A multi-location field trial was conducted in India during 2006–2008 to evaluate the dissipation pattern of thiophanate methyl (75% WP) in/on grapes at two application rates (500 and 1,000 g a.i. ha−1). The quantitative analysis of the fungicide residues as carbendazim was performed using a UV/VIS spectrophotometer at the maximum absorption band of 281 nm. The average recovery was found 87% and the relative standard deviations (RSD) were below 3.8%. Following the first order kinetics the fungicide dissipates in grapes with a half-life (t ½) value of 4.74–6.52 days irrespective of locations and doses.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Price includes VAT (USA)
Tax calculation will be finalised during checkout.
Abdel Megeed MI, Zidan ZH, Afifi FA, Al Naser ZA (2000) Residues of procymidone and thiophanate-methyl fungicides on tomatoes and cucumber under field and protected cultivations. Ann Agric Sci Cairo 4:1695–1709
Anonymous (2004) The prevention of food adulteration act: Ministry of Health & Family Welfare, Gazette of India, extraordinary, Part II, section 3
Banks D, Soliman MR (1997) Protective effects of antioxidants against benomyl-induced lipid peroxidation and glutathione depletion in rats. Toxicology 116:177–181
Buchenauer H, Erwin DC, Keen NT (1973a) Systemic fungicidal effects of thiophanate methyl on verticillium wilt of cotton and its transformation to methyl-2-benzimidazol carbamate in cotton plants. Phytopathology 63:1091
Buchenauer H, Edgin LN, Grassman F (1973b) Photochemical transformation of thiophanate methyl and thiophanate or alkyl benzimidazol-2yl-carbamate. Pestic Sci 4:343
Dutta P, Guha PK, Dhua RS, Chowdhury A, Bhattacharyya A (1995) Persistance of thiophanate methyl residue in/on mango. Pestology 19:20–22
FAOSTAT (2007) FAO Statistical Database. http://www.fao.org
Maranghi F, Macr C, Ricciardi C, Stazi AV, Rescia M, Mantovani A (2003) Histological and histomorphometric alterations in thyroid MT effects on Podarcis sicula adrenal glands 247 and adrenals of CD rat pups exposed in utero to methyl thiophanate. Reprod Toxicol 17:617–623
Muccio AD, Cammoni I, Ventriglia M, Barbini DA, Mauro M, Pelosi P, Generali T, Ausili A, Girolimetti S (1995) Simplified clean-up for the determination of benzimidazolic fungicides by high-performance liquid chromatography with UV detection. J Chromatogr A 697:145–152
Ono S (1973) Analytical method for residues of thiophanate-methyl and 2-methyl benzimidazole carbamate in crops by UV spectrometry. Nippon Soda Co Unpublished
Pezzuto JM (2008) Grapes and human health: a perspective. J Agric Food Chem 56:6777–6784
Soeda Y, Kosaka S, Noguchi T (1972) The fate of thiophanate methyl fungicide and its metabolite on plant leaves and glass plates. Agric Biol Chem 36:931
Traina ME, Fazzi P, Macr C, Ricciardi C, Stazi AV, Urbani E, Mantovani A (1998) In vivo studies on possible adverse effects on reproduction of the fungicide methyl thiophanate. J Appl Toxicol 18:241–248
Urani C, Chiesara E, Galvani P, Marabini L, Santagostino A, Camatini M (1995) Benomyl affects the microtubule cytoskeleton and the glutathione level of mammalian primary cultured hepatocytes. Toxicol Lett 76:135–144
Authors are thankful to M/S Biostadt India Ltd, Mumbai, India, for sponsoring this research program.
About this article
Cite this article
Mandal, S., Das, S. & Bhattacharyya, A. Dissipation Study of Thiophanate Methyl Residue in/on Grapes (Vitis vinifera L.) in India. Bull Environ Contam Toxicol 84, 592–595 (2010). https://doi.org/10.1007/s00128-010-9985-0
- Thiophanate methyl residue
- UV/VIS Spectrophotometer