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Levels of DNA Adducts in the Blood and Follicular Fluid of Women Undergoing In Vitro Fertilization Treatment and Its Correlation with the Pregnancy Outcome

Abstract

This study is designed to investigate the impact of DNA damage on pregnancy and fertilization rate outcome in a sub-sample of women undergoing IVF treatment. Blood and follicular fluid samples (n = 60) were analyzed for DNA adducts. While no BPDE-DNA adducts were detected, other unknown lipophilic adducts were seen in blood and follicular fluid. Women who failed to achieve pregnancy had higher DNA adducts in follicular fluid than those who succeeded (p < 0.05). Follicular fluid cotinine levels were associated with DNA adduct levels in blood and follicular fluid (p < 0.05). Evaluation of DNA damage resulting from oxidative stress could have a role in predicting IVF success rate.

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Acknowledgments

The authors are thankful to the staff of the IVF clinic and participants for their cooperation during the study. They are; also, indebted to King Faisal Specialist Hospital and Research Centre for its support (RAC#2010 006).

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Correspondence to Iman Al-Saleh.

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Al-Saleh, I., El-Doush, I., Arif, J. et al. Levels of DNA Adducts in the Blood and Follicular Fluid of Women Undergoing In Vitro Fertilization Treatment and Its Correlation with the Pregnancy Outcome. Bull Environ Contam Toxicol 84, 23 (2010). https://doi.org/10.1007/s00128-009-9889-z

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Keywords

  • DNA adducts
  • 32P-Postlabeling technique
  • In vitro fertilization
  • Saudi Arabia
  • Pregnancy outcome
  • Fertilization rate