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Comet Assay to Assess the Genotoxicity of Persian Walnut (Juglans regia L.) Husks with Statistical Evaluation


The aim of this study was to confirm the utility of the Comet assay as a genotoxicity screening test for evaluating the impact of walnut husk aqueous extract. Phytotoxicity assays using diluted and undiluted walnut husk aqueous extracts were performed on young roots of Raphanus sativus (radish), and the Comet assay was used to evaluate DNA integrity in isolated radish radicle nuclei. The results reveal a dose-dependent accumulation of DNA damage in radish radicles treated with walnut husks water extract and that the Kolmogorov-Smirnov test combined with Johnson SB distribution was the best approach for describing Comet assay data.

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The authors wish to thank Prof. G. Aliotta (Second University of Naples, Italy) for insightful discussion. The authors also acknowledge financial support of Project BIONUTS, from the Ministero delle Politiche Agricole Alimentari e Forestali (D.M. no. 24322/7742/09 22/10/2009). The authors declared no potential conflicts of interest with respect to the authorship and publication of this article.

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Correspondence to Milena Petriccione.

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Petriccione, M., Ciniglia, C. Comet Assay to Assess the Genotoxicity of Persian Walnut (Juglans regia L.) Husks with Statistical Evaluation. Bull Environ Contam Toxicol 89, 166–171 (2012).

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  • Persian walnut
  • Comet assay
  • Kolmogorov-Smirnov test
  • Genotoxicity