Abstract
The antiviral drug Tamiflu has received particular attention because of its recommended use against the influenza A H5N1 and H1N1 viruses. Given its resistance to degradation and its hydrophilicity the active metabolite, Oseltamivir Carboxylate (OC), is expected to enter the aquatic ecosystem from sewage treatment plants. In the present paper the bacterial community of surface water samples, treated with OC (1.5 mg L−1), was characterized by Fluorescence In Situ Hybridization (FISH) in microcosm experiments. The α-, β- and γ-Proteobacteria increased in OC-treated versus non-treated water samples during the incubation period, suggesting these bacterial groups had an active role in OC degradation.
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Accinelli C, Barra Caracciolo A, Grenni P (2007) Degradation of the antiviral drug oseltamivir carboxylate in surface water samples. Int J Environ Anal Chem 87:579–587
Accinelli C, Saccà ML, Fick J, Mencarelli M, Lindberg R, Olsen B (2010) Dissipation and removal of oseltamivir (Tamiflu) in different aquatic environments. Chemosphere 79:891–897
Alonso JL, Mascellaro S, Moreno Y, Ferrus MA, Hernandez J (2002) Double-staining method for differentiation of morphological changes and membrane integrity of Campylobacter coli cells. Appl Environ Microbiol 68:5151–5154
Barra Caracciolo A, Grenni P, Cupo C, Rossetti S (2005) In situ analysis of native microbial communities in complex samples with high particulate loads. FEMS Microbiol Lett 253:55–58
Barra Caracciolo A, Grenni P, Saccà ML, Amalfitano S, Ciccoli R, Martín M, Gibello A (2010) The role of a groundwater bacterial community in the degradation of the herbicide terbuthylazine. FEMS Microbiol Ecol 71(1):127–136
Bartels P, von Tümpling W Jr (2008) The environmental fate of the antiviral drug oseltamivir carboxylate in different waters. Sci Total Environ 405:215–225
Fick J, Lindberg RH, Tysklind M, Haemig PD, Waldenström J, Wallensten A, Olsen B (2007) Antiviral oseltamivir is not removed or degraded in normal sewage water treatment: implications for development of resistance by influenza A virus. PLoS ONE 2:e986
Grenni P, Gibello A, Barra Caracciolo A, Fajardo C, Nande M, Vargas R, Saccà ML, Martínez-Iñigo MJ, Ciccoli R, Martín M (2009) A new fluorescent oligonucleotide probe for in situ detection of s-triazine-degrading Rhodococcus wratislaviensis in contaminated groundwater and soil samples. Water Res 43:2999–3008
Loy A, Maixner F, Wagner M, Horn M (2007) probeBase–an online resource for rRNA-targeted oligonucleotide probes: new features 2007. Nucleic Acids Res 35(Database issue):D800–D804
Saccà ML, Accinelli C, Fick J, Lindberg R, Olsen B (2009) Environmental fate of the antiviral drug Tamiflu in two aquatic ecosystems. Chemosphere 75:28–33
Singer AC, Nunn MA, Gould EA, Johnson AC (2007) Potential risks associated with the widespread use of Tamiflu. Environ Health Perspect 115:102–106
Singer AC, Howard BM, Johnson AC, Knowles CJ, Jackman S, Accinelli C, Barra Caracciolo A et al (2008) Meeting report: risk assessment of Tamiflu use under pandemic conditions. Environ Health Perspect 116(11):1563–1567
Straub JO (2009) An environmental risk assessment for oseltamivir (Tamiflu®) for sewage works and surface waters under seasonal-influenza-and pandemic-use conditions. Ecotoxicol Environ Saf 72:1625–1634
Ward P, Small I, Smith J, Suter P, Dutkowski R (2005) Oseltamivir (Tamiflu) and its potential for use in the event of an influenza pandemic. J Antimicrob Chemother 55:i5–i21
Zwart G, Crump BC, Kamst-van Agterveld MP, Hagen F, Han SK (2002) Typical freshwater bacteria: an analysis of available 16S rRNA gene sequences from plankton of freshwater lakes and rivers. Aquat Microb Ecol 28:141–155
Acknowledgments
Funding for this study was provided by the “Italian Environmental Ministry” (Project: The Exposure Scenario and Risk Potential of Biocides). The authors thank particularly Francesca Falconi for her useful help in microbiological and DOC analysis.
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Barra Caracciolo, A., Grenni, P. & Saccà, M.L. Effect of the Antiviral Drug Oseltamivir (Tamiflu) on the Bacterial Community Structure of a Surface Water Ecosystem Analyzed Using Fluorescence In Situ Hybridization. Bull Environ Contam Toxicol 85, 443–446 (2010). https://doi.org/10.1007/s00128-010-0114-x
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DOI: https://doi.org/10.1007/s00128-010-0114-x