Abstract
In the present study effects of herbicides glyphosate (GP), alachlor (AL) and maleic hydrazide (MH) is studied on mitotic cells of Trigonella foenum-graecum L. Seeds of T. foenum-graecum L. treated with a series of concentrations ranging from 0.1%, 0.2%, 0.3%, 0.4% and 0.5% for 1, 2 and 6 h and their effect on mitotic index and chromosomal aberrations was studied. The results indicate that these herbicides reduced mitotic index in dose-dependent manner. In addition, increase in the percentage of abnormal mitotic plates was observed in herbicide treated groups which was both concentration and time dependent. Commonly observed abnormalities were c-mitosis, laggards, bridges, stickiness, c-anaphase, precocious separation, un-equal distribution and fragments. The result of the present investigation indicates that commonly used herbicides GP, AL and MH have significant genotoxic effect on T. foenum-graecum plant.
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Acknowledgments
The authors are grateful to Mr Z. Shah and Prof S. Nasir Ali, Saifia Education Society, Bhopal, India, for providing the necessary laboratory facilities. Thanks are also due to DR.S.S. Khan, Department of Microbiology, Saifia Sciences PG College, Bhopal for his constant help.
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Siddiqui, S., Meghvansi, M.K. & Khan, S.S. Glyphosate, Alachor and Maleic Hydrazide have Genotoxic Effect on Trigonella foenum-graecum L.. Bull Environ Contam Toxicol 88, 659–665 (2012). https://doi.org/10.1007/s00128-012-0570-6
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DOI: https://doi.org/10.1007/s00128-012-0570-6