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Toxicity Evaluation of a Conservation Effects Assessment Program Watershed, Beasley Lake, in the Mississippi Delta, USA


Beasley Lake was assessed monthly in 2005 for biological impairment from 17 historic and current-use pesticides in water and leaf litter using Hyalella azteca (Saussure). Sixteen pesticides were detected in both water and leaf litter with peak detections in spring and summer. Detections ranged from 1–125 ng L−1 in water and 1–539 ng g−1 OC in leaf litter. Ten-day H. azteca survival and growth (mg dw) bioassay results indicated no adverse effects on survival or growth in H. azteca exposed to water or leaf litter. Rather, enhanced growth occurred in both lake water and leaf litter exposures for 8 and 6 months, respectively.

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  1. American Public Health Association (APHA) (2005) Standard methods for the examination of water and wastewater, 21st edn. Washington, DC

    Google Scholar 

  2. Bennett ER, Moore MT, Cooper CM, Smith S (2000) Method for the simultaneous extraction and analysis of two current use pesticides, atrazine and lambda-cyhalothrin, in sediment and aquatic plants. Bull Environ Contam Toxicol 64:825–833

    Article  CAS  Google Scholar 

  3. Cooper C, Smith S, Moore M (2003) Surface water, ground water and sediment quality in three oxbow lake watersheds in the Mississippi delta agricultural region: pesticides. Int J Ecol Environ Sci 29:171–184

    Google Scholar 

  4. De March BGE (1981) Hyalella azteca (Saussure). In: Lawrence SG (ed) Manual for the culture of freshwater invertebrates. Canadian Spec Publ Fish Aquat Sci 54:61–77

  5. Deaver E, Rodgers JH (1996) Measuring bioavailable copper using anodic stripping voltammetry. Environ Toxicol Chem 15:1925–1930

    Article  CAS  Google Scholar 

  6. Locke MA, Knight SS, Smith S, Cullum RF, Zablotowicz RM, Yuan Y, Bingner RL (2008) Environmental quality research in the Beasley Lake watershed, 1995–2007: succession from conventional to conservation practices. J Soil Water Conserv 63:430–442. doi:10.2489/jswc.63.6.430

    Article  CAS  Google Scholar 

  7. Maul JD, Trimble AJ, Lydy MJ (2008) Partitioning and matrix-specific toxicity of bifenthrin among sediments and leaf-sourced organic matter. Environ Toxicol Chem 27:945–952

    Article  CAS  Google Scholar 

  8. Moore MT, Lizotte RE, Smith S (2007a) Reponses of Hyalella azteca to a pyrethroid mixture in a constructed wetland. Bull Environ Contam Toxicol 78:245–248. doi:10.1007/s00128-007-9135-5

    Article  CAS  Google Scholar 

  9. Moore MT, Lizotte RE, Knight SS, Smith S, Cooper CM (2007b) Assessment of pesticide contamination in three Mississippi delta oxbow lakes using Hyalella azteca. Chemosphere 67:2184–2191. doi:10.1016/j.chemosphere.2006.12.026

    Article  CAS  Google Scholar 

  10. Nett MT, Locke MA, Pennington DA (2004) Water quality assessments in the mississippi delta: regional solutions, national scope. ASC symposium series 877, Washington, DC

  11. Statistical Package for the Social Sciences (SPSS), Inc. (1997) SigmaStat for Windows version 2.03

  12. Steel RGD, Torrie JH, Dickey DA (1997) Principles and Procedures of Statistics: A Biometrical Approach. McGraw-Hill, Inc., New York p 665

    Google Scholar 

  13. Sundberg SE, Hassan SM, Rodgers JH (2006) Enrichment of elements in detritus from a constructed wetland and consequent toxicity to Hyalella azteca. Ecotox Environ Saf 64:264–272. doi:10.1016/j.ecoenv.2006.02.010

    Article  CAS  Google Scholar 

  14. US Environmental Protection Agency (USEPA) (2000) Methods for measuring the toxicity and bioaccumulation of sediment-associated contaminants with freshwater invertebrates. USEPA 600/R-99/064

  15. Zablotowicz RM, Locke MA, Krutz LJ, Lerch RN, Lizotte RE, Knight SS, Gordon RE, Steinriede RW (2006) Influence of watershed system management on herbicide concentrations in Mississippi Delta oxbow lakes. Sci Total Environ 370:552–560. doi:10.1016/j.scitotenv.2006.08.023

    Article  CAS  Google Scholar 

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Appreciation is extended to S. Smith, C. Bryant, V. Campbell, D. McChesney, L. Brooks, A. Delmastro, and L. Arbuckle for analytical assistance as well as C. Bryant and M. Haasch for reviewing an earlier version of the manuscript. Mention of equipment, software or a pesticide does not constitute an endorsement for use by the US Department of Agriculture nor does it imply pesticide registration under FIFRA as amended. All programs and services of the USDA are offered on a nondiscriminatory basis without regard to race, color, national origin, religion, sex, marital status, or handicap.

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Correspondence to Richard E. Lizotte Jr..

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Lizotte, R.E., Knight, S.S. & Cooper, C.M. Toxicity Evaluation of a Conservation Effects Assessment Program Watershed, Beasley Lake, in the Mississippi Delta, USA. Bull Environ Contam Toxicol 84, 422–426 (2010).

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  • CEAP
  • Pesticides
  • Lake water
  • Leaf litter
  • Hyalella azteca