Skip to main content

Advertisement

Springer Nature Link
Log in

Biochemical Changes in Pig Serum After Ochratoxin A Exposure

  • Published:
Bulletin of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

The objective of this study was to investigate the effect of ochratoxin A (OTA) on serum biochemical parameters of pigs during subchronic treatment with 300 μg OTA/kg of feed for 30 days. OTA treatment resulted in significantly higher (p < 0.05) serum levels of creatinine, urea, potassium and alkaline phosphatase, and significantly lower levels of glucose and total protein. These changes in serum biochemical parameters in treated pigs were indicative of impaired liver and kidney function caused by OTA exposure.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

References

  • Anzai N, Jutabha P, Endou H (2010) Molecular mechanism of ochratoxin A transport in kidney. Toxins 2:1381–1398

    Article  CAS  Google Scholar 

  • Denli M, Perez JF (2010) Ochratoxins in feed, a risk for animal and human health: control strategies. Toxins 2:1065–1077

    Article  CAS  Google Scholar 

  • Dietrich DR, Heussner AH, O’Brein E (2005) Ochratoxin A: comparative pharmacokinetics and toxicological implications (experimental and domestic animals and humans). Food Addit Contam 22(Suppl 1):45–52

    Article  CAS  Google Scholar 

  • Domijan A-M, Peraica M, Cvjetković B, Turčin S, Jurjević Ž, Ivić D (2005) Mould contamination and co-occurrence of mycotoxins in maize grain in Croatia. Acta Pharm 55:349–356

    CAS  Google Scholar 

  • Elaroussi MA, Mohamed FR, Elgendy MS, El Barkouky EM, Abdou AM, Hatab MH (2008) Ochratoxicosis in broiler chickens: functional and histological changes in target organs. Int J Poult Sci 7:414–422

    Article  CAS  Google Scholar 

  • Finco DR (1980) Kidney function. In: Kaneko JJ (ed) Clinical biochemistry of domestic animals, 3rd edn. Academic Press, New York, NY, pp 338–400

    Google Scholar 

  • Fuchs R, Hult K (1992) Ochratoxin A in blood and its pharmacokinetics properties. Food Chem Toxicol 30:201–204

    Article  CAS  Google Scholar 

  • Galtier P, Alvinerie M, Charpenteau JL (1981) The pharmacokinetics profiles of ochratoxin A in pigs, rabbits and chickens. Food Cosmet Toxicol 19:735–738

    Article  CAS  Google Scholar 

  • Gareis M, Scheuer R (2000) Ochratoxin A in meat and meat products. Arch Lebensmittelhyg 51:102–104

    CAS  Google Scholar 

  • Harvey RB, Huff WE, Kubena LF, Phillips DT (1989) Evaluation of diets contaminated with aflatoxin and ochratoxin fed to growing pigs. Am J Vet Res 50:1400–1405

    CAS  Google Scholar 

  • Höhler D, Südekum KH, Wolffram S, Frohlich AA, Marquardt RR (1999) Metabolism and excretion of ochratoxin A fed to sheep. J Anim Sci 77:1217–1223

    Google Scholar 

  • Hult K, Teiling A, Gatenbeck S (1976) Degradation of ochratoxin A by a ruminant. Appl Environ Microbiol 32:443–444

    CAS  Google Scholar 

  • International Agency for Cancer Research (1993) Group 2B: possibly carcinogenic to humans. In: Monographs on the evaluation of carcinogenic risks of chemicals to humans, vol 56. International Agency for Cancer Research, Lyon, pp 245–395

  • Jarczyk A, Jedrychovski L, Wroblewska B, Jêdryczko R (1998) The estimation of contamination levels with fungi and ochratoxin A in grains mixed meals for swine in Nort-East Poland. Pol J Food Nutr Sci 7/48(4):631–644

    Google Scholar 

  • Jarczyk A, Bancewicz E, Jêdryczko R (2008) An attempt at inactivitation of ochratoxin A in pigs’ feed with two feed added adsorbents. Anim Sci Pap Rep 26:269–276

    CAS  Google Scholar 

  • Kane A, Creppy EE, Roth A, Röschenthaler R, Dirheimer G (1986) Distribution of the [3H]-label from low doses of radioactive ochratoxin A ingested by rats, and evidence for DNA single-strand breaks caused in liver and kidneys. Arch Toxicol 58:219–224

    Article  CAS  Google Scholar 

  • Kumar M, Dwivedi P, Sharma AK, Singh ND, Patil RD (2007) Ochratoxin A and citrinin nephrotoxicity in New Zealand white rabbits: an ultrastructural assessment. Mycopathologica 163:21–30

    Article  CAS  Google Scholar 

  • Lioi MB, Santoro A, Barbieri R, Salzano S, Ursini MV (2004) Ochratoxin A and zearalenone: a comparative study on genotoxic effects and cell death induced in bovine lymphocytes. Mutat Res 557:19–27

    CAS  Google Scholar 

  • Lippold CC, Stothers SC, Frohlich AA, Boila RJ, Marquardt RR (1992) Effects of periodic feeding of diets containing ochratoxin A on the performance and clinical chemistry of pigs from 15 to 50 kg body weight. Canadian J Anim Sci 72:135–146

    Article  CAS  Google Scholar 

  • Malagutti L, Zannotti M, Scampini A, Sciaraffia F (2005) Effects of ochratoxin A on heavy pig production. Anim Res 54:179–184

    Article  CAS  Google Scholar 

  • Marquardt RR, Frohlich AA (1992) A review of recent advances in understanding ochratoxicosis. J Anim Sci 70:3968–3988

    CAS  Google Scholar 

  • Meyer D, Harvey JW (2004) Hepatobiliary and skeletal muscle enzymes and liver function tests. In: Meyer D, Harvey JW (eds) Veterinary Laboratory medicine: interpretation and diagnosis. WB Saunders Co, St. Louis, MO, pp 169–192

    Google Scholar 

  • Mir MS, Dwivedi P (2010) Ochratoxin A-induced serum biochemical alterations in New Zealand white rabbits (Oryctolagus cuniculus). Turk J Vet Anim Sci 34(6):525–531

    CAS  Google Scholar 

  • Pepeljnjak S, Cvetnić Z, Šegvić Klarić M (2008) Ochratoxin A and zearalenon: cereals and feed contamination in Croatia (1977–2007) and influence on animal and human health. Krmiva 50(3):147–159

    Google Scholar 

  • Pfohl-Leszkowicz A, Manderville RA (2007) Ochratoxin A: an overview on toxicity and carcinogenicity in animals and humans. Mol Nutr Food Res 51:61–99

    Article  CAS  Google Scholar 

  • Pohland AE, Nesheim S, Friedman L (1992) Ochratoxin A: a review. Pure Appl Chem 64(7):1029–1046

    Article  CAS  Google Scholar 

  • Purchase LFH, Theron JJ (1968) The acute toxicity of ochratoxin A to rats. Food Cosmet Toxicol 6:479–483

    Article  CAS  Google Scholar 

  • Raina JS, Roy KS, Singh B (1991) Biochemical and histochemical studies in experimental mycotoxicosis in chicks. Indian J Anim Sci 62:1276–1281

    Google Scholar 

  • Raja AV, Saikumur G, Sharma R (2008) Ochratoxicosis in swine: clinical and pathological changes following prolonged exposure to ochratoxin A. Indian J Anim Sci 78:922–928

    Google Scholar 

  • Ramaiah SK (2007) A toxicologist guide to the diagnostic interpretation of hepatic biochemical parameters. Food Chem Toxicol 45:1551–1557

    Article  CAS  Google Scholar 

  • Schaefer JL, Hamilton PB (1986) Occurrence and clinical manifestations of ochratoxicosis. In: Richard JL, Thurston JR (eds) Diagnosis of mycotoxicoses. Martinus Nijhoff Publishers, Dordrecht, pp 43–50

    Chapter  Google Scholar 

  • Stoev S, Kunev I, Radic B (1997) Haematological, biochemical and toxicological investigations in spontaneous cases of mycotoxic nephropathy (ochratoxicosis) in pigs. Bulgarian J Agri Sci 3:507–516

    Google Scholar 

  • Stoev SD, Vitanov S, Anguelov G, Petkova-Bocharova T, Creppy EE (2001) Experimental mycotoxic nephropathy in pigs provoked by a diet containing ochratoxin A and penicillic acid. Vet Res Comm 25:205–223

    Article  CAS  Google Scholar 

  • Stoev SD, Gundasheva D, Zarkov I, Mircheva I, Zapryanova D, Denev S, Mitev Y, Daskalov H, Dutton M, Mwanza M, Schneider YJ (2011) Experimental mycotoxic nephropathy in pigs provoked by a mouldy diet containing ochratoxin A and fumonisin B1. Exp Toxicol Pathol. doi:10.1016/j.etp.2011.01.008

    Google Scholar 

  • Stormer FC, Lea T (1995) Effects of ochratoxin A upon early and late events in human T-cell proliferation. Toxicology 95:45–50

    Article  CAS  Google Scholar 

  • Strayer L (1981) Biochemistry, 2nd edn. W. H. Freeman and Company, San Francisco, CA

    Google Scholar 

  • Walker R, Larsen JC (2005) Ochratoxin A: previous risk assessments and issues arising. Food Addit Contam 22(Suppl 1):6–9

    Article  CAS  Google Scholar 

  • Winnicka A (2003) Wartości referencyjne podstawowych badań laboratoryjnych w weterynarii (Reference values of basal veterinary laboratory examinations). Warsaw University of Agriculture (SGGW Warszawa) (in Polish)

Download references

Author information

Authors and Affiliations

  1. Department of Veterinary Public Health, Croatian Veterinary Institute, Savska 143, 10000, Zagreb, Croatia

    Jelka Pleadin, Nina Perši, Mario Mitak, Svjetlana Terzić, Ana Vulić & Mate Brstilo

  2. Dubravica Swine Farm, Ltd., Pavla Štoosa 109, 10293, Dubravica, Croatia

    Dinka Milić

Authors
  1. Jelka Pleadin
    View author publications

    Search author on:PubMed Google Scholar

  2. Nina Perši
    View author publications

    Search author on:PubMed Google Scholar

  3. Mario Mitak
    View author publications

    Search author on:PubMed Google Scholar

  4. Svjetlana Terzić
    View author publications

    Search author on:PubMed Google Scholar

  5. Dinka Milić
    View author publications

    Search author on:PubMed Google Scholar

  6. Ana Vulić
    View author publications

    Search author on:PubMed Google Scholar

  7. Mate Brstilo
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Jelka Pleadin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pleadin, J., Perši, N., Mitak, M. et al. Biochemical Changes in Pig Serum After Ochratoxin A Exposure. Bull Environ Contam Toxicol 88, 1043–1047 (2012). https://doi.org/10.1007/s00128-012-0615-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00128-012-0615-x

Keywords

Profiles

  1. Ana Vulić View author profile