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Detection of Estrogenic Activity from Kraft Mill Effluents by the Yeast Estrogen Screen

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Estrogenic activity of kraft pulp mill effluents (P. radiata, E. globulus and mixed −50% E. globulus and 50% P. radiata) was evaluated by the yeast estrogen screen assay. The estrogenic activity values were relatively low, ranking between 1.475 and 0.383 ng/L of EE2 eq. (Estrogenic equivalent of 17 α-ethynylestradiol), where the highest value corresponds to the E. globulus effluent and the lowest value to the P. radiata effluent. Analysis by solid phase extraction (SPE) and gas chromatography—mass spectrometry (GC-MS) of chemical compounds present in all three effluents detected at least five major groups of organic compounds, corresponding to fatty acids, hydrocarbons, phenols, sterols and triterpenes. Comparison of analytical and biological data suggests that sterols could be the cause of the estrogenic activity in the evaluated effluent.

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This work was partially supported by Fondecyt Grant No. 1070509 and a CONICYT AT 24080124 grant for S. Chamorro’s Ph.D. dissertation. Authors thank Prof. J P Sumpter (GLAXO Research and Development Limited, Brunel University) for providing us the S. cerevisiae recombinant. Authors thank CONICYT-CSIC grant (Folio: 2007-138).

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Correspondence to G. Vidal.

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Chamorro, S., Hernández, V., Monsalvez, E. et al. Detection of Estrogenic Activity from Kraft Mill Effluents by the Yeast Estrogen Screen. Bull Environ Contam Toxicol 84, 165–169 (2010).

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