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Direct Estimation of Carbaryl by Gas Liquid Chromatography with Nitrogen Phosphorus Detection

Abstract

A simple and efficient analytical method was standardized for the estimation of residues of carbaryl in various substrates comprising grape berries, kinnow pulps, kinnow rind and soil. The samples were refluxed using mixture of methanol: 0.5 N HCl (1:1 v/v); diluted with brine solution, partitioned into chloroform and dried over anhydrous sodium sulfate. Further the samples were treated with anhydrous magnesium sulfate and primary secondary amine. Final clear extracts were concentrated under vacuum and reconstituted the volume into acetone. The residues were estimated directly on gas liquid chromatograph equipped with nitrogen phosphorus detection system equipped with a capillary column packed with 5 % diphenyl 95 % dimethyl polysiloxane non-polar phase. A consistent recovery from 82 % to 97 % for carbaryl was observed when samples were spiked at levels ranging from 0.05 to 1.00 mg kg−1. The limit of quantification of the method was worked out to be 0.05 mg kg−1 for grape berries, kinnow pulp, kinnow rind and soil.

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Acknowledgments

The authors are thankful to the professor and Head, Department of Entomology, PAU, Ludhiana for providing the necessary research facilities. Financial assistance provided by Indian Council of Agricultural Research (ICAR), New Delhi is also gratefully acknowledged.

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Correspondence to Raminderjit Singh Battu.

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Battu, R.S., Mandal, K., Urvashi et al. Direct Estimation of Carbaryl by Gas Liquid Chromatography with Nitrogen Phosphorus Detection. Bull Environ Contam Toxicol 89, 15–20 (2012). https://doi.org/10.1007/s00128-012-0624-9

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Keywords

  • Carbaryl
  • Grape berries
  • Kinnow pulp
  • Kinnow rind
  • Soil