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Determination of the Effects of Genistein on the Longevity of Drosophila melanogaster Meigen (Diptera; Drosophilidae)

Abstract

In this study, the effects of genistein on the longevity of Drosophila melanogaster were investigated. The effects of different concentrations of genistein (1, 3, 5 and 10 μM/100 mL medium) were separately administered one by one to female and male populations of D. melanogaster for application groups. In the control group, the maximum life span was determined to be 57 days for ♀♀, 46 for ♂♂. The maximum life span for the lowest (1.0 μL) and highest (10.0 μL) application groups among the adult populations of D. melanogaster subjected to genistein were observed to be 54, 50, 40 and 36 days for ♀♀ and 51, 48, 40 and 33 days for ♂♂. These values indicate a negative correlation (R = 0.513 for ♂♂ and R = 0.509 for ♀♀) between the maximum life span of the application groups and changing genistein concentrations.

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References

  1. Adlercreutz H, Mazur W (1997) Phyto-oestrogens and western diseases. Ann Med 29:95–120

    CAS  Google Scholar 

  2. Adlercreutz H, Honjo H, Higashi A, Fotsis T, Hämäläinen E, Hasegawa T, Okada H (1991) Urinary excretion of lignans and isoflavonoid phytoestrogens in Japanese men and women consuming a traditional Japanese diet. Am J Clin Nutr 54:1093–1100

    CAS  Google Scholar 

  3. Aksakal E, Ceyhun SB, Erdoğan O, Ekinci D (2010) Acute and long-term genotoxicity of deltamethrin to insulin-like growth factors and growth hormone in rainbow trout. Comp Biochem Physiol C 152:451–455

    Google Scholar 

  4. Albertazzi P, Purdie D (2002) The nature and utility of the phytoestrogens: a review of the evidence. Maturitas 42:173–185

    CAS  Article  Google Scholar 

  5. An J, Tzagarakis-Foster C, Scharschmidt TC, Lomri N, Leitman DC (2001) Estrogen receptor beta-selective transcriptional activity and recruitment of coregulators by phytoestrogens. J Biol Chem 276:17808–17814

    CAS  Article  Google Scholar 

  6. Bedigian D (2007) Phytoestrogens in functional foods. Econ Bot 61:107

    Google Scholar 

  7. Ceyhun SB, Senturk M, Ekinci D, Erdogan O, Ciltas A, Kocaman M (2010) Deltamethrin attenuates antioxidant defense system and induces the expression of heat shock protein 70 in rainbow trout. Comp Biochem Physiol C 152:215–223

    Google Scholar 

  8. Colborn T, Dumanoski D, Myers JP (1996) Our stolen future. Penguin Books, New York

    Google Scholar 

  9. Davis SR, Dalais FS, Simpson ER, Murkies AL (1999) Phytoestrogens in health and disease. Recent Prog Horm Res 54:185–211

    CAS  Google Scholar 

  10. Duncan DB (1955) Multiple range and multiple F test. Biometrics 11:1–42

    Article  Google Scholar 

  11. Ekinci D, Beydemir S (2010) Risk assessment of pesticides and fungicides for acid-base regulation and salt transport in rainbow trout tissues. Pestic Biochem Phys 97:66–70

    CAS  Article  Google Scholar 

  12. Jefferson WN, Padilla-Banks E, Newbold RR (2007) Disruption of the developing female reproductive system by phytoestrogens: genistein as an example. Mol Nutr Food Res 51:832–844

    CAS  Article  Google Scholar 

  13. Kulling SE, Honig DM, Metzler M (2001) Oxidative metabolism of the soy isoflavones daidzein and genistein in humans in vitro and in vivo. J Agric Food Chem 49:3024–3033

    CAS  Article  Google Scholar 

  14. Lazzari G, Tessaro I, Crotti G, Gali C, Hoffmann S, Bremer S, Pellizzer C (2008) Development of an in vitro test battery for assessing chemical effects on bovine germ cells under the Reprotect umbrella. Toxicol Appl Pharmacol 233:360–370

    CAS  Article  Google Scholar 

  15. Le Bourg E (2001) Oxidative stress, aging and longevity in Drosophila melanogaster. FEBS Lett 498:183–186

    CAS  Article  Google Scholar 

  16. Liu ZH, Kanjo Y, Mizutani S (2010) A review of phytoestrogens: their occurrence and fate in the environment. Water Res 44:567–577

    CAS  Article  Google Scholar 

  17. Page MM, Robb EL, Salway KD, Stuart JA (2010) Mitochondrial redox metabolism: aging, longevity and dietary effects. Mech Ageing Dev 131:242–252

    CAS  Article  Google Scholar 

  18. Setchell KD, Zimmer-Nechemias L, Cai J, Heubi JE (1997) Exposure of infants to phyto-estrogens from soy-based infant formula. Lancet 350:23–27

    CAS  Article  Google Scholar 

  19. Stopper H, Schmitt E, Kobras K (2005) Genotoxicity of phytoestrogens. Mutat Res 574:139–155

    CAS  Google Scholar 

  20. Turner JV, Agatonovic-Kustrin S, Glass BD (2007) Molecular aspects of phytoestrogen selective binding at estrogen receptors. J Pharm Sci 96:1879–1885

    CAS  Article  Google Scholar 

  21. Wanibuchi H, Kang JS, Salim EI, Morimura K, Fukushima S (2003) Toxicity versus beneficial effects of phytoestrogenes. Pure Appl Chem 75:2047–2053

    CAS  Article  Google Scholar 

  22. Wu Z, Smith JV, Paramasivam V, Butko P, Khan I, Cypser JR, Luo Y (2002) Ginkgo biloba extract EGb 761 increases stress resistance and extends lifespan of Caenorhabditis elegans. Cell Mol Biol 48:725–731

    CAS  Google Scholar 

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Correspondence to Handan Uysal.

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Altun, D., Uysal, H., Aşkın, H. et al. Determination of the Effects of Genistein on the Longevity of Drosophila melanogaster Meigen (Diptera; Drosophilidae). Bull Environ Contam Toxicol 86, 120–123 (2011). https://doi.org/10.1007/s00128-010-0159-x

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Keywords

  • Aging
  • Drosophila melanogaster
  • Genistein
  • Longevity