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Multiple In Vitro Bioassay Approach in Sediment Toxicity Evaluation: Masan Bay, Korea

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Abstract

Extracts of 21 sediment samples from Masan Bay, Korea, used in an earlier chemical measurement, were screened for their ability to induce estrogen, – and dioxin – like gene expression using the E-Assay (+), DR-CALUX assay, respectively, and to inhibit acetylcholinesterase (AChE) activity using an in vitro AChE assay. Biological impact in the industry-rich inner bay is higher than outer bay. DDTs (0.65), coplanar PCBs (0.77), HCHs (0.64), PAHs (0.61) and APs (0.53) with good correlation to E-assay (+) are seen as environmental estrogens. The highest induction of DR-CALUX response was seen again at station M12 and 15 which received sewage effluents. PCDD/DFs gave the highest correlation (0.75). Interestingly, the M12 station at the sewage treatment outlet showed the highest activity. Among the targeted chemicals APs (0.66), PCBs (0.64), PAHs (0.61) and DDT (0.49) correlated well with the AChE bioassay. Spearman rank correlation on analytical and biochemical results affirmed the ‘hot spots’ and point sources (e.g., sewage treatment and industrial outfall) and suspected toxicants. Significant correlations between organo chlorine pesticides, PCBs, dioxins and alkylphenols and their biological effects were observed.

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References

  • Bocquené BC, Cadiou Y, Galgani F (1995) Joint action of combinations of pollutants on the acetylcholinesterase activity of several marine species. Ecotoxicology 4:266–279

    Article  Google Scholar 

  • Chuiko GM, Tillitt DE, Zajicek JL, Flerov BA, Stepanova VM, Zhelnin YY, Podgornaya VA (2007) Chemical contamination of the Rybinsk Reservoir, northwest Russia: relationship between liver polychlorinated biphenyls (PCBs) content and health indicators in bream (Abramis brama). Chemosphere 67:527–536

    Article  CAS  Google Scholar 

  • Clemons JH, Allan LM, Marvin CH, Wu Z, Mccarry BE, Bryant DW, Zacharewski TR (1998) Evidence of estrogen- and TCDD-like activities in crude and fractionated extracts of PM10 air particulate material using in vitro gene expression assays. Environ Sci Technol 32:1853–1860

    Google Scholar 

  • Ellman GL, Courtney KD, Andres JV, Featherstone RM (1961) A new and rapid 271 colorimetric determination of acetylcholinesterase activity. Biochemical 272 Pharmacol 7:88–95

    Google Scholar 

  • Giacobini E (2000) Cholinesterase inhibitors: from the Calabar bean to Alzheimer therapy. In: Giacobini E (ed) Cholinesterases and cholinesterase inhibitors. Martin Dunitz, London, pp 181–227

  • Glusczak L, Miron DS, Crestani M, Fonseca, Pedron FA, Duarte MF, Vieir VLP (2006) Effect of glyphosate herbicide on acetylcholinesterase activity and metabolic and hematological parameters in piava (Leporinus obtusidens). Ecotoxicol Environ Saf 65:237–241

  • Gozgit JM, Nestor KM, Fasco MJ, Pentecost BT, Arcaro KF (2004) Differential action of polycyclic aromatic hydrocarbons on endogenous estrogen-responsive genes and on a transfected estrogen-responsive reporter in MCF-7 cells. Toxicol Appl Pharmacol 196:58–67

    Article  CAS  Google Scholar 

  • Hamblen EL, Cronin MT, Schultz TW (2003) Estrogenicity and acute toxicity of selected anilines using a recombinant yeast assay. Chemosphere 52:1173–1181

    Article  CAS  Google Scholar 

  • Hamers T, Leonards PEG, Legler J, Vethaak AD, Schipper CA (2010) Toxicity profiling: an integrated effect-based tool for site-specific sediment quality assessment. Integr Environ Assess Manag 6:761–773

    Article  CAS  Google Scholar 

  • Hong SH, Yim UH, Shim WJ, Oh JR, Lee IS (2003) Horizontal and vertical distribution of PCBs and chlorinated pesticides in sediments from Masan Bay, Korea. Mar Pollut Bull 46:244–253

    Article  CAS  Google Scholar 

  • Hong SH, Munschy C, Kannan N, Tixier C, Tronczynski J, Héas-Moisan K, Shim WJ (2009) PCDD/F, PBDE, and nonylphenol contamination in a semi-enclosed bay (Masan Bay, South Korea) and a Mediterranean Lagoon (Thau, France). Chemosphere 77:854–862

    Article  CAS  Google Scholar 

  • Hong SH, Kannan N, Jin YN, Shim WJ (2010) Temporal trend, spatial distribution, and terrestrial sources of PBDEs and PCBs in Masan Bay, Korea. Mar Pollut Bull 60:1836–1841

    Article  CAS  Google Scholar 

  • Jung JH, Addison RF, Shim WJ (2007) Characterization of cholinesterases in marbled sole, Limanda yokohamae, and their inhibition in vitro by the fungicide iprobenfos. Mar Environ Res 63:471–478

    Article  CAS  Google Scholar 

  • Jung JH, Kim SJ, Lee TK, Shim WJ, Woo S, Kim DJ, Han CH (2008) Biomarker responses in caged rockfish (Sebastes schlegeli) from Masan Bay and Haegeumgang, South Korea. Mar Pollut Bull 57:599–606

    Article  CAS  Google Scholar 

  • Kannan N, Hong SH, Shim WJ, Oh JR (2007) A congener-specific survey for polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) contamination in Masan Bay, Korea. Chemosphere 68:1613–1622

    Article  CAS  Google Scholar 

  • Kannan N, Hong SH, Yim UH, Kim NS, Ha SY, Li D, Shim WJ (2010) Dispersion of organic contaminants from wastewater treatment outfall in Masan Bay, Korea. Toxicol Environ Health Sci 2:200–206

    Article  Google Scholar 

  • Kim NS, Ha SY, An JG, Yim UH, Hong SH, Kim M, Kannan N, Shim WJ (2011) Status and trend of butyltin contamination in Masan Bay, Korea. Toxicol Environ Health Sci 3:46–53

    Article  Google Scholar 

  • Lee HJ, Hong SH, Kim M, Ha SY, An SM, Shim WJ (2011) Tracing origins of sewage and organic matter using dissolved sterols in Masan and Haengam Bay, Korea. Ocean Sci J 46(2):95–103

    Article  CAS  Google Scholar 

  • Li MH (2008) Effects of nonylphenol on cholinesterase and carboxylesterase activities in male guppies (Poecilia reticulata). Ecotoxicol Environ Saf 71:781–786

    Article  CAS  Google Scholar 

  • Li DH, Dong M, Shim WJ, Yim UH, Hong SH, Kannan N (2008) Distribution characteristics of nonylphenolic chemicals in Masan Bay environments, Korea. Chemosphere 71:1162–1172

    Article  CAS  Google Scholar 

  • Menéndez-Helman RJ, Ferreyroa GV, dos Santos Afonso M, Salibián A (2012) Glyphosate as an acetylcholinesterase inhibitor in Cnesterodon decemmaculatus. Bull Environ Contam Toxicol 88(1):6–9

    Article  Google Scholar 

  • Mora P, Fournier D, Narbonne JF (1999) Cholinesterase from the marine mussels Mytilus galloprovincialis L. and Mytilus edulus L. from the freshwater bivalve Corbicula fluminea Muller. Comp Biochem Physiol Part C 122:353–361

    CAS  Google Scholar 

  • Moreira SM, Guilhermino L (2005) The use of the Mytilus galloprovincialis acetylcholinesterase and glutathione S-transferases activities as biomarkers of environmental contamination along the North West Portuguese coast. Environ Monit Assess 105:309–325

    Article  CAS  Google Scholar 

  • Moreira SM, Moreira-Santos M, Ribeiro R, Guilhermino L (2004) The “Coral Bulker” fuel oil spill on the north coast of Portugal: spatial and temporal biomarker responses in Mytilus galloprovincialis. Ecotoxicology 13:619–630

    Article  CAS  Google Scholar 

  • Nishikawa JI, Saito K, Goto J, Dakeyama F, Matsuo M, Nishhara T (1999) New screening methods for chemicals with hormonal activity using interaction of nuclear hormone receptor with coactivator. Toxicol Appl Pharmacol 154:76–83

    Article  CAS  Google Scholar 

  • Payne JF, Mathieu A, Melvin W, Fancey LL (1996) Acetylcholinesterase, an old biomarker with a new future: field trials in association with two urban rivers and a paper mill in Newfoundland. Mar Pollut Bull 32:225–231

    Article  CAS  Google Scholar 

  • Safe S (1990) Polychlorinated biphenyls (PCBs), dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), and related compounds: environmental and mechanistic considerations which support the development of toxic equivalency factors (TEFs). Crit Rev Toxicol 21:51–88

    Article  CAS  Google Scholar 

  • Sancho E, Fernandez-Vega C, Sanchez M, Ferrando MD, Andreu-Moliner E (2000) Alterations on AChE activity of the fish Anguilla anguilla as response to herbicide-contaminated water. Ecotoxicol Environ Safety 46:57–63

    Article  CAS  Google Scholar 

  • Shim WJ, Oh JR, Kahng SH, Shim JH, Lee SH (1999) Horizontal distribution of butyltins in surface sediments from an enclosed bay system, Korea. Environ Pollut 106:351–357

    Article  CAS  Google Scholar 

  • Soto AM, Chung KL, Sonnenschein C (1994) The pesticides endosulfan, toxaphene, and dieldrin have estrogenic effects on human estrogen-sensitive cells. Environ Health Perspect 102:380–383

    Article  CAS  Google Scholar 

  • Talorete TPN, Isoda H, Maekawa T (2001) Alkylphenolic compounds and their effect on the injury rate, survival and acetylcholinesterase activity of the rat neuronal cell line PC12. Cytotechnology 36:163–169

    Article  CAS  Google Scholar 

  • Vakharia D, Gierthy J (1999) Rapid assay for oestrogen receptor binding to PCB metabolites. Toxicol In Vitro 13(2):275–282

    Article  CAS  Google Scholar 

  • Wandji SA, Gandhi R, Snedeker SM (1998) Critical evaluation of chlordane’s breast cancer risk. Critical evaluation # 4. Cornell University Program on Breast Cancer and Environmental Risk Factors in New York State (BCERF)

  • Yim UH, Hong SH, Shim WJ, Oh JR, Chang M (2005) Spatio-temporal distribution and characteristics of PAHs in sediments from Masan Bay, Korea. Marine Pollut Bull 50:319–326

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by a grant (PE98745) from Korea Ocean Research and Development Institute.

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Authors and Affiliations

  1. Oil and POPs Research Group, Korean Ocean Research and Development Institute, 391 Jangmok Myon, Geoje, Korea

    Jee-Hyun Jung, Sang Hee Hong, Un Hyuk Yim, Sung Yong Ha, Won Joon Shim & N. Kannan

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  1. Jee-Hyun Jung
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  2. Sang Hee Hong
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  3. Un Hyuk Yim
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  4. Sung Yong Ha
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  5. Won Joon Shim
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  6. N. Kannan
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Correspondence to N. Kannan.

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Jung, JH., Hong, S.H., Yim, U.H. et al. Multiple In Vitro Bioassay Approach in Sediment Toxicity Evaluation: Masan Bay, Korea. Bull Environ Contam Toxicol 89, 32–37 (2012). https://doi.org/10.1007/s00128-012-0656-1

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  • DOI: https://doi.org/10.1007/s00128-012-0656-1

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