The study has demonstrated that anticancer drug 5-fluorouracil causes acute toxicity and interferes with the growth of green microalgae, Scenedesmus vacuolatus. It accumulates in microalgae biomass with bioaccumulation factor of 1.84 × 104 and further integrates into the DNA and RNA of microalgae. In addition, the labelled microalgae genome is transferred into protozoan Tetrahymena pyriformis on feeding and is retained in the food vacuoles of predator organisms. This biotransfer of labelled 5-fluorouracil via genomic material was evaluated using radioactivity in Tetrahymena cell pellets though radioactivity did not detect anticancer drug in the genome of the predator organism.
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This research was supported and funded by Helmholtz Zentrum München-German Research Center for Environmental Health, Ingolstädter Landstrasse 1, D-85764 Neuherberg, Germany. The authors want to thank the Institute of Plant Physiology, University of Göttingen, Germany, and Institute of Biology: Ecotoxicology and Biochemistry, Free University of Berlin, Germany, for kindly providing microalgae and protozoan culture.
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Ud-Daula, A., Pfister, G. & Schramm, KW. 5-Fluorouracil Accumulation in Green Microalgae and its Biogenetic Transfer into Ciliate Protozoan. Bull Environ Contam Toxicol 88, 548–554 (2012). https://doi.org/10.1007/s00128-012-0536-8
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