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Degradation of Di-n-butyl Phthalate by Newly Isolated Ochrobactrum sp.


A gram negative isolate designated JDC-41 was obtained from river sludge using mixtures of phthalate esters as the sole source and energy. The isolate was identified as Ochrobactrum sp. based on its 16S rRNA gene sequence. Over 87% of supplied di-n-butyl phthalate (DBP) was degraded by JDC-41 in a pH neutral mineral salts medium at 30°C within 48 h. Increased DBP (50–500 mg/L) in the culture correspondingly increased degradation half-life from 3.83 to 18.12 h. DBP induced cells more rapidly degraded DBP.

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This work was supported by a grant from the National Natural Science Foundation of China (no. 30770388).

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Correspondence to Xue-ling Wu or Wei-liang Chao.

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Wu, Xl., Wang, Yy., Liang, Rx. et al. Degradation of Di-n-butyl Phthalate by Newly Isolated Ochrobactrum sp.. Bull Environ Contam Toxicol 85, 235–237 (2010).

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  • Biodegradation
  • Di-n-butyl phthalate
  • Kinetics
  • Ochrobactrum sp.