Skip to main content

Degradation of Di-n-butyl Phthalate by Newly Isolated Ochrobactrum sp.

Abstract

A gram negative isolate designated JDC-41 was obtained from river sludge using mixtures of phthalate esters as the sole source and energy. The isolate was identified as Ochrobactrum sp. based on its 16S rRNA gene sequence. Over 87% of supplied di-n-butyl phthalate (DBP) was degraded by JDC-41 in a pH neutral mineral salts medium at 30°C within 48 h. Increased DBP (50–500 mg/L) in the culture correspondingly increased degradation half-life from 3.83 to 18.12 h. DBP induced cells more rapidly degraded DBP.

This is a preview of subscription content, access via your institution.

Fig. 1

References

  1. Chang BV, Yang CM, Cheng CH, Yuan SY (2004) Biodegradation of phthalate esters by two bacteria strains. Chemosphere 55:533–538

    CAS  Article  Google Scholar 

  2. Chao WL, Lin CM, Shiung II, Kuo YL (2006) Degradation of di-butyl-phthalate by soil bacteria. Chemosphere 63:1377–1383

    CAS  Article  Google Scholar 

  3. Hashizume K, Nanya J, Toda C, Yasui T, Nagano H, Kojima N (2002) Phthalate esters detected in various water samples and biodegradation of the phthalates by microbes isolated from river water. Biol Pharm Bull 25:209–214

    CAS  Article  Google Scholar 

  4. Liang DW, Zhang T, Fang HHP, He JZ (2008) Phthalates biodegradation in the environment. Appl Microbiol Biotechnol 80:183–198

    CAS  Article  Google Scholar 

  5. Lottrup G, Andersson A, Leffers H, Mortensen G, Toppari J, Skakkebaek N, Main K (2006) Possible impact of phthalates on infant reproductive health. Int J Androl 29:172–180

    CAS  Article  Google Scholar 

  6. Ogawa G, Ishida M, Urano N (2009) Isolation and identification of dibutyl phthalate-degrading bacteria from hydrospheres in Tokyo. J Gen Appl Microbiol 55:261–265

    CAS  Article  Google Scholar 

  7. Patil NK, Kundapur R, Shouche YS, Karegoudar TB (2006) Degradation of plasticizer Di-n-butylphthalate by Delftia sp. TBKNP-05. Curr Microbiol 52:369–374

    CAS  Article  Google Scholar 

  8. Psillakis E, Mantzavinos D, Kalogerakis N (2004) Monitoring the sonochemical degradation of phthalate esters in water using solid-phase microextraction. Chemosphere 54:849–857

    CAS  Article  Google Scholar 

  9. Sun XR, Shan ZJ (2007) Removal of di-n-butyl phthalate using immobilized microbial cells. Chin J Chem Eng 15:167–171

    CAS  Article  Google Scholar 

  10. Sung HH, Kao WY, Su YJ (2003) Effects and toxicity of phthalate esters to hemocytes of giant freshwater prawn, Macrobrachium rosenbergii. Aquat Toxicol 64:25–37

    CAS  Article  Google Scholar 

  11. Vamsee-Krishna C, Mohan Y, Phale P (2006) Biodegradation of phthalate isomers by Pseudomonas aeruginosa PP4, Pseudomonas sp. PPD and Acinetobacter lwoffii ISP4. Appl Microbiol Biotechnol 72:1263–1269

    CAS  Article  Google Scholar 

  12. Wu XL, Liang RX, Dai QY, Jin DC, Wang YY, Chao WL (2009) Complete degradation of di-n-octyl phthalate by biochemical cooperation between Gordonia sp. strain JDC-2 and Arthrobacter sp. strain JDC-32 isolated from activated sludge. J Hazard Mater. doi:10.1016/j.jhazmat.2009.11.022

  13. Xu XR, Li HB, Gu JD (2005) Biodegradation of an endocrine-disrupting chemical di-n-butyl phthalate ester by Pseudomonas fluorescens B-1. Int Biodeterior Biodegrad 55:9–15

    CAS  Article  Google Scholar 

  14. Zhang XH, Zhang GS, Zhang ZH, Xu JH, Li SP (2006) Isolation and characterization of a Dichlorvos-Degrading strain DDV-1 of Ochrobactrum sp. Pedosphere 16:64–71

    Article  Google Scholar 

  15. Zhou QH, Wu ZB, Cheng SP, He F, Fu GP (2005) Enzymatic activities in constructed wetlands and di-n-butyl phthalate (DBP) biodegradation. Soil Biol Biochem 37:1454–1459

    CAS  Article  Google Scholar 

Download references

Acknowledgments

This work was supported by a grant from the National Natural Science Foundation of China (no. 30770388).

Author information

Affiliations

Authors

Corresponding authors

Correspondence to Xue-ling Wu or Wei-liang Chao.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Wu, Xl., Wang, Yy., Liang, Rx. et al. Degradation of Di-n-butyl Phthalate by Newly Isolated Ochrobactrum sp.. Bull Environ Contam Toxicol 85, 235–237 (2010). https://doi.org/10.1007/s00128-010-0080-3

Download citation

Keywords

  • Biodegradation
  • Di-n-butyl phthalate
  • Kinetics
  • Ochrobactrum sp.