Sublethal Effects of Waterborne Herbicides in Tropical Freshwater Fish

  • Stéfani Cibele Rossi
  • Manuela Dreyer da Silva
  • Laercio Dante Stein Piancini
  • Ciro Alberto Oliveira Ribeiro
  • Marta Margarete Cestari
  • Helena Cristina Silva de AssisEmail author


The study evaluated the sublethal effects of the herbicides glyphosate (Roundup) and diuron (Hexaron) and the mixture of them, used extremely in agriculture, through biomarkers in fish. The glutathione S-transferase activity increased (74%) and catalase activity decreased (37%) at the higher exposure concentration of Hexaron in comparison to the control group, suggesting an activation of this metabolism route. Membrane damage was observed at the higher exposure of Roundup and in the mixture group compared to the control group, which can be related to the nuclear alterations observed in these exposed groups. The cholinesterase activity was also inhibited (37%) in mixture group compared to the control group and no gill morphology damage was found. The results suggested a potential synergic effect in some analysed parameters.


Astyanax sp. Bioassay Biomarkers Freshwater fish Herbicides 



This research was supported in part by CNPq (Brazilian Agency for Science and Technology).


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Stéfani Cibele Rossi
    • 1
  • Manuela Dreyer da Silva
    • 1
  • Laercio Dante Stein Piancini
    • 2
  • Ciro Alberto Oliveira Ribeiro
    • 3
  • Marta Margarete Cestari
    • 2
  • Helena Cristina Silva de Assis
    • 1
    Email author
  1. 1.Department of PharmacologyFederal University of ParanáCuritibaBrazil
  2. 2.Department of GeneticFederal University of ParanáCuritibaBrazil
  3. 3.Department of Cellular BiologyFederal University of ParanáCuritibaBrazil

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