Effects of Glyphosate and 2,4-D on Earthworms (Eisenia foetida) in Laboratory Tests

  • F. V. CorreiaEmail author
  • J. C. Moreira


Laboratory tests were conducted to compare the effects of various concentrations of glyphosate and 2,4-D on earthworms (Eisenia foetida) cultured in Argissol during 56 days of incubation. The effects on earthworm growth, survival, and reproduction rates were verified for different exposure times. Earthworms kept in glyphosate-treated soil were classified as alive in all evaluations, but showed gradual and significant reduction in mean weight (50%) at all test concentrations. For 2,4-D, 100% mortality was observed in soil treated with 500 and 1,000 mg/kg. At 14 days, 30%–40% mortality levels were observed in all other concentrations. No cocoons or juveniles were found in soil treated with either herbicide. Glyphosate and 2,4-D demonstrated severe effects on the development and reproduction of Eisenia foetida in laboratory tests in the range of test concentrations.


Terrestrial ecotoxicology Earthworm Eisenia foetida Contaminated soils Herbicides 



The study was funded by the Brazilian National Research Council (CNPq, Grant 555193) and the Rio de Janeiro State Research Foundation (FAPERJ, Call for Projects 10/2009).


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© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Ecotoxicology LaboratoryCenter for Studies on Workers’ Health and Human EcologyManguinhosBrazil

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