Stability of Mercury Concentrations in Frozen Avian Blood Samples


It is unclear whether mercury concentration in wildlife tissues changes appreciably after lengthy frozen storage. To test whether such freezer-archived samples are stable, small (~10–50 μL) avian blood samples stored in capped glass capillary tubes were analyzed for total mercury concentration, and then reanalyzed after being frozen for up to 3 years. Mercury concentrations increased 6% on average over the 3 year period, but time spent frozen explained only 11% of the variation between measurements. This small amount of change suggests that archived blood samples remain useful for at least several years.

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  1. Brasso RL, Cristol DA (2008) Effects of mercury exposure on the reproductive success of tree swallows (Tachycineta bicolor). Ecotoxicology 17:133–141

    CAS  Article  Google Scholar 

  2. Condon AM, Cristol DA (2009) Feather growth influences blood mercury level of young songbirds. Environ Toxicol Chem 28:395–401

    CAS  Article  Google Scholar 

  3. Cristol DA, Brasso RL, Condon AM, Fovargue RE, Friedman SL, Hallinger KK, Monroe AP, White AE (2008) The movement of aquatic mercury through terrestrial food webs. Science 320:335

    CAS  Article  Google Scholar 

  4. De Boer J, Smedes F (1997) Effects of storage conditions of biological materials on the contents of organochloride compounds and mercury. Mar Pollut Bull 35:93–108

    Article  Google Scholar 

  5. Devai I, Delaune RD, Patrick WH Jr, Gambrell RP (2001) Changes in methylmercury concentration during storage: effect of temperature. Org Geochem 32:755–758

    CAS  Article  Google Scholar 

  6. Hallinger KK, Cornell KL, Brasso RL, Cristol DA (2010) Mercury exposure and survival in free-living tree swallows (Tachycineta bicolor). Ecotoxicology (currently available online) (In Press)

  7. Horvat M, Byrne AR (1992) Preliminary study of the effects of some physical parameters on the stability of methylmercury in biological samples. Analyst 117:665–668

    CAS  Article  Google Scholar 

  8. LaFleur PD (1973) Retention of mercury when freeze-drying biological materials. Anal Chem 45:1534–1536

    CAS  Article  Google Scholar 

  9. Liang L, Evens C, Lazoff S, Woods JS, Cernichiari E, Horvat M, Martin MD, DeRouen T (2000) Determination of methyl mercury in whole blood by ethylation-GC-CVAFS after alkaline digestion-solvent extraction. J Anal Toxicol 24:328–332

    CAS  Google Scholar 

  10. Parker JL, Bloom NS (2005) Preservation and storage techniques for low-level aqueous mercury speciation. Sci Total Environ 337:253–263

    CAS  Article  Google Scholar 

  11. Peterson SA, Peck DV, Van Sickle J, Hughes RM (2007) Mercury concentration in frozen whole-fish homogenates is insensitive to holding time. Arch Environ Contam Toxicol 53:411–417

    CAS  Article  Google Scholar 

  12. Spěváčková V, Korunová V, Čejchanová M, Vobecký M (2004) Sampling procedure and a radio-indicator study of mercury determination in whole blood by using an AMA 254 atomic absorption spectrometer. Anal Bioanal Chem 380:346–350

    Article  Google Scholar 

  13. United States Environmental Protection Agency (1995) QA/QC guidance for sampling and analysis of sediments, water, and tissues for dredged material evaluations—chemical evaluations. EPA 823/B/95/001. US Environmental Protection Agency, Washington

    Google Scholar 

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We thank the South River Science Team. Funding was provided by E. I. DuPont de Nemours and Company, The Office of Vice Provost for Research at the College of William and Mary, National Science Foundation Grant UBM 0436318, and the William and Mary Undergraduate Science Education and Research Program sponsored by the Howard Hughes Medical Institute.

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Correspondence to Claire W. Varian-Ramos.

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Varian-Ramos, C.W., Condon, A.M., Hallinger, K.K. et al. Stability of Mercury Concentrations in Frozen Avian Blood Samples. Bull Environ Contam Toxicol 86, 159–162 (2011).

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  • Avian blood
  • Temporal stability
  • Mercury
  • Tissue storage