Abstract
To pursue an effective way to control freshwater algae, four extracts from a submerged macrophyte Hydrilla verticillata (Linn.f.) Royle were tested to study its inhibitory effects on Anabaena flos-aquae FACHB-245 and Chlorella pyrenoidosa Chick FACHB-9. Extract with the highest inhibiting ability was further studied in order to reveal the inhibitory mechanism. The results demonstrated that H. verticillata extracts inhibited the growth of A. flos-aquae and C. pyrenoidosa, and methanol extract had the highest inhibiting ability. The mechanism underlying the algal growth inhibition involves the superoxide anion radical generation that induces the damage of cell wall and release of intracellular components.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
American public health association (1985) The assay of phytoplankton chlorophyll the normal checking methods of water and waste water. The Chinese Built Industry Publish, Beijing, pp 901–904
Chen C-Z, Cooper SL (2002) Interactions between dendrimer biocides and bacterial membranes. Biomaterials 23:3359–3368
Denyer SP (1995) Mechanisms of action of antibacterial biocides. Int Biodeterior Biodegrad 36:227–245
Denyer SP, Stewart GS (1998) Mechanisms of action of disinfectants. Int Biodeterior Biodegrad 41:261–268
He C-Q, Wang C-K (2001) The allelopathic effect of Acorus tatarinowii upon algae. J Environ Sci 13(4):481–484
Jeppesen E, Sondergaard M, Christoffersen K (1998) The structuring role of submerged macrophytes in lakes. Ecological Studies. Springer. New York 131:423
Jin Z-H, Zhuang Y-Y, Dai S-G (2003) Isolation and identification of extracts of Eichhornia crassipes and their allelopathic effects on algae. Bull Environ Contam Toxicol 71:1048–1052
Körner S, Nicklisch A (2002) Allelopathic growth inhibition of selected phytoplankton species by submerged macrophytes. J Phycol 38(5):862–871
Li S-H, Zhu H, Xia Y-Z, Yu M-J, Lin K-S, Ye Z-Y, Chen Y-X (1959) The mass culture of unicellular green algae. Acta Hydrobiol Sinica 4:462–472
Nakai S, Inoue Y, Hosomi M (2001) Algal growth inhibition effects and inducement modes by plant-producing phenols. Water Res 35(7):1855–1859
Nakai S, Yamada S, Hosomi M (2005) Anti-cyanobacterial fatty acids released from Myriophyllum spicatum. Hydrobiologia 543:71–78
Ozimek T, Gulati RD, Van Donk E (1990) Can macrophytes be useful in biomanipulation of lakes? The lake Zwemlust example. Hydrobiologia 200/201:399–407
Wang L-X, Wu G-R, Wang J-N (2004) The inhibition of Hydrilla verticillata (Linn.f.) Royle on Microcystis aeruginosa. J Lake Sci 16(4):337–341
Xi Y-L, Dong L-L, Ge Y-L, Liu G-Y (2004) Resting egg production of Brachionus calyciflorus (Rotifera) at different water temperatures. J Freshw Ecol 19(4):567–574
Xiao H-S, He W-J, Fu W-Q (1999) A spectrophotometer method testing oxygen radicals. Prog Biochem Biophys 26(2):180–182
Zhang T-T, Chen C-P, He M, Wu A-P, Nie L-W (2007a) Allelopathic effects of several higher aquatic plants on algae. J Biol 24(4):16–20
Zhang T-T, Wu A-P, He M, Chen C-P, Nie L-W (2007b) The allelopathic inhibition and allelopathic mechanism of phenolic acids on algae in water bloom. China Environ Sci 27(4):472–476
Acknowledgments
This study was supported by the National Natural Science Foundation of China (No.30870429) and The Provincial Laboratory for Conservation and Utilization of Important Biological Resources in Anhui. The authors would like to thank Professor Yi-Long Xi and Shou-Biao Zhou for their generous help during the experiment.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zhang, TT., He, M., Wu, AP. et al. Inhibitory Effects and Mechanisms of Hydrilla verticillata (Linn.f.) Royle Extracts on Freshwater Algae. Bull Environ Contam Toxicol 88, 477–481 (2012). https://doi.org/10.1007/s00128-011-0500-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00128-011-0500-z