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Evaluation of Pyrogallol-induced Cytotoxicity in Catalase-mutant Escherichia coli and Mutagenicity in Salmonella typhimurium


We evaluated pyrogallol cytotoxicity using Escherichia coli strains that express mammalian catalase gene derived from catalase mutant mice (Csb) and wild-type (Csa), and pyrogallol mutagenicity by Ames test. Pyrogallol was more toxic to Csb rather than to Csa (p < 0.05), while catalase, superoxide dismutase and ascorbic acid decrease the toxic effect. Pyrogallol also showed mutagenic effect (mutagenic index = 3.8 for 10 μmol pyrogallol/plate) while ascorbic acid (19.4% reduction, p < 0.001) and naringin (35.1% reduction, p < 0.001) played a protective role against it. Pyrogallol cytotoxicity and mutagenicity seem to be attributable, at least in part, to reactive oxygen species formation. This study also suggests that newly established catalase mutant E. coli is probably useful in hazard identification of oxidative chemicals.

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We would like to express our gratitude to Dr. P. C. Loewen for kindly supplying bacterial strain UM255. This work was supported by Grant-in-Aid for Science Research from the Ministry of Education, Science and Culture of Japan.

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Correspondence to Da-Hong Wang.

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Takemura, Y., Wang, DH., Sauriasari, R. et al. Evaluation of Pyrogallol-induced Cytotoxicity in Catalase-mutant Escherichia coli and Mutagenicity in Salmonella typhimurium . Bull Environ Contam Toxicol 84, 347–350 (2010).

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  • Catalase-mutant Escherichia coli
  • Pyrogallol
  • Cytotoxicity
  • Mutagenicity