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The Acute and Behavioral Effects of a Copper-Nickel Mixture on Roach Rutilus rutilus


Semi-static acute toxicity tests were conducted on adult roach, Rutilus rutilus, to estimate its sensitivity toward an equitoxic binary mixture (EBM) of copper and nickel. The sum of their individual LC50 values was considered to equal 100 %. The main endpoints of the study were mortality and behavioral responses: detection, locomotor activity, coughing rate and pectoral-fin activity. The 96-h LC50 of EBM was 14.4 (10.1 %–20.5 %), indicating a synergism of individual metals. The most meaningful behavioral results were obtained after 10-min, 1-h and 24-h exposures, and the most sensitive and informative behavioral response was found to be coughing rate. This bioassay response may be used successfully to evaluate wastewaters containing heavy metals for their toxicity toward fish.

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  1. Alabaster JS, Lloyd R (1982) Water quality criteria for freshwater fish. FAO and Butterworths, London

    Google Scholar 

  2. ASTM (2008a) Standard guide for measurement of behavior during fish toxicity tests. E1711-95(2008). ASTM International, West Conshohocken, PA

  3. ASTM (2008b) Standard guide for ventilatory behavioral toxicology testing of freshwater fish. E1768-95(2008). ASTM International, West Conshohocken, PA

  4. Atchison GJ, Henry MG, Sandheinrich MB (1987) Effects of metals on fish behavior: a review. Environ Biol Fish 18:11–25

    Article  Google Scholar 

  5. De Boeck G, Vlaeminck A, Balm PH, Lock RA, De Wachter B, Blust R (2001) Morphological and metabolic changes in common carp, Cyprinus carpio, during short-term copper exposure: interactions between Cu2+ and plasma cortisol elevation. Environ Toxicol Chem 20:374–381

    Google Scholar 

  6. Drummond RA, Spoor WA, Olson GF (1973) Some short-term indicators of sublethal effects of copper on brook trout, Salvelinus fontinalis. J Fish Res Board Can 30:698–701

    Article  CAS  Google Scholar 

  7. Eisler R. (1998a) Copper hazards to fish, wildlife and invertebrates: a synoptic review. Biological science report USGS/BRD/BSRN-1997-0002, Washington, DC

  8. Eisler R (1998b) Nickel hazards to fish, wildlife and invertebrates: a synoptic review. Biological science report USGS/BRD/BSR-1998-0001, Laurel, MD

  9. Ellgaard EG, Ashley SE, Langford AE, Harlin DC (1995) Kinetic analysis of the swimming behavior of the goldfish Carassius auratus exposed to nickel: hypoactivity induced by sublethal concentrations. Bull Environ Contam Toxicol 55:929–936

    Article  CAS  Google Scholar 

  10. Flerov BA (1989) Ecological and physiological aspects of toxicology in fresh-water animals (in Russian). Nauka, Leningrad

    Google Scholar 

  11. Gagnon A, Jumarie C, Hontela A (2006) Effects of Cu on plasma cortisol and cortisol secretion by adrenocortical cells of rainbow trout Oncorhynchus mykiss. Aquat Toxicol 78:59–65

    Article  CAS  Google Scholar 

  12. Hamilton MA, Russo RC, Thurston RV (1977) Trimmed Spearman-Karber method for estimating median lethal concentrations in toxicity bioassays. Environ Sci Technol 11:714–719

    Article  CAS  Google Scholar 

  13. Henry MG, Atchison GJ (1986) Behavioral changes in social groups of bluegills exposed to copper. Trans Am Fish Soc 115:590–595

    Article  Google Scholar 

  14. Kane AS, Salierno JD, Brewer SK (2005) Fish models in behavioral toxicology: automated techniques, updates and perspectives. In: Ostrander GK (ed) Methods in aquatic toxicology, 2nd edn. Lewis Publishers, Boca Raton, pp 559–590

    Google Scholar 

  15. Kazlauskienė N, Vosylienė MZ (1990) Effect of copper on respiratory system of rainbow trout (in Russian). In: Andrušaitis GP et al. (eds) Experimental aquatic toxicology, vol 14. Zinatne, Riga, pp 136–144

  16. Könemann H (1981) Fish toxicity tests with mixtures of more than two chemicals: a proposal for a quantitative approach and experimental results. Toxicology 19:229–238

    Article  Google Scholar 

  17. Lemly AD (1983) A simple activity quotient for detecting pollution-induced stress in fishes. Environ Tech Lett 4:173–178

    Article  CAS  Google Scholar 

  18. Little EE, Finger SE (1990) Swimming behavior as an indicator of sublethal toxicity in fish. Environ Toxicol Chem 9:13–19

    Article  CAS  Google Scholar 

  19. Scherer E (1992) Behavioural responses as indicators of environmental alterations: approaches, results, developments. J Appl Ichthyol 8:122–131

    Article  Google Scholar 

  20. Scherer E, Harrison SE, Brown SB (1986) Locomotor activity and blood plasma parameters of acid-exposed lake whitefish Coregonus clupeaformis. Canadian J Fish Aquat Sci 43:1556–1561

    Article  CAS  Google Scholar 

  21. SCORECARD (2005) Water quality indicators.

  22. Svecevičius G (2005) Behavioral responses of rainbow trout Oncorhynchus mykiss to sublethal toxicity of a model mixture of heavy metals. Bull Environ Contam Toxicol 74:845–852

    Article  Google Scholar 

  23. Svecevičius G (2010) Acute toxicity of nickel to five species of freshwater fish. Pol J Environ Stud 19:453–456

    Google Scholar 

  24. Svecevičius G, Vosylienė MZ (1996) Acute toxicity of copper to common fishes of Lithuania. Ekologija 2:17–21

    Google Scholar 

  25. Tudorache C, Jordan AD, Svendsen JC et al (2009) Pectoral fin beat frequency predicts oxygen consumption during spontaneous activity in a labriform swimming fish Embiotoca lateralis. Environ Biol Fish 84:121–127

    Article  Google Scholar 

  26. US EPA (2002) Short-term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms. 4th edn. EPA-821-R-02-013. Office of Water. Washington, DC

  27. US EPA (2006) National recommended water quality criteria. Office of Water. Office of Science and Technology (4304T). Washington, DC

  28. US EPA (2007) Aquatic life ambient freshwater quality criteria—copper. 2007 Revision. EPA-822-R-07-001. Office of Water. Office of Science and Technology. Washington, DC

  29. US EPA (1984) Ambient water quality criteria for copper—EPA-440/5-84-031. Office of Water Regulations and Standards, Washington, DC

    Google Scholar 

  30. USEPA (1986) Ambient water quality criteria for nickel—EPA-440/5-86-004. Office of Water Regulations and Standards, Washington, DC

    Google Scholar 

  31. Van der Schalie WH, Shedd TR, Knechtges PL, Widder MW (2001) Using higher organisms in biological early warning systems for real-time toxicity detection. Biosens Bioelectron 16:457–465

    Article  Google Scholar 

  32. Waser W, Bausheva O, Nikinmaa M (2009) The copper-induced reduction of critical swimming speed in rainbow trout Oncorhynchus mykiss is not caused by changes in gill structure. Aquat Toxicol 94:77–79

    Article  CAS  Google Scholar 

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This work was funded by Research Council of Lithuania, Project No. MIP-58/2010-2011.

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Correspondence to Gintaras Svecevičius.

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Svecevičius, G., Kazlauskienė, N. & Taujanskis, E. The Acute and Behavioral Effects of a Copper-Nickel Mixture on Roach Rutilus rutilus . Bull Environ Contam Toxicol 89, 147–151 (2012).

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  • Fish
  • Roach
  • Acute toxicity
  • Behavior
  • Copper-nickel equitoxic binary mixture
  • Synergism