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Characterization of Chromophoric Dissolved Organic Matter (CDOM) in Rainwater Using Fluorescence Spectrophotometry


The fluorescence excitation–emission matrix of Chromophoric dissolved organic matter (CDOM) samples from rainwater collected at Rameswaram, Tamilnadu, India are analysed. Total five peaks were observed for humic/marine and protein likes substances respectively. The peak A and C intensities varies form 1.98 ± 0.28 and 0.97 ± 0.11 QSU respectively represents humic like substances. The peak B and T intensities varies from 3.94 ± 0.75 and 7.42 ± 1.43 QSU showed association of protein like substances whereas peak M intensities varies from 1.92 ± 0.37 QSU indicates marine contribution. Among the fluorophores, the following sequence were observed as T > B > A > M > C which indicates dominance of Tryptophan like substances in rainwater. The average peak T/C ratios was observed as 7.88 ± 2.2 indicates microbial contamination by Tryptophan-like substances with the high biological activity and low volatility.

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The authors thank the Director, National Environmental Engineering Research Institute (NEERI), Nehru Marg, Nagpur for continuous encouragement and keen interest in publishing this work. Authors also acknowledged Prof. Weidong Guo, State Key Laboratory of Marine Sciences, Xiamen University, Xiamen, China for their valuable guidance on fluorescence spectroscopic studies.

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Correspondence to P. R. Salve.

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Salve, P.R., Lohkare, H., Gobre, T. et al. Characterization of Chromophoric Dissolved Organic Matter (CDOM) in Rainwater Using Fluorescence Spectrophotometry. Bull Environ Contam Toxicol 88, 215–218 (2012).

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  • CDOM
  • Fluorescence intensities
  • HIX
  • BIX