During the process of domestic sewage treatment in the Subsurface Wastewater Infiltration System (SWIS), changes in the microbial populations (nitrifying and denitrifying bacteria) and enzyme activities (urease, nitrate reductase and nitrite reductase) involved in the nitrogen removal process were evaluated over a 2-year period. The results showed nitrifying bacteria number declined with depths increasing, while denitrifying bacteria increased, both of which increased nearer the inlet. The depth for nitrate reductase activity from high to low in sequence was 0.3, 0.5, 0.7, 0.9 and 1.1 m. For nitrite reductase, the sequence was 0.5, 0.3, 0.7, 0.9 and 1.1 m. Urease and nitrite reductase activities were in positive correlation with the total nitrogen removal efficiency, with correlation coefficients 0.8662 and 0.9140, respectively and could be alternative to monitor the nitrogen biodegradation process in SWIS.
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The authors would like to thank the National Key Technology R&D Program (no. 2011BAJ06B02), National Natural Science Foundation of China (no. 50908151 and no. 51008198), the Ministry of Science and Technology of the People’s Republic of China “Major projects on control and rectification of water body pollution, Research on Key Technology and its Demonstration of Liaohe River Estuary Land-source Pollution Control and Water Quality Improve” (no. 2008ZX07208-008-04) and the Program for Excellent Talents of Liaoning Province (LR201028) for the financial support of this research.
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Li, Y., Li, H., Wang, X. et al. Changes in Microbial Populations and Enzyme Activities During Nitrogen Biodegradation of Domestic Sewage Treatment in the Subsurface Wastewater Infiltration System (SWIS). Bull Environ Contam Toxicol 87, 431 (2011). https://doi.org/10.1007/s00128-011-0359-z
- Soil treatment
- Subsurface wastewater infiltration system
- Nitrogen removal
- Microbial population
- Enzyme activity