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Histological Analysis of the Impact of Lagoon Pollution on Chrysichthys nigrodigitatus from Cote d’Ivoire

Abstract

Immunohistological and histopathological methods were used to highlight the importance of cell damages and some biomarkers for health risk assessment. A comparative study between 3 sites of the lagoons showed that the most polluted areas (Adiake and Ebrah), influenced by human activities, presented more damaged cells and stained cells in gills and livers of Chrysichthys nigrodigitatus than the less polluted area (Layo): gill cell hyperplasia and liver cells vacuolation were more evident in fish from Adiake than in fish from Ebrah and Layo. The percentage of proliferating cell nuclear antigen (PCNA)-positive cells in gill were 45.8 % ± 23.7 % for Adiake, 18.2 % ± 4.6 % for Ebrah and 11.4 % ± 6.51 % for Layo; The percentage of PCNA-positive in liver cells were 3.8 % ± 3.6 % for Adiake, 4.9 % ± 4.7 % for Ebrah and 2.6 % ± 2.5 % for Layo. Gills were more affected than livers. The Adiake site was the most contaminated area of the lagoon complex of Cote d’Ivoire.

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Acknowledgments

This research was funded with a grant from the Fulbright Scholars Program (Advanced Research and University lecturing Awards). We thank the Williams Laboratory at Oregon State University (OSU) and the team at the Veterinary Diagnostic Lab at OSU, especially Kay Fisher and Christiane Löhr, for their continuous support and expertise. We are grateful to Gayle Orner, Lyndsey Shorey and Sharon Krueger for help in the lab.

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Correspondence to Abiba Sanogo Tidou.

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Tidou, A.S., Yapi, Ac.D., Kouamé, V.K. et al. Histological Analysis of the Impact of Lagoon Pollution on Chrysichthys nigrodigitatus from Cote d’Ivoire. Bull Environ Contam Toxicol 89, 44–52 (2012). https://doi.org/10.1007/s00128-012-0617-8

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Keywords

  • PCNA
  • Immunohistochemistry
  • Fish tissue damages
  • Lagoon Cote d’Ivoire