Abstract
Green-lipped mussels (Perna viridis) were exposed to waterborne tributyltin chloride (TBTCl) at different concentrations (0.2, 0.4 and 0.8 μg L−1) for up to 72 h. Accumulated TBTCl in Perna viridis correlated linearly with the exposure concentrations of 0.2 μg L−1(R2 = 0.772), 0.4 μg L−1(R2 = 0.952), and 0.8 μg L−1(R2 = 0.909). The results of superoxide dismutase (SOD), glutathione peroxidase (GPx) and malondialdehyde (MDA) all decreased in gill tissues after 24 h of exposure, but the hepatic SOD and the hepatic GPx showed either little or no effect on exposure of TBTCl solutions. Analysis using the Spearman rank correlation coefficient showed the hepatic GPx activity appeared to have a significant negative correlativity (Rs = −0.42) with the exposed TBTCl concentrations, and the hepatic MDA was significantly negatively correlated (Rs = −0.33) with the tissue TBTCl concentrations. Conversely, a significant positive correlation (Rs = 0.60) was shown between the gill MDA contents and exposure time. This study illustrates oxyradical scavenger GPx best correlated with stress level of pollutants among the various antioxidant parameters.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Antizar-Ladislao B (2008) Environmental levels, toxicity and human exposure to tributyltin (TBT)-contaminated marine environment: a review. Environ Int 34:292–308
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Burton ED, Phillips IR, Hawker DW (2004) Sorption and desorption behavior of tributyltin with natural sediments. Environ Sci Technol 38:6694–6700
Cheung CCC, Zheng GJ, Li AMY, Richardson BJ, Lam PKS (2001) Relationships between tissue concentrations of polycyclic aromatic hydrocarbons and antioxidative responses of marine mussels, Perna viridis. Aquat Toxicol 52:189–203
Emmanouil C, Green RM, Willey FR, Chipman JK (2008) Oxidative damage in gill of Mytilus edulis from Merseyside, UK, and reversibility after depuration. Environ Pollut 151:663–668
Harino H, Yamamoto Y, Eguchi S, Kawai SC, Kurokawa Y, Arai T, Ohji M, Okamura H, Miyazaki N (2007) Concentrations of antifouling biocides in sediment and mussel samples collected from Otsuchi Bay, Japan. Arch Environ Con Tox 52:179–188
Huang ZY, Chen YX, Zhao Y, Zuo ZH, Chen M, Wang CG (2005) Antioxidant responses in Meretrix meretrix exposed to environmentally relevant doses of tributyltin. Environ Toxicol Pharmacol 20:107–111
Inoue S, Oshimaa YJ, Usukic H, Hamaguchic M, Hanamurac Y, Kaid N, Shimasakia Y, Honjoa T (2006) Effects of tributyltin maternal and/or waterborne exposure on the embryonic development of the Manila clam, Ruditapes philippinarum. Chemosphere 63:881–888
Jiang GB, Zhou QF, Liu JY, Wu DJ (2001) Occurrence of butyltin compounds in the waters of selected lakes, rivers and coastal environments from China. Environ Pollut 115:81–87
Kannan K, Tanabe S, Iwata H, Tatsukawa R (1995) Butyltins in muscle and liver of fish collected from certain Asian and Oceanian countries. Environ Pollut 90:279–290
Lagadic L, Coutellec MA, Caquet T (2007) Endocrine disruption in aquatic pulmonate molluscs: few evidences, many challenges. Ecotoxicology 16:45–59
Landmeyer JE, Tanner TL, Watt BE (2004) Biotransformation of tributyltin to tin in freshwater river-bed sediments contaminated by an organotin release. Environ Sci Technol 38:4106–4112
Laughlin RB Jr, French W (1988) Concentration dependence of bis(tributyl)tin oxide accumulation in the mussel, Mytilus edulis. Environ Toxicol Chem 7:1021–1026
Lee CC, Hsieh CY, Tien CJ (2006) Factors influencing organotin distribution in different marine environmental compartments, and their potential health risk. Chemosphere 65:547–559
Meador JP, Rice CA (2001) Impaired growth in the polychaete Armandia brevis exposed to tributyltin in sediment. Mar Environ Res 51:113–129
Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358
Oyanagui Y (1984) Reevaluation of assay methods and establishment of kit for superoxide dismutase activity. Anal Biochem 142:290–296
Porte C, Biosca X, Solé M, Albaigés J (2001) The integrated use of chemical analysis, cytochrome P450 and stress proteins in mussels to assess pollution along the Galician coast (NW Spain). Environ Pollut 112:261–268
Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG (1973) Selenium: biochemical role as a component of glutathione peroxidase. Science 179:588–590
Tsang CK, Lau PS, Tam N, Wong YS (1999) Biodegradation capacity of tributyltin by two Chlorella species. Environ Pollut 105:289–297
Vanssay Ed, McDonald GD, Khare BN, Gomez-Ariza JL, Morales E, Giraldez I (1999) Uptake and elimination of tributyltin in clams, Venerupis decussata. Mar Environ Res 47:399–413
Wu YQ, Wang CG, Wang Y, Zhao Y, Chen YX, Zuo ZH (2007) Antioxidant responses to benzo [a] pyrene, tributyltin and their mixture in the spleen of Sebasticus marmoratus. J Environ Sci China 19:1129–1135
Acknowledgments
This work was supported by grants from the Research Project of Public Welfare, Ministry of Science and Technology of China (No. 2005DIB3J021), the Science and technology projects of Guangdong Province, China (No. 2009B030600001) and Central Public-interest Scientific Institution Basal Research Fund of China (No. 2009YD01).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Chen, HG., Jia, XP., Cai, WG. et al. Antioxidant Responses and Bioaccumulation in Green-lipped Mussels (Perna Viridis) Under Acute Tributyltin Chloride Exposure. Bull Environ Contam Toxicol 87, 506–511 (2011). https://doi.org/10.1007/s00128-011-0390-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00128-011-0390-0