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High Performance Liquid Chromatography Method for Residues Analysis of Thidiazuron in Apple and Soil

Abstract

A rapid, sensitive and reliable analytical method for thidiazuron residues in apple and soil was established. The residual levels of the pesticide in apple and soil were determined by high performance liquid chromatography (HPLC) with UV detector. Samples of apple and soil were extracted with acetonitrile–water solutions, and then cleaned up by Florisil or C18 cartridges. The results showed good linearity (r 2 = 1.000) over the concentration range of 0.01–5.0 mg/L. Limits of quantification (LOQ) of the method were 0.01 mg/kg for both soil and apple. Recovery from the apple and soil samples were 83.36%–84.08% and 85.27%–89.83%, respectively, and the corresponding relative standard deviations (RSDs) of the recovery data were 0.155%–0.524% and 0.475%–4.79% for the three fortified levels (0.01, 0.1, 0.5 mg/kg). The analyte in the samples were further confirmed by electrospray ionization tandem mass spectrometry (ESI–MS/MS). It was demonstrated that the proposed method was simple and efficient, and particularly suitable for detecting thidiazuron residues in apple and soil.

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Acknowledgments

This work was supported by grants from Jiangsu Huifeng Agrochemical Co., Ltd. (Jiangsu, China) and the Institute for the Control of Agrochemicals, Ministry of Agriculture of the People’s Republic of China.

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Correspondence to Ji-Ye Hu.

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Hu, JY., Hu, YQ., Chen, Y. et al. High Performance Liquid Chromatography Method for Residues Analysis of Thidiazuron in Apple and Soil. Bull Environ Contam Toxicol 87, 448 (2011). https://doi.org/10.1007/s00128-011-0383-z

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Keywords

  • Thidiazuron
  • Residues determination
  • Solid-phase extraction
  • High performance liquid chromatography with UV detector