Skip to main content

Advertisement

Log in

Biodegradation of Oil Spill by Petroleum Refineries Using Consortia of Novel Bacterial Strains

  • Published:
Bulletin of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

Feasibility study carried out at the site prior to the full scale study showed that the introduced bacterial consortium effectively adapted to the local environment of the soil at bioremediation site. The soil samples were collected from the contaminated fields after treatment with bacterial consortium at different time intervals and analyzed by gas chromatography after extraction with hexane and toluene. At time zero (just before initiation of bioremediation), the concentration of total petroleum hydrocarbons in the soil (25-cm horizon) of plot A, B, C and D was 30.90 %, 18.80 %, 25.90 % and 29.90 % respectively, after 360 days of treatment with microbial consortia was reduced to 0.97 %, 1.0 %, 1.0 %, and 1.1 % respectively. Whereas, only 5 % degradation was observed in the control plot after 365 days (microbial consortium not applied).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Bajpai U, Kuhad RC, Khanna S (1998) Mineralization of [C14] octadecane by Acinetobacter calcoaceticus S19. Can J Microbiol 44:681–686

    CAS  Google Scholar 

  • Banwari L, Khanna S (1996a) Mineralizatin of [14C] Octacosane by Acinetobacter calcoaceticus S30. Can J Microbiol 42:1123–1225

    Google Scholar 

  • Banwari L, Khanna S (1996b) Degradation of crude oil by Acinetobacter calcoaceticus and Alcaligenes odoran. J Appl Bacteriol 81:355–362

    Google Scholar 

  • Cookson JT (1995) Bioremediation engineering: design and application. McGraw-Hill, New York

    Google Scholar 

  • Das K, Mukherjee AK (2007) Crude petroleum-oil biodegradation efficiency of Bacillus subtilis and Pseudomonas aeruginosa strains isolated from a petroleum-oil contaminated soil from North-East India. Bioresour Technol 98:1339–1345

    Article  CAS  Google Scholar 

  • Eikania MH, Golmohamad F, Homani SS (2011) Extraction of pomegranate (Punica granatum L.) seed oil using superheated hexane. Food Bioproduct Process 90:32–36

    Google Scholar 

  • Luque de Castro MD, Priego-Capote F (2010) Soxhlet extraction: past and present panacea. J Chromatogr A 1217:2383–2389

    Article  CAS  Google Scholar 

  • Makadia TH, Adetutu KL, Simson D, Jardien D, Sheppard PJ, Ball AS (2011) Re-use of remediated soils for the bioremediation of waste oil sludge. J Environ Manag 92:866–871

    Article  CAS  Google Scholar 

  • Mishra S, Jyot J, Kuhad RC, Lal B (2001) In situ bioremediation potential of an oily sludge—degrading bacterial consortium. Curr Microbiol 43:328–335

    Article  CAS  Google Scholar 

  • United States Environment Protection Agency 2010. http://www.clu-in.org/

  • Unterbrunner R, Wieshammer G, Hollender U, Felderer B, Wieshammer-Zivkovic M, Puschenreiter Wenzel WW (2007) Plant and fertilizer effects on rhizodegradation of crude oil in two soils with different nutrient status. Plant Soil 300:117–126

    Article  CAS  Google Scholar 

  • Varanasi P, Fullana A, Sidhu S (2007) Remediation of PCB contaminated soils using iron nano-particles. Chemosphere 66:1031–1038

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We are thankful to Dr. R.K. Pachauri, Director General, and Teri for providing the infrastructure to carry out the present study. Thanks to the management of Ahmedabad refinery (Oil and Natural gas Company) where the study has been carried out.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Bina Singh.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Singh, B., Bhattacharya, A., Channashettar, V.A. et al. Biodegradation of Oil Spill by Petroleum Refineries Using Consortia of Novel Bacterial Strains. Bull Environ Contam Toxicol 89, 257–262 (2012). https://doi.org/10.1007/s00128-012-0668-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00128-012-0668-x

Keywords

Navigation