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Ecotoxicological Assessment of Cobalt Used as Supplement in the Diet of Common Carp Cyprinus carpio


Experiments were performed in the laboratory to determine if excess levels of Co used as dietary supplement (0.0, 0.05, 0.10 and 1.0%) to enhance growth of the fish Cyprinus carpio was safe for aquatic organisms. Lethal concentrations of Co for tadpole of toad Bufo melanostictus (96 h LC50, 17.2 mg/L), oligochaet worm Branchiura sowerbyi (96 h LC50, 179 mg/L) and crustacean zooplankton Diaptomus forbesi (96 h LC50, 1.5 mg/L) were compared with the concentration of Co in the medium leached from the unused diets and faeces. The results indicated that the Co leached from diet containing 1.0% Co was ecotoxicologically unsafe for crustacean zooplankton.

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  1. Anadu DI, Anozie OC, Anthony AD (1990) Growth responses of Tilapia zillii fed diets containing various levels of ascorbic acid and cobalt chloride. Aquaculture 88:329–336

    Article  CAS  Google Scholar 

  2. APHA (American Public Health Association) (1995) Standard methods for the examination of water and wastewater. APHA, Washington

    Google Scholar 

  3. Biesinger KE, Christensen GM (1972) Effects of various metals on survival, growth, reproduction, and metabolism of Daphnia magna. J Fish Res Board Can 29:1691–1700

    Article  CAS  Google Scholar 

  4. Churnoff B (1975) A method for wet digestion of mullet tissue for heavy metal analysis. Trans Am Fish Soc 104:803–804

    Article  Google Scholar 

  5. Das BK, Kaviraj A (1994) Individual and interactive lethal toxicity of cadmium, potassium permanganate and cobalt chloride to fish, worm and plankton. Geobios 21(4):223–227

    CAS  Google Scholar 

  6. Ewell WS, Gorsuch JW, Kringle RO, Robillard KA, Spiegel RC (1986) Simultaneous evaluation of the acute effects of chemicals on seven aquatic species. Environ Toxicol Chem 5(9):831–840

    Article  CAS  Google Scholar 

  7. Gomez KA, Gomez AA (1984) Statistical procedures for agricultural research, 2nd edn. Wiley, New York

    Google Scholar 

  8. Hasan MR (2001) Nutrition and feeding for sustainable aquaculture development in the third millennium. In: Subasinghe RP, Bueno P, Phillips MJ, Hough C, McGladdery SE, Arthur JR (eds) Aquaculture in the third millennium, technical proceedings of the conference on aquaculture in the third millennium, Bangkok, Thailand, 20–25 February 2000. NACA, Bangkok, pp 193–219

    Google Scholar 

  9. Khangarot BS (1991) Toxicity of metals to a freshwater tubificid worm, Tubifex tubifex (Muller). Bull Environ Contam Toxicol 46:906–912

    Article  CAS  Google Scholar 

  10. Khangarot BS, Ray PK (1989) Investigation of correlation between physicochemical properties of metals and their toxicity to the water flea Daphnia magna Straus. Ecotoxicol Environ Safe 18(2):109–120

    Article  CAS  Google Scholar 

  11. Khangarot BS, Sehgal A, Bhasin MK (1985) “Man and biosphere” Studies on the Sikkim Himalayas. Part 5: Acute toxicity of selected heavy metals on the tadpoles of Rana hexadactyla. Acta Hydroch Hydrob 13(2):259–263

    Article  CAS  Google Scholar 

  12. Mahmoud SA (2009) Effect of different artificial diets on growth rate condition and histological structure of Nile tilapia (Oreochromis niloticus). Res J Fish Hydrobiol 4(1):29–34

    Google Scholar 

  13. Mukherjee S, Kaviraj A (2009) Evaluation of growth and bioaccumulation of cobalt in different tissues of common carp Cyprinus carpio fed cobalt supplemented diets. Acta Ichthyol Piscat 39(2):87–93

    Article  Google Scholar 

  14. Nagpal NK (2004) Technical report - water quality guidelines for cobalt. Ministry of Water, Land, and Air Protection, BC. Available at

  15. Páez-Osuna F, Tron-Mayen L (1995) Distribution of heavy metals in tissues of the shrimp Penaeus californensis from the Northwest coast of Mexico. Bull Environ Contam Toxicol 55:209–215

    Article  Google Scholar 

  16. Vanloon JC (1980) Analytical atomic absorption spectroscopy. Academic, New York

    Google Scholar 

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The authors are thankful to University of Kalyani for providing necessary laboratory facilities and financial assistance for this research.

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Correspondence to Anilava Kaviraj.

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Mukherjee, S., Kaviraj, A. Ecotoxicological Assessment of Cobalt Used as Supplement in the Diet of Common Carp Cyprinus carpio . Bull Environ Contam Toxicol 87, 527 (2011).

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  • Cobalt
  • Diet
  • Leaching
  • LC50