Glutathione-S-Transferase (GST) and metallothionein are important biomarker endpoints in studying the effect of Cd exposure. The purpose of this research was to study the correlation between hepatic GST and metallothionein with hepatic Cd in wild Fejervarya limnocharis exposed to environmental Cd. Results showed that frogs from contaminated sites had significantly higher hepatic metallothionein (3.58 mg/kg wet weight) and GST activity (0.259 μmol/min/mg total protein) than those from the reference site (2.36 mg/kg wet weight and 0.157 μmol/min/mg total protein respectively). There was a significantly positive correlation between hepatic Cd and GST activity (r = 0.802, p = 0.009) but not between hepatic Cd and metallothionein (r = 0.548, p = 0.139). The results concluded that while frogs from the contaminated site had higher GST and metallothionein, only GST showed significant positive correlation with hepatic Cd levels, indicating that hepatic GST activity may be used as a biomarker endpoint.
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This study was supported by the National Center of Excellence for Environmental and Hazardous Waste Management (NCE-EHWM), the 90th Anniversary of Chulalongkorn University Fund, a new staff development grant (Ratchadaphiseksomphot Endowment Fund), and the MUA-TRF research grant (MRG4980120) to NK. An educational grant from the Malaysian Ministry of Higher Education to MSO is fully acknowledged. Additional support was also obtained from NIH Fogarty ITREOH NIH FIC D43TW007849, NIEHS P30ES005022 and NIEHS P32ES007148.
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Othman, M.S., Khonsue, W., Kitana, J. et al. Hepatic Metallothionein and Glutathione-S-Transferase Responses in Two Populations of Rice Frogs, Fejervarya limnocharis, Naturally Exposed to Different Environmental Cadmium Levels. Bull Environ Contam Toxicol 89, 225–228 (2012). https://doi.org/10.1007/s00128-012-0708-6
- Fejervarya limnocharis