Skip to main content

Changes in Microbial Populations and Enzyme Activities During Nitrogen Biodegradation of Domestic Sewage Treatment in the Subsurface Wastewater Infiltration System (SWIS)

Abstract

During the process of domestic sewage treatment in the Subsurface Wastewater Infiltration System (SWIS), changes in the microbial populations (nitrifying and denitrifying bacteria) and enzyme activities (urease, nitrate reductase and nitrite reductase) involved in the nitrogen removal process were evaluated over a 2-year period. The results showed nitrifying bacteria number declined with depths increasing, while denitrifying bacteria increased, both of which increased nearer the inlet. The depth for nitrate reductase activity from high to low in sequence was 0.3, 0.5, 0.7, 0.9 and 1.1 m. For nitrite reductase, the sequence was 0.5, 0.3, 0.7, 0.9 and 1.1 m. Urease and nitrite reductase activities were in positive correlation with the total nitrogen removal efficiency, with correlation coefficients 0.8662 and 0.9140, respectively and could be alternative to monitor the nitrogen biodegradation process in SWIS.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3

References

  1. Agency ChineseEnvironmentalProtection (2002) Methods for water and wastewater analysis. Environmental Science Publishing House of China, Beijing (in Chinese)

    Google Scholar 

  2. Arienzo M, Christen EW, Quayle W, Kumar A (2009) A review of the fate of potassium in the soil-plant system after land application of wastewaters. J Hazard Mater 164:415–422

    Article  CAS  Google Scholar 

  3. Babatunde AO, Zhao YQ, O’Neill M, O’Sullivan B (2008) Constructed wetlands for environmental pollution control: a review of developments, research and practice in Ireland. Environ Int 34:116–126

    Article  CAS  Google Scholar 

  4. Belinda EH, Tim DF, Ana D (2007) Treatment performance of gravel filter medium: implication for design and application of stormwater infiltration systems. Water Res 41:2513–2524

    Article  Google Scholar 

  5. Guan SY (1986) Soil enzyme and the researching methods. Agriculture Publishing House of China, Beijing (in Chinese)

    Google Scholar 

  6. Hati KM, Mandal KG, Misra AK, Ghosh PK, Bandyopadhyay KK (2006) Effect of inorganic fertilizer and farmyard manure on soil physical properties, root distribution, and water-use efficiency of soybean in Vertisols of central India. Bioresour Technol 97:2182–2188

    Article  CAS  Google Scholar 

  7. Head MA, Oleszkiewicz JA (2004) Bioaugmentation for nitrification at cold temperatures. Water Res 38:523–530

    Article  CAS  Google Scholar 

  8. Hsu MJ, Selvaraj K, Agoramoorthy G (2006) Taiwan’s industrial heavy metal pollution threatens terrestrial biota. Environ Pollut 143:327–334

    Article  CAS  Google Scholar 

  9. Kamitani T, Kaneko N (2007) Species-specific heavy metal accumulation patterns of earthworms on a floodplain in Japan. Ecotoxicol Environ Saf 66:82–91

    Article  CAS  Google Scholar 

  10. Kim JY, Sansalone JJ (2008) Event-based size distributions of particulate matter transported during urban rainfall-runoff events. Water Res 42:2756–2768

    Article  CAS  Google Scholar 

  11. Li YH, Li HB, Sun TH, Wang X (2011) Study on nitrogen removal enhanced by shunt distributing wastewater in a constructed subsurface infiltration system under intermittent operation mode. J Hazard Mater 189:336–341

    Article  CAS  Google Scholar 

  12. Luanmanee S, Boonsook P, Attanandana T, Saitthiti B, Panichajakul C, Wakatsuki T (2002) Effect of intermittent aeration regulation of a multi-soil-layering system on domestic wastewater treatment in Thailand. Ecol Eng 24:415–428

    Article  Google Scholar 

  13. Molle P, Liénard A, Grasmick A, Iwema A (2006) Effect of reeds and feeding operations on hydraulic behavior of vertical flow constructed wetlands under hydraulic overloads. Water Res 40:606–612

    Article  CAS  Google Scholar 

  14. Parveen S, Nazif W, Ahmad MF, Khan A, Khattak IA (2006) Nutritional status of different orchards irrigated with wastewater in district Peshawar. J Agric Biol Sci 1:42–50

    Google Scholar 

  15. Smiles DE (2006) Sodium and potassium in soils of the Murray-Darling basin: a note. Aust J Soil Res 44:727–730

    Article  CAS  Google Scholar 

  16. Tunçsiper B, Ayaz S, Akça L, Gunes K (2009) Performance of a pilot-scale, three-stage constructed wetland system for domestic wastewater treatment. Environ Technol 30:1187–1194

    Article  Google Scholar 

  17. Walid KL, Al-Qodah Z (2006) Combined advanced oxidation and biological treatment processes for the removal of pesticides from aqueous solutions. J Hazard Mater B137:489–497

    Google Scholar 

  18. Xu W, Que-Hee SS (2007) Permeation of a straight oil metalworking fluid through disposable nitrile, chloroprene, vinyl, and latex gloves. J Hazard Mater B147:923–929

    Article  Google Scholar 

Download references

Acknowledgments

The authors would like to thank the National Key Technology R&D Program (no. 2011BAJ06B02), National Natural Science Foundation of China (no. 50908151 and no. 51008198), the Ministry of Science and Technology of the People’s Republic of China “Major projects on control and rectification of water body pollution, Research on Key Technology and its Demonstration of Liaohe River Estuary Land-source Pollution Control and Water Quality Improve” (no. 2008ZX07208-008-04) and the Program for Excellent Talents of Liaoning Province (LR201028) for the financial support of this research.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Haibo Li.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Li, Y., Li, H., Wang, X. et al. Changes in Microbial Populations and Enzyme Activities During Nitrogen Biodegradation of Domestic Sewage Treatment in the Subsurface Wastewater Infiltration System (SWIS). Bull Environ Contam Toxicol 87, 431 (2011). https://doi.org/10.1007/s00128-011-0359-z

Download citation

Keywords

  • Soil treatment
  • Subsurface wastewater infiltration system
  • Nitrogen removal
  • Microbial population
  • Enzyme activity