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Arsenic Resistant Bacteria Isolated from Arsenic Contaminated River in the Atacama Desert (Chile)

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Abstract

In this study, arsenic resistant bacteria were isolated from sediments of an arsenic contaminated river. Arsenic tolerance of bacteria isolated was carried out by serial dilution on agar plate. Redox abilities were investigated using KMnO4. arsC and aox genes were detected by PCR and RT-PCR, respectively. Bacterial populations were identified by RapID system. Forty nine bacterial strains were isolated, of these, 55 % corresponded to the reducing bacteria, 4% to oxidizing bacteria, 8% presented both activities and in 33% of the bacteria none activity was detected. arsC gene was detected in 11 strains and aox genes were not detected. The activity of arsenic transforming microorganisms in river sediment has significant implications for the behavior of the metalloid.

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References

  • Al-Abed SR, Jegadeesan G, Purandare J, Allen D (2007) Arsenic release from iron rich mineral processing waste: influence of pH and redox potential. Chemosphere 66:775–782

    Article  CAS  Google Scholar 

  • Anderson C, Cook G (2004) Isolation and characterization of arsenate-reducing bacteria from arsenic-contaminated sites in New Zealand. Curr Microbiol 48:341–347

    Article  CAS  Google Scholar 

  • Battaglia-Brunet F, Dictor MC, Garrido F, Crouzet C, Morin D, Dekeyser K, Clarens M, Baranger P (2002) An arsenic(III)-oxidizing bacterial population: selection, characterization and performance in reactors. J Appl Microbiol 93:656–667

    Article  CAS  Google Scholar 

  • Chang JS, Yoon IH, Kim KW (2007) Isolation and ars detoxification of arsenite-oxidizing bacteria from abandoned arsenic-contaminated mines. J Microbiol Biotech 17:812–821

    CAS  Google Scholar 

  • Ellis PJ, Conrads T, Hille R, Kuhn P (2001) Crystal structure of the 100 kDa arsenite oxidase from Alcaligenes faecalis in two crystal forms at 1.64 A and 2.03 A. Structure 9:125–132

    Article  CAS  Google Scholar 

  • Ford T, Jay J, Patel A, Kile M, Prommasith P, Galloway T, Sanger R, Smith K, Depledge M (2005) Use of ecotoxicological tools to evaluate the health of new Bedford harbor sediments: a microbial biomarker approach. Environ Health Perspect 113:186–191

    Article  CAS  Google Scholar 

  • Jackson CR, Dugas SL (2003) Phylogenetic analysis of bacterial and archael arsC gene sequences suggest an ancient, common origin for arsenate reductase. BMC Evol Biol 3:18–23

    Article  Google Scholar 

  • Jackson CR, Harrison KG, Dugas SL (2005) Enumeration and characterization of culturable arsenate resistant bacteria in a large estuary. Syst Appl Microbiol 28:727–734

    Article  CAS  Google Scholar 

  • Macur RE, Colin J, Botero I, Mc Dermott TR, Inskeep W (2004) Bacterial populations associated with the oxidation and reduction of arsenic in an unsaturated soil. Environ Sci Technol 38:101–111

    Article  CAS  Google Scholar 

  • Muller D, Lièvremont D, Simeonova D, Hubert JC, Lett MC (2003) Arsenite oxidase aox genes from a metal-resistant B-proteobacterium. J Bacteriol 185:135–141

    Article  CAS  Google Scholar 

  • Neff JM (1997) Ecotoxicology of arsenic in marine environment. Environ Toxicol Chem 16:917–927

    Article  CAS  Google Scholar 

  • Ordoñez E, Letek M, Valbuena N, Gil JA, Mateos LM (2005) Analysis of genes involved in arsenic resistance in Corynebacterium glutamicum ATCC 13032. Appl Environ Microbiol 71:6206–6215

    Article  CAS  Google Scholar 

  • Queirolo F, Stegen S, Mondaca J, Cortes R, Rojas R, Contreras C, Muñoz L, Schwuger MJ, Ostapczuk P (2000) Total arsenic, lead, cadmium, copper and zinc in some salt rivers in the northern Andes of Antofagasta, Chile. Sci Total Environ 255:75–84

    Article  CAS  Google Scholar 

  • Rokbani A, Bauda P, Billard P (2007) Diversity of arsenite transporter genes from arsenic-resistant soil bacteria. Res Microbiol 158:128–137

    Article  CAS  Google Scholar 

  • Salmassi TM, Venkateswaren K, Satomi M, Nealson K, Newman D, Hering J (2002) Oxidation of arsenite by Agrobacterium albertimagni, AOL15, sp. Nov, isolated from Hotcreek, California. Geomicrobiol J 19:53–66

    Article  CAS  Google Scholar 

  • Saltikov C, Olson B (2002) Homology of Escherichia coli R773 arsA, arsB, and arsC genes in arsenic-resistant bacteria isolated from raw sewage and arsenic-enriched creek waters. Appl Environ Microbiol 68:280–288

    Article  CAS  Google Scholar 

  • Silver S, Phung T (2005) Genes and enzymes involved in bacterial oxidation and reduction of inorganic arsenic. Appl Environ Microbiol 71:599–608

    Article  CAS  Google Scholar 

  • Smedley PL, Kinniburgh DG (2002) A review of the source, behaviour and distribution of arsenic in natural waters. Appl Geochem 17:517–568

    Article  CAS  Google Scholar 

  • Taerakul P, Sun P, Golightly DW, Walker HW, Weavers LK, Zand B, Butalia T, Thomas TJ, Gupta H, Fan LS (2007) Characterization and re-use potential of by-products generated from the Ohio state carbonation and ash reactivation (OSCAR). Process Fuel 86:541–553

    Article  CAS  Google Scholar 

  • Valenzuela C, Campos VL, Yañez J, Zaror C, Mondaca MA (2008) Isolation of arsenite-oxidizing bacteria from arsenic-enriched sediments from Camarones river, northern Chile. Bull Environ Contam Toxicol 82:593–596

    Article  CAS  Google Scholar 

  • Yañez J, Fierro V, Mansilla H, Figueroa L, Cornejo L, Barnes R (2005) Arsenic speciation in human hair: a new perspective for epidemiological assessment in chronic arsenicism. J Environ Monit 7:1335–1341

    Article  CAS  Google Scholar 

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Acknowledgments

This work has been supported by Dirección de Investigación, Universidad de Concepción (Proyect N° 204.036.027-1.0) and FONDECYT (Proyect N° 1050088).

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Authors and Affiliations

  1. Microbiology Department, University of Concepción, P.O. Box 160-C, Correo 3, Concepción, Chile

    G. Escalante, V. L. Campos, C. Valenzuela & M. A. Mondaca

  2. Chemical Inorganic and Analytical Department, University of Concepción, Concepción, Chile

    J. Yañez

  3. Chemical Engeneering Department, University of Concepción, Concepción, Chile

    C. Zaror

  4. Environmental Science Center EULA-CHILE, University of Concepción, Concepción, Chile

    V. L. Campos

Authors
  1. G. Escalante
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  2. V. L. Campos
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  3. C. Valenzuela
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  4. J. Yañez
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  5. C. Zaror
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  6. M. A. Mondaca
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Corresponding author

Correspondence to M. A. Mondaca.

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Escalante, G., Campos, V.L., Valenzuela, C. et al. Arsenic Resistant Bacteria Isolated from Arsenic Contaminated River in the Atacama Desert (Chile). Bull Environ Contam Toxicol 83, 657–661 (2009). https://doi.org/10.1007/s00128-009-9868-4

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  • DOI: https://doi.org/10.1007/s00128-009-9868-4

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