Abstract
To assess the toxicity of nonylphenol towards aquatic crustaceans, Neocaridina denticulata were exposed short-term to sublethal concentration (0.001–0.5 mg/L). Following treatment, differentially expressed genes were identified using suppression subtractive hybridization on samples prepared from whole specimens. There were 20 differentially expressed sequence tags that corresponded to known genes and could be divided into six functional classes: defence, translation, metabolism, ribosomal gene expression, respiration, and genes involved in the stress response. Using semi-quantitative RT–PCR, we found that 14 of the differentially expressed sequence tags significantly responded to nonylphenol, including six at a nominal concentration of 0.01 mg/L; among them, 12 genes were down-regulated. These results suggest that under non-lethal concentrations of nonylphenol, the polluted aquatic environment may still present a potential risk to N. denticulata.
Similar content being viewed by others
References
Ahel M, McEvoy J, Giger W (1993) Bioaccumulation of lipophilic metabolites of nonionic surfactants in freshwater organisms. Environ Pollut 709:243–248
Ahel M, Schaffner C, Giger W (1996) Behaviour of alkylphenol polyethoxylate surfactants in the aquatic environment–III occurrence and elimination of their persistent metabolites during infiltration of river water to groundwater. Water Res 30:37–46
Bechmann RK (1999) Effect on the endocrine disrupter nonylphenol on the marine copepod Tisbe battagliai. Sci Total Environ 233:33–46
Canesi L, Lorusso LC, Ciacci C, Bett M, Rocchi M, Pojana G, Marcomini A (2007) Immunomodulation of Mytilus hemocytes by individual estrogenic chemicals and environmentally relevant mixtures of estrogens: In vitro and in vivo studies. Aquat Toxicol 81:36–44
Chung JS (2008) A trehalose 6-phosphate synthase gene of the hemocytes of the blue crab, Callinectes sapidus: cloning, the expression, its enzyme activity and relationship to hemolymph trehalose levels. Saline Syst 4:18
Decker H, Rimke T (1998) Tarantula hemocyanin shows phenoloxidase activity. J Biol Chem 273:25889–25892
Ding WH, Tzing SH, Lo JH (1999) Occurrence and concentrations of aromatic surfactants and their degradation products in river waters of Taiwan. Chemosphere 38:2597–2606
Kirkpatrick AJ, Gerhardt A, Dick JTA, McKenna M, Berges JA (2006) Use of the multispecies freshwater biomonitor to assess behavioral changes of Corophium volutator (Pallas, 1766) (Crustacea, Amphipoda) in response to toxicant exposure in sediment. Ecotoxi Environ Safety 64:298–303
Lu KY, Sung HJ, Sung HH (2009) Differentially enhanced gene expression in hemocytes from Macrobrachium rosenbergii challenged in vivo with lipopolysaccharide. J Invertebr Pathol 100:9–15
Ma J, Zhang D, Jiang J, Cui S, Pu H, Jiang S (2010) Molecular characterization and expression analysis of cathepsin L1 cysteine protease from pearl oyster Pinctada fucata. Fish Shellfish Immunol 29:501–507
Moens LN, van der Ven K, Van Remortel P, Del-Favero J, De Coen WM (2007) Gene expression analysis of estrogenic compounds in the liver of common carp (Cyprinus carpio) using a custom cDNA microarray. J Biochem Mol Toxicol 21:299–311
Nice HE, Thorndyle M, Morrit D, Steele S, Crane M (2000) Development of Crassostrea gigas larvae is affected by 4-nonylphenol. Mar Pollut Bull 40:491–496
Sakurai M, Furuki T, Akao A, Tanaka D, Nakahara Y, Kikawada T, Watanabe M, Okuda T (2008) Vitrification is essential for anhydrobiosis in an African chironomid, Polypedilum vanderplanki. Proc Natl Acad Sci USA 105:5093–5098
Soares A, Guieysse B, Jefferson B, Cartmell E, Lester JN (2008) Nonylphenol in the environment: A critical review on occurrence, fate, toxicity and treatment in wastewaters. Environ Int 34:1033–1049
Staples CA, Dorn PB, Klecka GM, O’Block ST, Harris LR (1998) A review of the environmental fate, effects, and exposures of bisphenol A. Chemosphere 10:2149–2173
Sung HH, Su YJ (2005) Effects of nonylphenol and nonylphenol monoethoxylate on hemocytes of the giant freshwater prawn (Macrobrachium rosenbergii): An in vitro study on viability and immune response. J Fish Soc Taiwan 32:101–111
Sung HH, Ye YZ (2009) Effect of nonylphenol on giant freshwater prawn (Macrobrachium rosenbergii) via oral treatment: toxicity and gene expression of hemocytes. Aquat Toxicol 91:270–277
Victor B, Narayanan M, JonesNelson D (1990) Gill pathology and hemocyte response in mercury exposed Macrobrachium idae (Heller). J Environ Biol 11:61–65
Acknowledgments
This research was supported by the National Science Council, R.O.C (NSC96-2745-B-031-003-URD).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Liu, CL., Sung, HH. Genes are Differentially Expressed at Transcriptional Level of Neocaridina denticulata Following Short-Term Exposure to Nonylphenol. Bull Environ Contam Toxicol 87, 220–225 (2011). https://doi.org/10.1007/s00128-011-0338-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00128-011-0338-4