Skip to main content
SpringerLink
Log in

Parameter Validation of Analytical Methods of Insecticide Residue Analyses in Foods of Animal Origin, Feed and Water

  • Published:
Bulletin of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

The study was conducted to examine the interrelationship and coherence of analytical parameters in method validation. Recovery, sensitivity, linearity, precision and limits of detection (LOD) were tested in six methods for organochlorine and organophosphate insecticides. Compounds that fell out of the stipulated recovery, 70–120%, in a matrix have concurrently failed to meet the requirements for sensitivity (≥0.7), linearity (R2 > 0.99) and precision (<0.2) in the same matrix. Highest LOD was recorded in those compounds and matrices. Different from the conventional point estimate, a new approach was introduced for setting upper and lower confidence limits of the LOD in quantitative analyses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • AOAC/FAO/IAEA/IUPAC (1999) Guidelines for single-laboratory validation of analytical methods for trace-level concentrations of organic chemicals. Available via http://www.iaea.org/trc/pest-qa. Accessed 15 March 2009

  • Barwick VJ, Ellison LR (1999) Measurement uncertainty: approaches to the evaluation of uncertainties associated with recovery. Analyst 124:981–990

    Article  CAS  Google Scholar 

  • CAC (1999) Recommended methods of sampling for the determination of pesticide residues for compliance with MRL. Document No. CAC/GL 33

  • Caldas SS, Demoliner A, Primel EG (2009) Validation of a method using solid phase extraction and liquid chromatography for the determination of pesticide residues in groundwaters. J Brazilian Chem Soc 20:125–132

    Article  CAS  Google Scholar 

  • DFG (Deutsche Forschungsgemeinschaft) (1992) Manual of pesticide residue analysis, vol 2. VCH mbH, Weinheim

  • Hill AR, Reynolds SL (1999) Guidelines for in-house validation of analytical methods for pesticide residues in food and animal feed. Analyst 124:953–958

    Article  CAS  Google Scholar 

  • IUPAC (1995) Nomenclature in evaluation of analytical methods including detection and quantification capabilities. Pure Appl Chem 67:1699–1723

    Article  Google Scholar 

  • Kan CA, Meijer GA (2007) The risk of contamination of food with toxic substances present in animal feed. Anim Feed Sci Technol 133:84–108

    Article  CAS  Google Scholar 

  • MacNeil JD, Patterson J, Martz V (2000) Validation of analytical methods proving your method is “fit for purpose”. In: Fajgelj A, Ambrus A (eds) Principles and practices of method validation. RSC, Cambridge, p 305

    Google Scholar 

  • Quevauviller P, Morabito R (2000) Evaluation of extraction recoveries for organometallic determinations in environmental matrices. Trends Anal Chem 19:86–96

    Article  CAS  Google Scholar 

  • Rodrigues MV, Reyes FG, Magalhães PM, Rath S (2007) GC-MS determination of organochlorine pesticides in medicinal plants harvested in Brazil. J Brazilian Chem Soc 18:135–142

    Article  CAS  Google Scholar 

  • SANCO (2007) Method validation and quality control procedures for pesticide residue analysis in food and feed. Document no. SANCO/2007/3131

  • Sharma KK (2007) Pesticide residue analysis manual. ICAR, New Delhi

    Google Scholar 

  • Trullols E, Ruisánchez I, Rius FX, Òdena M, Feliu MT (2004) Qualitative method for determination of aflatoxin B1 in nuts. J AOAC Int 87:417–423

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Faculty of Science, University of Gondar, PO Box 196, Gondar, Ethiopia

    Amsalu Debebe

  2. Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore, TN, 641003, India

    S. Kuttalam

Authors
  1. Amsalu Debebe
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Kuttalam
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Amsalu Debebe.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Debebe, A., Kuttalam, S. Parameter Validation of Analytical Methods of Insecticide Residue Analyses in Foods of Animal Origin, Feed and Water. Bull Environ Contam Toxicol 86, 571–575 (2011). https://doi.org/10.1007/s00128-011-0284-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00128-011-0284-1

Keywords

Search

Navigation