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Antibiotic and Heavy Metal Resistance in Bacteria Isolated from the Eastern Mediterranean Sea Coast

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Abstract

In this study it aimed to determine the microbial diversity, level of antibiotic resistance patterns and distribution of heavy metal resistance of bacterial isolates from the Eastern Mediterranean Sea coast. The resistance of 255 Gram-negative bacterial isolates to 16 different antibiotics and to 5 heavy metals was investigated. The most common strains isolated from all samples were Citrobacter koseri (9.0 %), Escherichia coli (8.2 %) and Pantoea agglomerans (8.2 %). Our results revealed a high incidence of resistance to ampicillin (74.0 %), streptomycin (70.0 %) and cefazolin (48.3 %). The multiple antibiotic resistance (MAR) index ranged from 0.2 to 0.75. Isolates showed tolerances to different concentrations of heavy metals. Our results show that the Eastern Mediterranean Sea coast has a significant proportion of antibiotic and heavy metal resistant pathogens, or opportunist Gram-negative bacteria, and these bacteria may result in a potential public health hazard.

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References

  • Akinbowale OL, Peng H, Grant P, Barton MD (2007) Antibiotic and heavy metal resistance in motile aeromonads and pseudomonads from rainbow trout (Oncorhynchus mykiss) farms in Australia. Int J of Antimicrob Ag 30:177–182

    Article  CAS  Google Scholar 

  • Altug G, Balkis N (2009) Levels of some toxic elements and frequency of bacterial heavy metal resistance in sediment and sea water. Environ Monit Assess 149:61–69

    Article  CAS  Google Scholar 

  • Ansari MI, Malik A (2007) Biosorption of nickel and cadmium by metal resistant bacterial isolates from agricultural soil irrigated with industrial wastewater. Bioresour Technol 98:3149–3153

    Article  CAS  Google Scholar 

  • APHA (2005) Standard methods for the examination of water and wastewater, 21st edn. American Public Health Association, Washington, DC

    Google Scholar 

  • Baker-Austin C, Wright MS, Stepanauskas R, McArthur JV (2006) Co-selection of antibiotic and metal resistance. Trends Microbiol 14:176–182

    Article  CAS  Google Scholar 

  • Bauer AW, Kirby WW, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by standard single disk method. Am J Clin Pathol 45:493–496

    CAS  Google Scholar 

  • Chelossi E, Vezzuli L, Milano A, Branzoni M, Fabiano M, Riccardi G, Banat IM (2003) Antibiotic resistance of benthic bacteria in fish-farm and control sediments of the Western Mediterranean. Aquaculture 219:83–97

    Article  CAS  Google Scholar 

  • Chitanand MP, Kadam TA, Gyananath G, Totewad ND, Balhal DK (2010) Multiple antibiotic resistance indexing of coliforms to identify high risk contamination sites in aquatic environment. Indian J Microbiol 50:216–220

    Article  Google Scholar 

  • Çoğun HY, Yüzereroğlu TA, Kargin F, Firat Ö (2005) Seasonal variation and tissue distribution of heavy metals in shrimp and fish species from Yumurtalik coasts of Iskenderun Gulf, Mediterranean. Bull Environ Contam Toxicol 75:707–715

    Article  Google Scholar 

  • Gadd GM (1992) Microbial control of heavy metal pollution. In: Fry JC, Gadd GM, Herbert RA, Jones CW, Watson-Craik IA (eds) Microbial control of pollution. Cambridge Press, Cambridge, pp 59–88

    Google Scholar 

  • Gavini F, Mergaert J, Beji A, Mielcarek C, Izard D, Kersters K, De Ley J (1989) Transfer of Enterobacter agglomerans (Beijerinck 1888) Ewing and Fife 1972 to Pantoea gen. nov. as Pantoea agglomerans comb. nov. and description of Pantoea dispersa sp. nov. Int J Syst Bacteriol 39:337–345

    Article  Google Scholar 

  • Hermansson M, Jones GW, Kjelleberg S (1987) Frequency of antibiotic and heavy metal resistance, pigmentation and plasmids in bacteria of the marine air-water interface. Appl Environ Microbiol 53:2338–2342

    CAS  Google Scholar 

  • Jana S, Bhattacharya DN (1988) Effect of heavy metals on growth population of a fecal coliform bacterium Escherichia coli in aquatic environment. Water Air Soil Pollut 38:251–254

    CAS  Google Scholar 

  • Karayakar F, Erdem C, Cicik B (2007) Seasonal variation in copper, zinc, chromium, lead and cadmium levels in hepatopancreas, gill and muscle tissues of mussel Brachidontes pharaonis Fischer, collected along the Mersin coast, Turkey. Bull Environ Contam Toxicol 79:350–355

    Article  CAS  Google Scholar 

  • Kim KR, Owens G, Kwon SI (2011) Occurence and environmental fate of veterinary antibiotics in the terrestrial environment. Water Air Soil Pollut 214:163–174

    Article  CAS  Google Scholar 

  • Krumperman PH (1983) Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods. Appl Environ Microbiol 46:165–170

    CAS  Google Scholar 

  • Lemos ML, Toranzo AE, Barja JL (1985) Modified medium for the oxidation–fermentation test in the identification of marine bacteria. Appl Environ Microbiol 49:1541–1543

    CAS  Google Scholar 

  • Matyar F, Dincer S, Kaya A, Colak O (2004) Prevalence and resistance to antibiotics in Gram negative bacteria isolated from retail fish in Turkey. Ann Microbiol 54:151–160

    CAS  Google Scholar 

  • Matyar F, Kaya A, Dincer S (2008) Antibacterial agents and heavy metal resistance in Gram-negative bacteria isolated from seawater, shrimp and sediment in Iskenderun Bay, Turkey. Sci Total Environ 407:279–285

    Article  CAS  Google Scholar 

  • NCCLS (2002) Performance standards for antimicrobial disk susceptibility tests, 7th edn. Approved standard National Committee for clinical laboratory standards document M2–A7, vol 20. NCCLS, Villanova

    Google Scholar 

  • Nishibuchi M, Kaper JB (1995) Thermostable direct hemolysin gene of Vibrio parahaemolyticus: a virulence gene acquired by a marine bacterium. Infect Immun 63:2093–2099

    CAS  Google Scholar 

  • Pathak SP, Gopal K (2007) Bacterial contamination and antibiotic resistance in fecal coliforms from glacial water runoff. Bull Environ Contam Toxicol 79:163–167

    Article  CAS  Google Scholar 

  • Pontes DS, Pinheiro FA, Lima-Bittencourt CI, Guedes RLM, Cursino L, Barbosa F, Santos FR, Chartone-Souza E, Nascimento AMA (2009) Multiple antimicrobial resistance of Gram-negative bacteria from natural oligotrophic lakes under distinct anthropogenic influence in a tropical region. Microb Ecol 58:762–772

    Article  CAS  Google Scholar 

  • Ruiz GM, Rawlings TK, Dobbs FC, Drake LA, Mullady T, Huq A, Colwell RR (2000) Global spread of microorganisms by ships. Nature 408:49–50

    Article  CAS  Google Scholar 

  • Samonis G, Karageorgopoulos DE, Kofteridis DP, Matthaiou DK, Sidiropoulou V, Maraki S, Falagas ME (2009) Citrobacter infections in a general hospital: characteristics and outcomes. Eur J Clin Microbiol Infect Dis 28:61–68

    Article  CAS  Google Scholar 

  • Schlusener MP, Bester K (2006) Persistence of antibiotics such as macrolides, tiamulin and salinomycin in soil. Environ Pollut 143:565–571

    Article  Google Scholar 

  • Stachowiak M, Clark SE, Templin RE, Baker KH (2010) Tetracycline-resistant Escherichia coli in a small stream receiving fish hatchery effluent. Water Air Soil Pollut 211:251–259

    Article  CAS  Google Scholar 

  • Türkmen A, Türkmen M, Tepe Y, Mazlum Y, Oymael S (2006) Metal concentrations in blue crab (Callinectes sapidus) and mullet (Mugil cephalus) in Iskenderun Bay, Northern East Mediterranean, Turkey. Bull Environ Contam Toxicol 77:186–193

    Article  Google Scholar 

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Acknowledgments

I would like to thank BAPCOM (Cukurova University) for the financial support of this work (Project number: EF2009BAP3).

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  1. Department of Science and Technology Education, Faculty of Education, Cukurova University, Saricam, 01330, Adana, Turkey

    Fatih Matyar

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  1. Fatih Matyar
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Correspondence to Fatih Matyar.

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Matyar, F. Antibiotic and Heavy Metal Resistance in Bacteria Isolated from the Eastern Mediterranean Sea Coast. Bull Environ Contam Toxicol 89, 551–556 (2012). https://doi.org/10.1007/s00128-012-0726-4

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  • DOI: https://doi.org/10.1007/s00128-012-0726-4

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