang, F., Zhao, P., Shan, W. et al. Development of a Method for the Analysis of Four Plant Growth Regulators (PGRs) Residues in Soybean Sprouts and Mung Bean Sprouts by Liquid Chromatography–Tandem Mass Spectrometry. Bull Environ Contam Toxicol 89, 674–679 (2012). https://doi.org/10.1007/s00128-012-0739-z

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Keywords

  • PGRs
  • Bean sprouts
  • Residue
  • LC–MS/MS