Differentially expressed genes (DEGs) in the head of medaka (Oryzias latipes) were investigated to assess the biological effects of benzo[a]pyrene (BaP) on genomic alterations. Eight and ten DEGs were identified in response to short (48 h) and long-term (15 days) exposure to BaP (25 μg/L), respectively, using annealing control primer-based polymerase chain reaction. Among the DEGs, matrilin 1 and prothrombin were commonly detected, suggesting that BaP exposure can alter the expression of genes associated with the formation of extracellular matrix and blood coagulation in the head of medaka.
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Adamo RD, Pelosi S, Trotta P, Sansone G (1997) Bioaccumulation and biomagnification of polycyclic aromatic hydrocarbons in aquatic organisms. Mar Chem 56:45–49
Aravindakshan J, Paquet V, Gregory M, Dufresne J, Fournier M, Marcogliese DJ, Cyr DG (2004) Consequences of xenoestrogen exposure on male reproductive function in spottail shiners (Notropis hudsonius). Toxicol Sci 78:156–165
Butenas S, Mann KG (2002) Blood coagulation. Biochemistry Mosc 67:3–12
Degen SJ, Sun WY (1998) The biology of prothrombin. Crit Rev Eukaryot Gene Expr 8:203–224
Guillette LJ Jr, Crain DA, Ronney AA, Pickford DB (1995) Organization versus activation: The role of endocrine disrupting contaminants (EDCs) during embryonic development in wildlife. Environ Health Perspect 103:157–164
Hylland K (2006) Polycyclic aromatic hydrocarbon (PAH) ecotoxicology in marine ecosystems. J Toxicol Environ Health Part A 69:109–123
IARC (1987) IARC monographs on the evaluation of the carcinogenic risk of chemicals to humans. Overall evaluation of carcinogenicity: an updating of IAPC monographs. vol. 1–42. Suppl. 7. International Agency for Research on Cancer, Lyon
Inoue K, Takei Y (2003) Asian medaka fishes offer new models for studying mechanisms of seawater adaptation. Comp Biochem Physiol B 136:635–645
Kim YJ, Kwak CI, Gu YY, Hwang IT, Chun JY (2004) Annealing control primer system for identification of differentially expressed genes on agarose gels. BioTechniques 36:424–434
Muratoglu S, Bachrati C, Malpeli M, Szabó P, Neri M, Dozin B, Deák F, Cancedda R, Kiss I (1995) Expression of the cartilage matrix protein gene at different chondrocyte developmental stages. Eur J Cell Biol 68:411–419
Park GS, Yoon SJ, Lee SM, Kim AH, Park SY, Kang DY (2005) Japanese medaka, Oryzias latipes as a test animal for marine ecotoxicological evaluation. Korean J Environ Biol 23:293–303
Wagener R, Ehlen HW, Ko YP, Kobbe B, Mann HH, Sengle G, Paulsson M (2005) The matrilins-adaptor proteins in the extracellular matrix. FEBS Lett 579:3323–3329
Walsh PN (2007) Prothrombin is a cofactor for the binding of factor XI to the platelet surface and for platelet-mediated factor-XI activation by thrombin. Biochemistry 46:12886–12887
This study was funded by the National Fisheries Research and Development Institute, Pusan, Korea.
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Oh, J.H., Moon, HB. & Choe, E.S. Alterations in Differentially Expressed Genes in the Head of Oryzias latipes Following Benzo[a]pyrene Exposure. Bull Environ Contam Toxicol 84, 682–686 (2010). https://doi.org/10.1007/s00128-010-0012-2
- Polycyclic aromatic hydrocarbon
- Environmental pollutant