Skip to main content

Evaluation of Pyrogallol-induced Cytotoxicity in Catalase-mutant Escherichia coli and Mutagenicity in Salmonella typhimurium

Abstract

We evaluated pyrogallol cytotoxicity using Escherichia coli strains that express mammalian catalase gene derived from catalase mutant mice (Csb) and wild-type (Csa), and pyrogallol mutagenicity by Ames test. Pyrogallol was more toxic to Csb rather than to Csa (p < 0.05), while catalase, superoxide dismutase and ascorbic acid decrease the toxic effect. Pyrogallol also showed mutagenic effect (mutagenic index = 3.8 for 10 μmol pyrogallol/plate) while ascorbic acid (19.4% reduction, p < 0.001) and naringin (35.1% reduction, p < 0.001) played a protective role against it. Pyrogallol cytotoxicity and mutagenicity seem to be attributable, at least in part, to reactive oxygen species formation. This study also suggests that newly established catalase mutant E. coli is probably useful in hazard identification of oxidative chemicals.

This is a preview of subscription content, access via your institution.

Fig. 1

References

  1. Brown JP (1980) A review of the genetic effects of naturally occurring flavonoids, anthraquinones and related compounds. Mutat Res 75:243–277

    CAS  Google Scholar 

  2. Do Céu Silva M, Gaspar J, Silva ID, Leão D, Rueff J (2003) Induction of chromosomal aberrations by phenolic compounds: possible role of reactive oxygen species. Mutat Res 540:29–42

    Google Scholar 

  3. Feinstein RN, Howard JB, Braun JT, Seaholm JE (1966) Acatalasemic and hypocatalasemic mouse mutants. Genetics 53:923–933

    CAS  Google Scholar 

  4. Glatt H, Padykula R, Berchtold GA, Ludewig G, Platt KL, Klein J, Oesch F (1989) Multiple activation pathways of benzene leading to products with varying genotoxic characteristics. Environ Health Perspect 82:81–89

    Article  CAS  Google Scholar 

  5. Grey CE, Adlercreutz P (2003) Ability of antioxidants to prevent oxidative mutations in Salmonella typhimurium TA102. Mutat Res 527:27–36

    CAS  Google Scholar 

  6. Horita M, Wang DH, Tsutsui K, Sano K, Masuoka N, Kira S (2005) Involvement of oxidative stress in hydroquinone- induced cytotoxicity in catalase-deficient Escherichia coli mutants. Free Radic Res 39:1035–1041

    Article  CAS  Google Scholar 

  7. Horváthová K, Chalupa I, Šebová L, Tóthová D, Vachálková A (2005) Protective effect of quercetin and luteolin in human melanoma HMB-2 cells. Mutat Res 565:105–112

    Google Scholar 

  8. Kanno S, Shouji A, Asou K, Ishikawa M (2003) Effects of naringin on hydrogen peroxide-induced cytotoxicity and apoptosis in P388 cells. J Pharmacol Sci 92:166–170

    Article  CAS  Google Scholar 

  9. Kessler M, Ubeaud G, Jung L (2003) Anti- and pro-oxidant activity of rutin and quercetin derivatives. J Pharm Pharmacol 55:131–142

    Article  CAS  Google Scholar 

  10. Lin JK, Lee SF (1992) Enhancement of the mutagenicity of polyphenols by chlorination and nitrosation in Salmonella typhimurium. Mutat Res 269:217–224

    CAS  Google Scholar 

  11. Marklund S, Marklund G (1974) Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European J Biochem 47:469–474

    Article  CAS  Google Scholar 

  12. Mortelmans K, Zeiger E (2000) The ames salmonella/microsome mutagenicity assay. Mutat Res 455:29–60

    CAS  Google Scholar 

  13. Nohynek GJ, Fautz R, Benech-Kieffer F, Toutain H (2004) Toxicity and human health risk of hair dyes. Food Chem Toxicol 42:517–543

    Article  CAS  Google Scholar 

  14. Smith J, Schrift A (1979) Phylogenic distribution of glutathione peroxidase. Comp Biochem Physiol 63:39–44

    CAS  Google Scholar 

  15. Wang DH, Tsutsui K, Sano K, Masuoka N, Kira S (2001) cDNA cloning and expression of mutant catalase from the hypocatalasemic mouse: comparison with the acatalasemic mutant. Biochim Biophys Acta 1522:217–220

    CAS  Google Scholar 

  16. Wang L, Tu YC, Lian TW, Hung JT, Yen JH, Wu MJ (2006) Distinctive antioxidant and antiinflammatory effects of flavonols. J Agric Food Chem 54:9798–9804

    Article  CAS  Google Scholar 

  17. Yahagi T, Nagao M, Seino Y, Matsushima T, Sugimura T (1977) Mutagenicities of N-nitrosamines on Salmonella. Mutat Res 48:121–129

    CAS  Google Scholar 

Download references

Acknowledgments

We would like to express our gratitude to Dr. P. C. Loewen for kindly supplying bacterial strain UM255. This work was supported by Grant-in-Aid for Science Research from the Ministry of Education, Science and Culture of Japan.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Da-Hong Wang.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Takemura, Y., Wang, DH., Sauriasari, R. et al. Evaluation of Pyrogallol-induced Cytotoxicity in Catalase-mutant Escherichia coli and Mutagenicity in Salmonella typhimurium . Bull Environ Contam Toxicol 84, 347–350 (2010). https://doi.org/10.1007/s00128-010-9936-9

Download citation

Keywords

  • Catalase-mutant Escherichia coli
  • Pyrogallol
  • Cytotoxicity
  • Mutagenicity