Toxicological Responses of Cyprinus carpio Exposed to a Commercial Formulation Containing Glyphosate
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The effects of commercial glyphosate herbicide formulation on the activity of acetylcholinesterase (AChE) enzyme and oxidative stress were studied in Cyprinus carpio exposed for 96 h to 0.0, 0.5, 2.5, 5.0 and 10.0 mg/L and then allowed to equal recovery period in water without herbicide. The activity of AChE was inhibited in the brain and in the muscle after exposure. However, after recovery period brain and muscle AChE activity increased. Brain thiobarbituric acid reactive species (TBARS) were measured as an indicator of oxidative stress. Increased TBARS levels were observed with all concentrations tested of the glyphosate formulation, and remained increased after the recovery period. The results recorded clearly indicate lipid peroxidation and anti-AChE action induced by Roundup® exposure.
KeywordsGlyphosate Carp AChE TBARS
We would like to thanks the Universidade Federal de Santa Maria (UFSM) for the support and the facilities, to CAPES for the student Assistantship and the undergraduate student salary and CNPq by research fellowship to Vania Lucia Loro.
- Cox C (1998) Glyphosate (Roundup). J Pestic Reform 18:3–17Google Scholar
- Glusczak L, Miron DS, Moraes BS, Simões RR, Schetinger MRC, Morsch VM, Loro VL (2007) Acute effects of glyphosate herbicide on metabolic and enzymatic parameters of silver catfish (Rhamdia quelen). Comp Biochem Physiol C 146:519–524Google Scholar
- Langiano VC, Martinez CBR (2008) Toxicity and effects of a glyphosate-based herbicide on the neotropical fish Prochilodus lineatus. Comp Biochem Phys C 147:222–231Google Scholar
- Miron D, Crestani M, Schetinger MR, Morsch VM, Baldisserotto B, Tierno MA, Moraes G, Vieira VL (2005) Effects of the herbicides clomazone, quinclorac and metsulfuron methyl on acetylcholinesterase activity in the silver catfish (Rhamdia quelen) (Heptapteridae). Ecotox Environ Safe 61:398–403CrossRefGoogle Scholar
- Rodrigues BN, Almeida FS (2005) Guia de Herbicidas, 5th edn. UEL editora, Londrina, p 592Google Scholar
- WHO (1994) Glyphosate. Enviroment health criteria no 159. World Health Organization, GenevaGoogle Scholar