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Chromium (VI) Reduction by Cell Free Extract of Ochrobactrum anthropi Isolated from Tannery Effluent

Abstract

Chromium-resistant bacteria isolated from industrial wastes can be used to detoxify toxic chromium from contaminated sources. From effluent of Shafiq Tannery, Kasur, Pakistan, bacterial strain STCr-1 that could endure 40 mg mL−1 of potassium chromate in nutrient agar medium was isolated. STCr-1, identified as Ochrobactrum anthropi by 16S rRNA gene sequence homology, demonstrated substantial Cr(VI) reduction at pH 7 and temperature 37°C. It completely reduced 250 μg mL−1 of Cr(VI) and showed 71.2 % Cr(VI) reduction at Cr(VI) concentrations of 550 μg mL−1. Rate of Cr(VI) reduction increased with increase in cell and Cr(VI) concentration. The presence of Cu2+, Co2+ and Mn2+ significantly stimulated Cr(VI) reduction. Assay with cell free extracts clearly indicated that Cr(VI) reduction was solely associated with the soluble fraction of the cell.

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Correspondence to Sikander Sultan.

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Sultan, S., Hasnain, S. Chromium (VI) Reduction by Cell Free Extract of Ochrobactrum anthropi Isolated from Tannery Effluent. Bull Environ Contam Toxicol 89, 152–157 (2012). https://doi.org/10.1007/s00128-012-0648-1

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Keywords

  • Cr(VI)-resistance
  • Tannery effluent
  • Ochrobactrum anthropi
  • Cr(VI) reduction
  • Cell free extract