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Removal of Crude Oil by Microbial Consortium Isolated from Oil-Spilled Area in the Korean Western Coast

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Abstract

The feasibility of using an indigenous microbial consortium for the removal of crude oil from an oil-spilled coastal area was explored with the ultimate aim of applying for bioremediation. Initially, we obtained the microbial consortium TK-2 that catalyzed the dispersion as well as the degradation of crude oil in supplemented sea water. GC and GC-MS were used to evaluate the removal patterns of crude oil during the incubation. The effective removal of crude oil by TK-2 occurred, and above 95 % of all aliphatic and aromatic compounds detected in this work was removed within 30 days of incubation. Two predominant crude oil-grown isolates derived from TK-2 revealed Gram-negative, rod-shaped cells. Both BIOLOG system and 16S rRNA sequencing were conducted to identify the strains, which were assigned to Arthrobacter sp. HK-2 and Pseudoalteromonas sp. HK-3, and registered in GenBank as [FJ477042] and [FJ477041].

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Acknowledgment

This work was supported in part by the Soonchunhyang University Research Fund.

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Authors and Affiliations

  1. Department of Life Science and Biotechnology, Soonchunhyang University, P.O.Box 97, Asan, Chung-Nam, 336-600, Republic of Korea

    Su-Hee Cho & Kye-Heon Oh

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  1. Su-Hee Cho
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  2. Kye-Heon Oh
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Correspondence to Kye-Heon Oh.

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Cho, SH., Oh, KH. Removal of Crude Oil by Microbial Consortium Isolated from Oil-Spilled Area in the Korean Western Coast. Bull Environ Contam Toxicol 89, 680–685 (2012). https://doi.org/10.1007/s00128-012-0723-7

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  • DOI: https://doi.org/10.1007/s00128-012-0723-7

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