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Chronic Toxicity of Erythromycin Thiocyanate to Daphnia magna in a Flow-Through, Continuous Exposure Test System


Approval of a new animal drug application for AQUAMYCIN 100® (erythromycin thiocyanate; ET) to treat freshwater salmonid species with bacterial kidney disease is being pursued in the US. As part of the approval process, ET’s impact on an aquatic environment had to be described in an environmental assessment. The environmental assessment was lacking data to characterize the effect ET would have on a chronically exposed aquatic invertebrate organism. A major step to fulfilling the environmental assessment was completed after conducting a comprehensive study continuously exposing Daphnia magna to ET for 21 days. Results indicated that the no observable effect concentration for ET was 179 μg/L.

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  1. American Standards for Testing and Materials Designation E 1193-97 (1997) Standard guide for conducting Daphnia magna life-cycle toxicity tests. ASTM International, West Conshokocken

    Google Scholar 

  2. Dojmi di Delupis G, Macri A, Civitareale C, Migliore L (1992) Antibiotics of zootechnical use: effects of actue high and low dose contamination on Daphnia magna Straus. Aquat Toxicol 22:53–60

    Article  CAS  Google Scholar 

  3. Haney JF, Hall DJ (1973) Sugar-coated Daphnia: a preservation technique for Cladocera. Limnol Oceanogr 18:331–333

    Article  Google Scholar 

  4. Isidori M, Lavorgna M, Nardelli A, Pascarella L, Parrella A (2005) Toxic and genotoxic evaluation of six antibiotics on non-target organisms. Sci Total Environ 346:87–98

    Article  CAS  Google Scholar 

  5. Kaplan EL, Meier P (1958) Nonparametric estimation from incomplete observations. J American Statist Assn 53:457–481

    Article  Google Scholar 

  6. McCullagh P, Nelder JA (1989) Generalized linear models, 2nd edn. Chapman and Hall, England

    Google Scholar 

  7. Meinertz JR, Greseth SL, Gaikowski MP, Schmidt LJ (2008) Chronic toxicity of hydrogen peroxide to Daphnia magna in a continuous exposure, flow-through test system. Sci Total Environ 392:225–232

    Article  CAS  Google Scholar 

  8. Meinertz JR, Schreier TM, Bernardy JA, Franz JL (2010) Chronic toxicity of diphenhydramine hydrochloride and erythromycin thiocyanate to daphnia, Daphnia magna, in a continuous exposure test system. Bull Environ Contam Toxicol 85:447–451

    Article  CAS  Google Scholar 

  9. SAS Institute Incorporated (2004) Version 9.1 edition. Cary, North Carolina

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We thank Mark Gaikowski of the USGS Upper Midwest Environmental Sciences Center for statistical support.

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Correspondence to Jeffery R. Meinertz.

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Meinertz, J.R., Schreier, T.M. & Bernardy, J.A. Chronic Toxicity of Erythromycin Thiocyanate to Daphnia magna in a Flow-Through, Continuous Exposure Test System. Bull Environ Contam Toxicol 87, 621–625 (2011).

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  • Toxicity of erythromycin thiocyanate to Daphnia
  • Assessing the environmental impact of an aquaculture drug